Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)
Yıl: 2018 Cilt: 11 Sayı: 4 Sayfa Aralığı: 430 - 433 Metin Dili: İngilizce DOI: 10.30607/kvj.449025 İndeks Tarihi: 03-02-2021
Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)
Öz: Canine demodicosis (CD) is an inflammatory skin disease caused by excessive proliferation of Demodex mites (Acarina:Demodicidae) former in the dogs’ follicles latter in the sebaceous glands. To date Demodex canis, D. injai, and D. cornei arethe three Demodex species that recognized in the domestic dog Canis familiaris. There are reports of morphologicalidentification of Demodex spp. from pet dogs in Turkey. Nevertheless there had been no reports of characterizing theDemodex mite from pet dogs in Turkey using well-defined mitochondrial DNA gene sequences. The aim of the presentstudy is to reveal first molecular characterization data on D. canis infesting domestic dogs (Canis familiaris) in Turkey.Demodex mites were obtained from skin scrapings of dogs with CD and identified microscopically as D. canis. Themitochondrial DNA 12S (mtDNA 12S) rRNA gene amplified, sequenced and compared with available Demodex sequencesin Genbank using BLAST analyses. D. canis isolate (DP-Samsun, MH374631) obtained in the study matched 100%(474/474) with previously reported gene sequences for the 12S rRNA of mtDNA in D. canis (KX264486, isolateDemo.can2) from dogs in China. Pairwise comparison between the 12S rRNA sequences of the D. canis from Turkey(MH374631) and other mtDNA rRNA gene of D. canis isolates from China (KX264486-88) presented differences rangingfrom 0.0 to 0.6 %. In conclusion, within the present study, we provided the molecular characterization of D. canis indomestic dogs for the first time by sequencing of the partial mtDNA in Turkey.
Anahtar Kelime: Evcil Köpeklerde (Canis Familiaris) Demodex Canis’in (Acarina: Demodicidae) Moleküler Karakterizasyonu
Öz: Kanin demodikozis (KD) Demodex akarlarının (Acarina: Demodicidae) kıl köklerinde ve yağ bezlerinde aşırı çoğalmasının neden olduğu inflamatuar bir deri hastalığıdır. Bugüne kadar evcil köpeklerde Canis familiaris bildirilen üç Demodex türü D. canis, D. injai ve D. cornei’dir. Türkiye’de pet köpeklerinde Demodex spp.’nin morfolojik identifikasyonu ile ilgili raporlar vardır. Buna rağmen Türkiye'de evcil köpeklerde Demodex akarlarının iyi tanımlanmış mitokondriyal DNA gen sekanslarının moleküler karakterizasyonu ile ilgili raporlar yoktur. Bu çalışmanın amacı Türkiye'de evcil köpeklerde (Canis familiaris) D. canis üzerine ilk moleküler karakterizasyon verilerini ortaya çıkarmaktır. Demodex akarları KD'li köpeklerin deri kazıntılarından elde edildi ve mikroskobik incelemeler ile D. canis olarak teşhis edildi. Mitokondriyal DNA’nın 12S rRNA geni çoğaltıldı, sekanslandı ve Genbank’ta BLAST analizleriyle mevcut bilinen Demodex sekansları ile karşılaştırıldı. Araştırmada elde edilen D. canis izolatı (DP-Samsun, MH374631) Çin’de daha önce köpeklerde rapor edilen D. canis 12S rRNA gen sekansı (KX264486, Demo.can2) ile % 100 (474/474) olarak eşleşti. Türkiye'den D. canis'in 12S rRNA dizisi (MH374631) ile Çin'den rapor edilmiş D. canis izolatlarının (KX264486-88) 12S rRNA sekansları arasındaki ikili hizalama analizleri % 0,0 ile % 0,6 arasında değişen farklılıkları ortaya çıkarmıştır. Sonuç olarak bu araştırma ile Türkiye’de ilk kez evcil köpeklerde D. canis türünün moleküler karakterizasyonu mitokondriyal DNA sekansları kullanarak sağlandı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Pekmezci G, PEKMEZCİ D, BÖLÜKBAŞI C (2018). Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). , 430 - 433. 10.30607/kvj.449025 |
| Chicago | Pekmezci Gokmen Zafer,PEKMEZCİ Didem,BÖLÜKBAŞI Cenk Soner Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). (2018): 430 - 433. 10.30607/kvj.449025 |
| MLA | Pekmezci Gokmen Zafer,PEKMEZCİ Didem,BÖLÜKBAŞI Cenk Soner Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). , 2018, ss.430 - 433. 10.30607/kvj.449025 |
| AMA | Pekmezci G,PEKMEZCİ D,BÖLÜKBAŞI C Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). . 2018; 430 - 433. 10.30607/kvj.449025 |
| Vancouver | Pekmezci G,PEKMEZCİ D,BÖLÜKBAŞI C Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). . 2018; 430 - 433. 10.30607/kvj.449025 |
| IEEE | Pekmezci G,PEKMEZCİ D,BÖLÜKBAŞI C "Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)." , ss.430 - 433, 2018. 10.30607/kvj.449025 |
| ISNAD | Pekmezci, Gokmen Zafer vd. "Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)". (2018), 430-433. https://doi.org/10.30607/kvj.449025 |
| APA | Pekmezci G, PEKMEZCİ D, BÖLÜKBAŞI C (2018). Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). Kocatepe Veterinary Journal, 11(4), 430 - 433. 10.30607/kvj.449025 |
| Chicago | Pekmezci Gokmen Zafer,PEKMEZCİ Didem,BÖLÜKBAŞI Cenk Soner Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). Kocatepe Veterinary Journal 11, no.4 (2018): 430 - 433. 10.30607/kvj.449025 |
| MLA | Pekmezci Gokmen Zafer,PEKMEZCİ Didem,BÖLÜKBAŞI Cenk Soner Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). Kocatepe Veterinary Journal, vol.11, no.4, 2018, ss.430 - 433. 10.30607/kvj.449025 |
| AMA | Pekmezci G,PEKMEZCİ D,BÖLÜKBAŞI C Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). Kocatepe Veterinary Journal. 2018; 11(4): 430 - 433. 10.30607/kvj.449025 |
| Vancouver | Pekmezci G,PEKMEZCİ D,BÖLÜKBAŞI C Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris). Kocatepe Veterinary Journal. 2018; 11(4): 430 - 433. 10.30607/kvj.449025 |
| IEEE | Pekmezci G,PEKMEZCİ D,BÖLÜKBAŞI C "Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)." Kocatepe Veterinary Journal, 11, ss.430 - 433, 2018. 10.30607/kvj.449025 |
| ISNAD | Pekmezci, Gokmen Zafer vd. "Molecular Characterization of Demodex Canis (Acarina: Demodicidae) in Domestic Dogs (Canis Familiaris)". Kocatepe Veterinary Journal 11/4 (2018), 430-433. https://doi.org/10.30607/kvj.449025 |
