Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data
Yıl: 2020 Cilt: 5 Sayı: 2 Sayfa Aralığı: 241 - 244 Metin Dili: İngilizce DOI: 10.35229/jaes.738822 İndeks Tarihi: 01-02-2021
Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data
Öz: There is no study about the molecular characterization of the mitochondrial rrnS geneof Anisakis typica from the Turkish Mediterranean coasts. Thus, molecular characterization ofthe rrnS gene of A. typica from Turkish waters is aimed for the first time in the present study.Ascaridoid marine nematodes were collected and morphologically identified by light microscope.Identified Anisakis type I larvae were randomly selected and molecularly analysed by RestrictionFragment Length Polymorphism (RFLP) for the internal transcribed spacer (ITS) region. Anisakistypica larvae identified by PCR-RFLP analyses were amplified and sequenced with bothdirections for the rrnS gene. The rrnS sequence of A. typica from the Mediterranean coasts ofTurkey (MT395672) showed 99.80% identity (100% query coverage) with rrnS gene of A. typica(JX500052) from the Caribbean Sea, Atlantic Ocean. Between A. typica from the MediterraneanSea (MT395672) isolate and the Caribbean Sea isolate differed by only one nucleotide (C‒T, atalignment position 437). Pairwise genetic distance between the rrnS sequence of A. typica hereinand other A. typica from the Caribbean Sea was 0.002. Consequently, we determined themitochondrial rrnS data of A. typica from the Mediterranean Sea in the present study for the firsttime. The valid genetic data (MT395672) reported here can be used to molecular identification ofAnisakis species from the Mediterranean Sea and worldwide.
Anahtar Kelime: Türkiye'nin Akdeniz Kıyılarından Anisakis typica'nın (Nematoda: Anisakidae) Moleküler Karakterizasyonu: İlk Mitokondriyal rrnS Dizi Verileri
Öz: Türkiye Akdeniz kıyılarındaki Anisakis typica'nın mitokondriyal rrnS geninin moleküler karakterizasyonu hakkında bir çalışma bulunmamaktadır. Bu nedenle mevcut araştırmada Türk sularından izole edilen A. typica'nın rrnS geninin moleküler karakterizasyonu amaçlanmıştır. Ascaridoid deniz nematodları toplandı ve morfolojik olarak ışık mikroskobu ile teşhis edildi. Tanımlanan Anisakis tip I larvaları rastgele seçildi ve Sınırlayıcı Enzim Parça Uzunluk Çeşitliliği (RFLP) yöntemi ile internal transcribed spacer (ITS) bölgesi yönünden moleküler olarak analiz edildi. RFLP analizleri ile Anisakis typica olarak teşhis edilen larvaların rrnS geni çoğaltıldı ve iki yönlü DNA dizi analizleri yapıldı. Türkiye’nin Akdeniz kıyılarından elde edilen A. typica’nın rrnS geni (MT395672) Atlantik Okyanusu Karayip Denizinden elde edilen A. typica’nın rrnS geni (JX500052) ile %99,80 oranında (%100 sorgu kapsamı) benzerlik gösterdi. Akdeniz'den A. typica (MT395672) izolatı ile Karayip Denizi izolatı arasında sadece bir nükleotit (C ‒ T, hizalama pozisyonu 437) farklıydı. Buradaki A. typica'nın rrnS dizisi ile Karayip Denizi'nden diğer A. typica arasındaki ikili genetik mesafe 0.002'dir. Sonuç olarak bu çalışmada ilk kez Akdeniz'den A. typica'nın mitokondriyal rrnS verilerini belirledik. Burada bildirilen geçerli genetik veri (MT395672), Anisakis türlerinin Akdeniz'den ve dünyadan moleküler tanımlanması için kullanılabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410. DOI: 10.1016/S0022-2836(05)80360-2.
- Berland, B. (1961). Nematodes from some Norwegian marine fishes. Sarsia, 2, 1-50.
- D'Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanço, M. & Paggi, L. (2000). Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. International Journal for Parasitology, 30(2), 223-226. DOI: 10.1016/s0020-7519(99)00178-2.
- D'Amelio, S., Barros, N.B., Ingrosso, S., Fauquier, D.A., Russo, R. & Paggi L. (2007). Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species. Parasitology, 134, 1041-1051. DOI: 10.1017/S003118200700251X.
- Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P. & Drummond, A. (2012). Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647-1649.
- Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evoluation, 35, 1547- 1549. DOI: 10.1093/molbev/msy096.
- Mattiucci, S., Cipriani, P., Levsen, A., Paoletti, M. & Nascetti, G. (2018). Molecular epidemiology of Anisakis and Anisakiasis: an ecological and evolutionary road map. Advances in Parasitology, 9, 93-263. DOI: 10.1016/bs.apar.2017.12.001.
- Mattiucci, S. & Nascetti, G. (2008). Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite coevolutionary processes. Advances in Parasitology, 66, 47-148. DOI: 10.1016/S0065- 308X(08)00202-9.
- Mattiucci, S., Cipriani, P., Webb, S. C., Paoletti, M., Marcer, F., Bellisario, B., Gibson, D. & Nascetti, G. (2014). Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). Journal of Parasitology, 100, 199-214. DOI: 10.1645/12- 120.1.
- Pekmezci, G.Z. (2019). Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvae infecting Merlangius merlangus euxinus (Linnaeus, 1758) from the Turkish Black Sea coast based on mitochondrial small subunit ribosomal RNA gene analysis. Etlik Veteriner Mikrobiyoloji Dergisi, 30(1), 64-69. DOI: 10.35864/evmd.562427.
- Pekmezci, G.Z., Onuk, E.E., Bolukbas, C.S., Yardimci, B., Gurler, A.T., Acici, M. & Umur, S. (2014). Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Veterinary Parasitology, 201, 82-94. DOI: 10.1016/j.vetpar.2014.01.005.
- Pekmezci, G.Z. & Yardimci, B. (2019). On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters. Parasitology Research, 118(5), 1393-1402. DOI: 10.1007/s00436-019-06278-x.
- Peter, A.J. & Maillard, C. (1988). Larves d’ascarides parasites de poisons en Mediterranee occidentale. Bulletin du Museum National d'histoire Naturelle, 10 (sect. A), 347-369.
- Zhu, X., Gasser, R.B., Podolska, M, & Chilton, N.B. (1998). Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology, 28, 1911-1921. DOI: 10.1016/s0020-7519(98)00150-7.
| APA | Pekmezci G, ONUK E (2020). Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. , 241 - 244. 10.35229/jaes.738822 |
| Chicago | Pekmezci Gokmen Zafer,ONUK ERTAN EMEK Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. (2020): 241 - 244. 10.35229/jaes.738822 |
| MLA | Pekmezci Gokmen Zafer,ONUK ERTAN EMEK Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. , 2020, ss.241 - 244. 10.35229/jaes.738822 |
| AMA | Pekmezci G,ONUK E Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. . 2020; 241 - 244. 10.35229/jaes.738822 |
| Vancouver | Pekmezci G,ONUK E Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. . 2020; 241 - 244. 10.35229/jaes.738822 |
| IEEE | Pekmezci G,ONUK E "Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data." , ss.241 - 244, 2020. 10.35229/jaes.738822 |
| ISNAD | Pekmezci, Gokmen Zafer - ONUK, ERTAN EMEK. "Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data". (2020), 241-244. https://doi.org/10.35229/jaes.738822 |
| APA | Pekmezci G, ONUK E (2020). Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 5(2), 241 - 244. 10.35229/jaes.738822 |
| Chicago | Pekmezci Gokmen Zafer,ONUK ERTAN EMEK Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 5, no.2 (2020): 241 - 244. 10.35229/jaes.738822 |
| MLA | Pekmezci Gokmen Zafer,ONUK ERTAN EMEK Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, vol.5, no.2, 2020, ss.241 - 244. 10.35229/jaes.738822 |
| AMA | Pekmezci G,ONUK E Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2020; 5(2): 241 - 244. 10.35229/jaes.738822 |
| Vancouver | Pekmezci G,ONUK E Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data. JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES. 2020; 5(2): 241 - 244. 10.35229/jaes.738822 |
| IEEE | Pekmezci G,ONUK E "Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data." JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES, 5, ss.241 - 244, 2020. 10.35229/jaes.738822 |
| ISNAD | Pekmezci, Gokmen Zafer - ONUK, ERTAN EMEK. "Molecular Characterization of Anisakis typica (Nematoda: Anisakidae) from the Mediterranean Sea Coasts of Turkey: First Mitochondrial rrnS Sequence Data". JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES 5/2 (2020), 241-244. https://doi.org/10.35229/jaes.738822 |
