Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation
Yıl: 2019 Cilt: 12 Sayı: 1 Sayfa Aralığı: 15 - 19 Metin Dili: İngilizce DOI: 10.30607/kvj.492556 İndeks Tarihi: 04-02-2021
Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation
Öz: The aim of this study was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ramsemen spermatological parameters on short term preservation at +4 ⁰C. Collected semen with electroejaculator atnon-breeding season. Each animal native ejaculates were mixed and divided into 4 equal groups, diluated withtris/egg yolk extender with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x106) and control(C). After extended semen samples were preservation at +4 ⁰C and evaluated motility, membrane integrity andabnormal spermatozoa rate until 96. hour. There were no significant differences between the groups on abnormalspermatozoa rate (P>0.05); there were a significant differences between the groups on motility at all storageperiod (P<0.05).The highest motility were observed in 7-DCLC 1.5 mg at 72. and 96. hour (P<0.05). The highestdose (5 mg/120x106) 7-DCLC were decreased the motility and membrane integrity compared to other groups.Besides, 7-DCLC 3.0 mg group membrane integrity was lower than control at 72. hour (P<0.05). In conclusion,tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membraneintegrity up to 96. hour at +4°C preservation.
Anahtar Kelime: Kısa Süreli Saklanan Koç Spermasında 7-Dehidrokolesterolün Spermatolojik Parametrelere Etkisi
Öz: Bu çalışmanın amacı, 7-dehidrokolesterol yüklenmiş siklodekstrinin (7-DCLC) +4 C⁰’de kısa süreli saklanan koç spermasının spermatolojik parametrelerine etkisinin araştırılmasıdır. Her bir hayvandan spermalar elektroejakülatör yardımı ile sezon dışında alınarak dört eşit gruba ayrıldı, farklı oranlarda 7-DCLC (1,5 mg, 3,0 mg and 5 mg/120x106 ) içeren ve içermeyen kontrol (C) tris/yumurta sarısı sulandırıcı ile sulandırıldı. Sulandırılan spermalar, +4 C⁰’de saklanarak 96 saat süresince motilite, membran bütünlüğü ve anormal spermatozoa yönünden incelendi. Saklama süresi boyunca anormal spermatozoa yönünden gruplar arasında bir farklılık bulunmazken (P>0,05); motilite açısından gruplar arasında farklılıklar tespit edildi (P<0,05). 72. ve 96. zaman dilimlerinde en yüksek motilite 7-DCLC 1,5 mg/120x106 grubunda tespit edildi (P<0,05). En yüksek dozlu (5 mg) 7-DCLC grubu diğer gruplarla karşılaştırıldığında spermatozoa motilitesini ve membran bütünlüğünü düşürdüğü tespit edildi. Ayrıca, 72. saatte 7-DCLC 3,0 mg grubunun membran bütünlüğü kontrol grubuna göre daha az olduğu tespit edildi (P<0,05). Sonuç olarak, 7-DCLC 1,5 mg içeren tris/yumurta sarısı sulandırıcısı koç spermasında motiliteyi ve membran bütünlüğünü +4 C⁰’de 96 saat süresince koruduğu belirlendi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | İNANÇ M, GÜNGÖR Ş, Ata A (2019). Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. , 15 - 19. 10.30607/kvj.492556 |
| Chicago | İNANÇ MUHAMMED ENES,GÜNGÖR Şükrü,Ata Ayhan Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. (2019): 15 - 19. 10.30607/kvj.492556 |
| MLA | İNANÇ MUHAMMED ENES,GÜNGÖR Şükrü,Ata Ayhan Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. , 2019, ss.15 - 19. 10.30607/kvj.492556 |
| AMA | İNANÇ M,GÜNGÖR Ş,Ata A Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. . 2019; 15 - 19. 10.30607/kvj.492556 |
| Vancouver | İNANÇ M,GÜNGÖR Ş,Ata A Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. . 2019; 15 - 19. 10.30607/kvj.492556 |
| IEEE | İNANÇ M,GÜNGÖR Ş,Ata A "Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation." , ss.15 - 19, 2019. 10.30607/kvj.492556 |
| ISNAD | İNANÇ, MUHAMMED ENES vd. "Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation". (2019), 15-19. https://doi.org/10.30607/kvj.492556 |
| APA | İNANÇ M, GÜNGÖR Ş, Ata A (2019). Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. Kocatepe Veterinary Journal, 12(1), 15 - 19. 10.30607/kvj.492556 |
| Chicago | İNANÇ MUHAMMED ENES,GÜNGÖR Şükrü,Ata Ayhan Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. Kocatepe Veterinary Journal 12, no.1 (2019): 15 - 19. 10.30607/kvj.492556 |
| MLA | İNANÇ MUHAMMED ENES,GÜNGÖR Şükrü,Ata Ayhan Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. Kocatepe Veterinary Journal, vol.12, no.1, 2019, ss.15 - 19. 10.30607/kvj.492556 |
| AMA | İNANÇ M,GÜNGÖR Ş,Ata A Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. Kocatepe Veterinary Journal. 2019; 12(1): 15 - 19. 10.30607/kvj.492556 |
| Vancouver | İNANÇ M,GÜNGÖR Ş,Ata A Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation. Kocatepe Veterinary Journal. 2019; 12(1): 15 - 19. 10.30607/kvj.492556 |
| IEEE | İNANÇ M,GÜNGÖR Ş,Ata A "Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation." Kocatepe Veterinary Journal, 12, ss.15 - 19, 2019. 10.30607/kvj.492556 |
| ISNAD | İNANÇ, MUHAMMED ENES vd. "Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation". Kocatepe Veterinary Journal 12/1 (2019), 15-19. https://doi.org/10.30607/kvj.492556 |
