TY  - JOUR
TI  - Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test
AB  - In this study, it was aimed at early detection of yersiniosis caused by Yersinia ruckeri in rainbow trouts (Oncorhynchusmykiss) using an agglutination test. O-antigen of Y. ruckeri isolated from diseased fishes was prepared. This prepared O-antigen was injectedinto the ear vein of the rabbit. Serum was removed from blood samples taken on the 7th and 15th days after the last injection. Thus, the antibodyof the Yersinia ruckeri was produced in the rabbit. Titers of the resulting antibodies were determined by microtitering in 96 well-based plates. Itwas used successfully in the slide an agglutination tests by making necessary dilutions according to the titer ratios of the antibodies. Wedetermined that the motile and Gr (-) bacteria isolated from diseased rainbow trout with yersiniozis symptom were Y. ruckeri according to theresults of slide agglutination test without any biochemical test. The isolated bacteria were confirmed to be Y. ruckeri with the results of API 20Etest kit and PCR test. The antibodies were stored at -20 and -80°C for use subsequent studies.
AU  - BALTA, FIKRI
AU  - DENGİZ BALTA, ZEYNEP
PY  - 2019
JO  - JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES
VL  - 4
IS  - 3
SN  - 2548-0006
SP  - 480
EP  - 483
DB  - TRDizin
UR  - http://search/yayin/detay/392824
ER  -