Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey

ÜSTÜNER, Hakan; ARSLAN ÖZDEMİR, Öznur; KANDAZOĞLU, Oğuz; Ardicli, Sena

Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey

Sena Ardıçlı, (Bursa Uludağ Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı)

Hakan Üstüner, (Bursa Uludağ Üniversitesi, Veteriner Fakültesi, Zootekni Bölümü)

Öznur ARSLAN ÖZDEMİR, (Bursa Uludağ Üniversitesi, Veteriner Fakültesi, Zootekni Bölümü)

Oğuz Kandazoğlu (Tabiat Tarım Limited Şirketi)

Lalahan Hayvancılık Araştırma Enstitüsü Dergisi

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Yıl: 2019 Cilt: 59 Sayı: 2 Sayfa Aralığı: 72 - 78 Metin Dili: İngilizce İndeks Tarihi: 03-03-2021

Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey

Öz:

The objective of the present study was to determine genotypic/allelic frequencies and population geneticindices of CAPN1 g.5709 C>G and MYF5 g.1911 A>G polymorphisms in beef cattle imported from Brazil to Turkey.Single nucleotide polymorphisms were carried out using the Polymerase Chain Reaction-Restriction Fragment LengthPolymorphism (PCR-RFLP) analysis. A total of 108 bulls, including Angus, Angus×Hereford×Nellore, Brahman,Hereford, Limousine, and Charolais breeds, were genotyped. Concerning the CAPN1, CC genotype was not found inthis study. Besides, the G allele frequency was quite high (0.75). Regarding the MYF5, frequency of AA genotype wasrather low (4.63%) compared to the other two genotypes, AG and GG. Therefore, the frequency of A allele was quitelow (0.21). On the basis of breed-specific evaluation, the highest frequency of GG genotype of the CAPN1 was found inBrahman breed (88.24%) whereas the highest frequency of heterozygous genotype was determined in Charolais breed(85.71%). The frequency of MYF5 GG genotype was found to be very high in Limousine breed (75.00%). Moreover,MYF5 AA genotype was absent in Angus, Brahman, Limousine, and Charolais breeds. The present study may be usefulfor further genetic analyses conducted on beef cattle imported into Turkey.

Anahtar Kelime:

Brezilya’dan Türkiye’ye İthal Edilen Besi Sığırlarında CAPN1 g.5709 C>G ve MYF5 g.1911 A>G Polimorfizmlerine Ait Varyasyonlar

Öz:

Sunulan bu çalışmanın amacı Brezilya’dan Türkiye’ye ithal edilmiş olan ticari besi sığırlarında, CAPN1 g.5709 C>G ve MYF5 g.1911 A>G polimorfizmlerine ait genotipik/allelik frekansların ve populasyon genetiği parametrelerinin belirlenmesidir. Tek nükleotit polimorfizmlerine ait genotiplendirme işlemi, Polimeraz Zincir Reaksiyonu-Restriksiyon Parça Uzunluğu Polimorfizmi (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism: PCR–RFLP) analizi kullanılarak gerçekleştirilmiştir. Bu bağlamda, Angus, Angus×Hereford×Nellore, Brahman, Hereford, Limuzin ve Şarole ırklarını içeren toplam 108 baş erkek sığır genotiplendirilmiştir. Bu çalışmada, CAPN1 ile ilgili olarak CC genotipinin bulunmadığı görülmüştür. Ayrıca, G allel frekansının oldukça yüksek olduğu belirlenmiştir (0,75). MYF5 için AA genotip frekansının (%4,63) AG ve GG olan diğer iki genotip ile karşılaştırıldığında oldukça düşük olduğu ve dolayısıyla A allel frekansının (0,21) da düşük olduğu görülmüştür. Irk özelinde yapılan değerlendirmede, en yüksek CAPN1 GG genotip frekansı Brahman ırkında (%88,24) bulunurken; en yüksek heterozigot genotip frekansı ise Şarole ırkı sığırlarda (%85,71) belirlenmiştir. MYF5 GG genotip frekansının Limuzin ırkında çok yüksek olduğu (%75,00) görülmüştür. Buna ek olarak, MYF5 AA genotipinin Angus, Brahman, Limuzin ve Şarole ırklarında bulunmadığı belirlenmiştir. Bu çalışma, Türkiye’ye ithal edilen besi sığırları üzerinde ileride yapılacak genetik analizlere faydalı olabilecektir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Ardicli S, ÜSTÜNER H, ARSLAN ÖZDEMİR Ö, KANDAZOĞLU O (2019). Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. , 72 - 78.
Chicago	Ardicli Sena,ÜSTÜNER Hakan,ARSLAN ÖZDEMİR Öznur,KANDAZOĞLU Oğuz Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. (2019): 72 - 78.
MLA	Ardicli Sena,ÜSTÜNER Hakan,ARSLAN ÖZDEMİR Öznur,KANDAZOĞLU Oğuz Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. , 2019, ss.72 - 78.
AMA	Ardicli S,ÜSTÜNER H,ARSLAN ÖZDEMİR Ö,KANDAZOĞLU O Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. . 2019; 72 - 78.
Vancouver	Ardicli S,ÜSTÜNER H,ARSLAN ÖZDEMİR Ö,KANDAZOĞLU O Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. . 2019; 72 - 78.
IEEE	Ardicli S,ÜSTÜNER H,ARSLAN ÖZDEMİR Ö,KANDAZOĞLU O "Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey." , ss.72 - 78, 2019.
ISNAD	Ardicli, Sena vd. "Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey". (2019), 72-78.

APA	Ardicli S, ÜSTÜNER H, ARSLAN ÖZDEMİR Ö, KANDAZOĞLU O (2019). Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 59(2), 72 - 78.
Chicago	Ardicli Sena,ÜSTÜNER Hakan,ARSLAN ÖZDEMİR Öznur,KANDAZOĞLU Oğuz Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi 59, no.2 (2019): 72 - 78.
MLA	Ardicli Sena,ÜSTÜNER Hakan,ARSLAN ÖZDEMİR Öznur,KANDAZOĞLU Oğuz Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, vol.59, no.2, 2019, ss.72 - 78.
AMA	Ardicli S,ÜSTÜNER H,ARSLAN ÖZDEMİR Ö,KANDAZOĞLU O Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi. 2019; 59(2): 72 - 78.
Vancouver	Ardicli S,ÜSTÜNER H,ARSLAN ÖZDEMİR Ö,KANDAZOĞLU O Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi. 2019; 59(2): 72 - 78.
IEEE	Ardicli S,ÜSTÜNER H,ARSLAN ÖZDEMİR Ö,KANDAZOĞLU O "Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey." Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 59, ss.72 - 78, 2019.
ISNAD	Ardicli, Sena vd. "Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey". Lalahan Hayvancılık Araştırma Enstitüsü Dergisi 59/2 (2019), 72-78.