Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği

Pınarbaş Çetin, Müberra; tarakçı, cemre; BOZDEVECİ, ARİF; Veyisoğlu, Gökhan; Alpay Karaoğlu, Şengül

Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği

Arif BOZDEVECİ, (Recep Tayyip Erdoğan Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Şengül ALPAY KARAOĞLU, (Recep Tayyip Erdoğan Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Müberra PINARBAŞ, (Recep Tayyip Erdoğan Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Cemre TARAKÇI, (Recep Tayyip Erdoğan Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Gökhan VEYİSOĞLU (Recep Tayyip Erdoğan Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES

3 0

Yıl: 2019 Cilt: 4 Sayı: 3 Sayfa Aralığı: 302 - 311 Metin Dili: Türkçe İndeks Tarihi: 27-01-2021

Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği

Öz:

Çalışmada çay toprağından izole edilen Trichoderma harzianum ID11C suşunun bazı enzim aktivitesi, ağır metallere toleransı,tohum (domates, mısır ve fasulye) çimlenmesi üzerine etkinliği ve fasulyede Rhizoctonia solani B227 (AG-4)'nin biyotik stresine karşıbiyokontrol aktivitesinin araştırılması amaçlandı. T. harzianum ID11C suşunun bazı enzim aktiviteleri kromojenik yöntemle incelendi. Sonuçlarfarklı konsantrasyonlarda ağır metal (Cu, Pb, Zn ve Cd) ve tuz toleransı olduğunu gösterdi. Trichoderma harzianum ID11C ve Rhizoctoniasolani B227(AG4) izolatının fasulye gelişimine karşın patojenite testi ve T. harzianum ID11C suşunun tohum çimlenme başarısına olanetkinliği, su agar metodu ile incelendi. T. harzianum ID11C ve R. solani B227 varlığında fasulye gelişim parametreleri saksı deneyi ilearaştırıldı. Fizyolojik (ana kök ve gövde uzunluğu, saçaklanma sayısı, yaprak sayısı, yaş ve kuru ağırlıkları) parametreler ölçüldü ve istatistikselanalizleri yapıldı.Trichoderma harzianum ID11C suşu, yüksek konsantrasyonlarda ağır metallere (100-400 mM) ve tuza (250 mM) karşı toleranslıdır.Bitki gelişimini teşvik eden enzim aktivitelerinin var olduğu gözlendi. T. harzianum suşunun tohumların çimlenme başarısı üzerinde kontrolekıyasla olumsuz bir etkisinin olmadığı tespit edildi. Saksı deneyinde, kontrol ile R. solani B227 grupları birbirine kıyaslandığında, gövde ve anakök uzunluğu, saçaklanma sayısı ve kökte lezyon skala değerleri ile yapılan istatistik analizi sonucu aralarında anlamlı farkın (p< 0.05, Tukey)olduğu belirlendi. R. solani B227 ve T. harzianum ID11D’nin ayrı ayrı fasulye gelişimi üzerine olan etkileri, kontrol grubuna göre iyi olmadığıbelirlendi. Ancak ID11C ve B227+ID11C grupları B227 grubu ile kıyaslandığında, patojenik etkinliği azalttığı belirlendi.R. solani B227 (AG4) izolatı, fasulyede (Zulbiye) patojeniteye neden olduğu, T. harzianum ID11C suşunun ise istatistiksel olarakolumlu yönde anlamlı bir fark oluşturmamasına rağmen, tüm parametrelerde patojeniteyi azalttığı belirlendi. T. harzianum ID11C suşununfasulye tarımında Rhizoctonia solani’ye karşı biyokontrol ve bitki destekleyici ajan olarak kullanılabileceği sonucuna varıldı.

Anahtar Kelime:

The Biocontrol Activity of Trichoderma harzianum ID11C Against to the Biotic Stress of Rhizoctonia solani B227 in Bean Development

Öz:

The aim of the study was to determine the efficacy of Trichoderma harzianum ID11C strain isolated from tea soil on germination of seeds (tomato, maize, and bean) and to investigate the biocontrol efficacy against biotic stress of Rhizoctonia solani B227(AG4) in beans. Some enzyme activities of T. harzianum ID11C strain were investigated by the chromogenic method. It was determined that it had a tolerance to heavy metals (Cu, Pb, Zn, and Cd) and salt in different concentrations. The results showed different concentration of some heavy metal (Cu, Pb, Zn and Cd) resistance and salt tolerance. The pathogenicity test against the bean development of the Rhizoctonia solani isolate and the efficiency of the T. harzianum strain to seed germination success were determined by the water agar method. In the presence of T. harzianum and Rhizoctonia solani, bean development parameters were investigated by pot experiment. Physiological parameters (root and stem length, number of hair root, leaf surface area and number, wet and dry weights, etc.) were measured and then statistical analyses were performed. Trichoderma harzianum ID11C strain was determined to tolerant against to high concentrations of heavy metals (100-400 mM) and salt (250 mM). It was observed that there were enzyme activities that promote plant growth. It was determined that T. harzianum strain had no negative effect on the germination success of the seeds compared to the control. In the pot experiment, when the control group and R. solani groups were compared with each other, it was determined that there was a significant difference between the results of the statistical analysis performed with the stem and main root length, the number of lateral root and the root lesion scale (Tukey test, p<0.05). The effects of R. solani or T. harzianum on bean development were not as good as the control group. However, ID11C and B227+ID11C groups were found to reduce pathogenic activity when compared with group B227. It was determined that R. solani caused pathogenesis in the bean (Zulbiye), whereas T. harzianum strain decreased pathogenicity in all parameters, although it is not statistically significant. It was concluded that T. harzianum ID11C strain could be used as biocontrol and plant supporting agent against Rhizoctonia solani in bean farming.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BOZDEVECİ A, Alpay Karaoğlu Ş, Pınarbaş Çetin M, tarakçı c, Veyisoğlu G (2019). Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. , 302 - 311.
Chicago	BOZDEVECİ ARİF,Alpay Karaoğlu Şengül,Pınarbaş Çetin Müberra,tarakçı cemre,Veyisoğlu Gökhan Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. (2019): 302 - 311.
MLA	BOZDEVECİ ARİF,Alpay Karaoğlu Şengül,Pınarbaş Çetin Müberra,tarakçı cemre,Veyisoğlu Gökhan Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. , 2019, ss.302 - 311.
AMA	BOZDEVECİ A,Alpay Karaoğlu Ş,Pınarbaş Çetin M,tarakçı c,Veyisoğlu G Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. . 2019; 302 - 311.
Vancouver	BOZDEVECİ A,Alpay Karaoğlu Ş,Pınarbaş Çetin M,tarakçı c,Veyisoğlu G Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. . 2019; 302 - 311.
IEEE	BOZDEVECİ A,Alpay Karaoğlu Ş,Pınarbaş Çetin M,tarakçı c,Veyisoğlu G "Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği." , ss.302 - 311, 2019.
ISNAD	BOZDEVECİ, ARİF vd. "Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği". (2019), 302-311.

APA	BOZDEVECİ A, Alpay Karaoğlu Ş, Pınarbaş Çetin M, tarakçı c, Veyisoğlu G (2019). Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 4(3), 302 - 311.
Chicago	BOZDEVECİ ARİF,Alpay Karaoğlu Şengül,Pınarbaş Çetin Müberra,tarakçı cemre,Veyisoğlu Gökhan Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 4, no.3 (2019): 302 - 311.
MLA	BOZDEVECİ ARİF,Alpay Karaoğlu Şengül,Pınarbaş Çetin Müberra,tarakçı cemre,Veyisoğlu Gökhan Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.4, no.3, 2019, ss.302 - 311.
AMA	BOZDEVECİ A,Alpay Karaoğlu Ş,Pınarbaş Çetin M,tarakçı c,Veyisoğlu G Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2019; 4(3): 302 - 311.
Vancouver	BOZDEVECİ A,Alpay Karaoğlu Ş,Pınarbaş Çetin M,tarakçı c,Veyisoğlu G Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2019; 4(3): 302 - 311.
IEEE	BOZDEVECİ A,Alpay Karaoğlu Ş,Pınarbaş Çetin M,tarakçı c,Veyisoğlu G "Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 4, ss.302 - 311, 2019.
ISNAD	BOZDEVECİ, ARİF vd. "Fasulye Gelişiminde, Rhizoctonia solani B227'nin Biyotik Stresine Karşı Trichoderma harzianum ID11C'nin Biyokontrol Etkinliği". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 4/3 (2019), 302-311.