Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats

Sefa KÜÇÜKLER, (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)
Selçuk ÖZDEMİR, (Atatürk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Erzurum, Türkiye)
Selim ÇOMAKLI, (Atatürk Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Erzurum, Türkiye)
Fatih Mehmet KANDEMİR (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiy)
Kocatepe Veterinary Journal
5 0
Yıl: 2020 Cilt: 13 Sayı: 2 Sayfa Aralığı: 161 - 171 Metin Dili: İngilizce DOI: 10.30607/kvj.709842 İndeks Tarihi: 08-02-2021

Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats

Öz:
The aim of the study was to investigate the effects of chrysin (CH), one of the natural flavonoids, againstisoniazid lung damage caused by isoniazid (INH), which was widely used in the treatment of tuberculosis. MaleSprague-Dawley rats were randomly divided into five groups: a control group, INH-treated group, CH alonetreated group 50 mg / kg, INH + CH 25 mg / kg treated group, and INH+ CH 50 mg / kg treated group. It wasdetermined that INH caused oxidative damage by decreasing antioxidant enzyme activities such as glutathioneperoxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) and increasing lipid peroxidation (LPO). Inaddition, it was found that the administration of CH to INH-treated rats increased GSH level and antioxidantenzyme activities, and decreased lipid peroxidation. It was observed that the nuclear factor erythroid 2 relatedfactor 2 (Nrf-2) and oxygenase-1 (HO-1) expression levels were up-regulated in the INH-treated group, and theexpression of NF-κB increased in the INH-treated group in the immunohistochemical examination, and the CHadministration, on the other hand, decreased the levels of these markers. Taken together, these results suggestedthat CH had beneficial effects in INH-induced lung toxicity by maintaining the oxidant-antioxidant balance anddecreasing NF-κB, Nrf-2, and HO-1 expressions.
Anahtar Kelime:

Ratlarda İzoniazid Kaynaklı Akciğer Hasarına Karşı Krisinin Etkileri

Öz:
Bu çalışmanın amacı; tüberküloz tedavisinde yaygın olarak kullanılan izoniazid (İZN) kaynaklı akciğer hasarına karşı doğal flavonoidlerden olan krisin (KRS)’in etkilerinin araştırılmasıdır. Çalışmada Spraque Dawley cinsi 35 adet erkek rat rastgele 5 gruba ayrıldı: Kontrol grubu, İZN uygulanan grup, KRS 50 mg/kg uygulanan grup, İZN+ KRS 25 mg/kg uygulanan grup ve İZN+ KRS 50 mg/kg uygulanan grup. İZN’nin glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) ve katalaz (KAT) gibi antioksidan enzim aktivitelerini ve glutatyon (GSH) düzeylerini azaltıp, lipid peroksidasyonunu (LPO) artırarak oksidatif hasara neden olduğu belirlendi. Ayrıca İZN ile kombine uygulanan KRS uygulamasının GSH seviyesini ve antioksidan enzim aktivitelerini artırdığı, lipid peroksidasyonunu ise azalttığı ettiği tespit edildi. Çalışmada incelenen nükleer faktör eritroid 2 ile ilişkili faktör 2 (Nrf-2) ve hem oksijenaz-1 (HO-1) seviyelerinin İZN grubunda gen ekspresyonu düzeyinde, nükleer faktör kappa B (NF-κB) ekspresyonunu ise immunhistokimyasal incelemede arttığı tespit edilmiş, buna karşın KRS uygulamasının bu belirteçlerin düzeylerinde azalmaya neden olduğu gözlenmiştir. Birlikte ele alındığında, bu sonuçlar KRS'in oksidan-antioksidan dengesini koruyarak ve NF-κB, Nrf-2 ve HO-1 ekspresyonlarını azaltarak İZN’nin neden olduğu akciğer toksisitesinde faydalı etkilere sahip olduğunu düşündürmektedir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KÜÇÜKLER S, özdemir s, ÇOMAKLI S, KANDEMİR F (2020). Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. , 161 - 171. 10.30607/kvj.709842
Chicago KÜÇÜKLER Sefa,özdemir selçuk,ÇOMAKLI Selim,KANDEMİR Fatih Mehmet Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. (2020): 161 - 171. 10.30607/kvj.709842
MLA KÜÇÜKLER Sefa,özdemir selçuk,ÇOMAKLI Selim,KANDEMİR Fatih Mehmet Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. , 2020, ss.161 - 171. 10.30607/kvj.709842
AMA KÜÇÜKLER S,özdemir s,ÇOMAKLI S,KANDEMİR F Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. . 2020; 161 - 171. 10.30607/kvj.709842
Vancouver KÜÇÜKLER S,özdemir s,ÇOMAKLI S,KANDEMİR F Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. . 2020; 161 - 171. 10.30607/kvj.709842
IEEE KÜÇÜKLER S,özdemir s,ÇOMAKLI S,KANDEMİR F "Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats." , ss.161 - 171, 2020. 10.30607/kvj.709842
ISNAD KÜÇÜKLER, Sefa vd. "Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats". (2020), 161-171. https://doi.org/10.30607/kvj.709842
APA KÜÇÜKLER S, özdemir s, ÇOMAKLI S, KANDEMİR F (2020). Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. Kocatepe Veterinary Journal, 13(2), 161 - 171. 10.30607/kvj.709842
Chicago KÜÇÜKLER Sefa,özdemir selçuk,ÇOMAKLI Selim,KANDEMİR Fatih Mehmet Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. Kocatepe Veterinary Journal 13, no.2 (2020): 161 - 171. 10.30607/kvj.709842
MLA KÜÇÜKLER Sefa,özdemir selçuk,ÇOMAKLI Selim,KANDEMİR Fatih Mehmet Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. Kocatepe Veterinary Journal, vol.13, no.2, 2020, ss.161 - 171. 10.30607/kvj.709842
AMA KÜÇÜKLER S,özdemir s,ÇOMAKLI S,KANDEMİR F Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. Kocatepe Veterinary Journal. 2020; 13(2): 161 - 171. 10.30607/kvj.709842
Vancouver KÜÇÜKLER S,özdemir s,ÇOMAKLI S,KANDEMİR F Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats. Kocatepe Veterinary Journal. 2020; 13(2): 161 - 171. 10.30607/kvj.709842
IEEE KÜÇÜKLER S,özdemir s,ÇOMAKLI S,KANDEMİR F "Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats." Kocatepe Veterinary Journal, 13, ss.161 - 171, 2020. 10.30607/kvj.709842
ISNAD KÜÇÜKLER, Sefa vd. "Effects of Chrysin Against Isoniazid-Induced Lung Injury in Rats". Kocatepe Veterinary Journal 13/2 (2020), 161-171. https://doi.org/10.30607/kvj.709842
Sayfa Oluşturulma Tarihi
23-04-2024 16:38

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