TY  - JOUR
TI  - CHANGES OCCURRING IN THE PROTEOME OF SH-SY5Y CELLS CAUSED BY FAT MASS AND OBESITY ASSOCIATED (FTO) PROTEIN EXPRESSION REVEALS MULTIFACETED PROPERTIES OF THE FTO PROTEIN
AB  - Objective: Fat mass and obesity associated (FTO) protein is an RNA-demethylase which is employed in various metabolic functions such as posttranscriptional modifications, DNA repair and fatty acid utilization. Fat mass and obesity associated protein was initially found to be closelyassociated with obesity and increased body-mass-index and later studies have established association of FTO with neurological diseases and cancer.The aim of this study was to investigate the effect of R316Q FTO mutation on soluble proteome in SH-SY5Y cells.Methods: SH-SY5Y cells stably expressing the wild-type (WT) and the mutant FTO proteins under the control of Tet-promoter were used to studychanges in overall proteome using two-dimentional Difference Gel Electrophoresis approach. More than 500 protein spots were compared in samplesthat overexpressed the WT-FTO or the mutant-FTO protein according to 2-fold-criteria. Spots displaying differences were cut from the gels andidentified by MALDI-TOF/TOF.Results: In overall, the expression of neither the WT nor the mutant FTO caused major changes in the soluble proteome. However, we observedsome minor changes in six protein spots. Three of those protein spots belonged to Hsp70 and were up-regulated in the mutant-FTO-expressing cells.This indicated that Hsp70 was not only up-regulated but also post-translationally modified. The other proteins regulated were phosphoglyceratekinase-1, calmodulin and keratin.Conclusion: These results indicated that FTO appear to be associated with energy metabolism and might induce the cellular stress. In addition, FTOmight affect to the Wnt signalling pathway. In overall, our study highlighted the multifaceted properties of the FTO and reflected onto the changesoccurring in the proteome of neuroblastoma cells.
AU  - KANLI, Aylin
AU  - Kasap, Murat
AU  - Akpınar, Gürler
AU  - Yanar, Sevinc
DO  - 10.30934/kusbed.666084
PY  - 2020
JO  - Kocaeli Üniversitesi Sağlık Bilimleri Dergisi
VL  - 6
IS  - 2
SN  - 2149-8571
SP  - 101
EP  - 112
DB  - TRDizin
UR  - http://search/yayin/detay/393515
ER  -