TY  - JOUR
TI  - Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations
AB  - Nerve Growth Factor Gene (NGF) has important functions in the maintenance of viability andproliferation of ovarian cells. Recent studies on caprine NGF gene revealed two SNPs(determined by SacII/C291A and MboII/A705G polymorphisms) related with body length andlitter size in goats. This study aimed to determine SacII and MboII polymorphisms of NGF innative Turkish goat populations including Turkish Hair (HAI), Kabakulak (KBK), Honamlı(HNM) and Norduz (NRD) by using PCR-RFLP. Amplificated PCR fragments of 808 weredigested with SacII, while amplificated PCR fragments of 319 bp were digested with MboIIrestriction enzyme in order to detect different genotypes of NGF gene. The “A” allelefrequency ranged from 0.500 (HAI, HNM and KBK) to 0.950 (NRD), while the “C” allelefrequency ranged from 0.025 (NRD) to 0.500 (HAI, HNM and KBK) in NGF/SacIIpolymorphism. The “A” allele frequency ranged from 0.213 (HAI) to 1.000 (NRD), while the“G” allele frequency ranged from 0.000 (NRD) to 0.787 (HAI) in NGF/MboII polymorphism.Deviation from HW equilibrium was significant in HNM goat population (P < 0.05). In thisstudy, polymorphisms of caprine NGF gene in native Turkish goat populations were revealedfor the first time. The results obtained from this study showed that NGF/SacII polymorphismscould be used for body length in NRD population while NGF/MboII polymorphisms could beused for litter size in HAI, HNM and KBK populations in MAS studies.
AU  - ARGUN KARSLI, Bahar
AU  - DEMİR, Eymen
AU  - Balcıoğlu, Murat Soner
AU  - Karslı, Taki
DO  - 10.29136/mediterranean.633476
PY  - 2020
JO  - Mediterranean  Agricultural Sciences
VL  - 33
IS  - 1
SN  - 2528-9675
SP  - 145
EP  - 148
DB  - TRDizin
UR  - http://search/yayin/detay/397713
ER  -