CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population

TAGA, Yavuz; KURT, Elif; TOPLAYICI, Selina; OKAT, Zehra; ÇİFTÇİ, Kezban UÇAR; YAMA, Kübra

doi:10.5472/marumj.430790

CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population

Zehra OKAT, (Marmara Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Maltepe, 34854 İstanbul, Türkiye)

Kezban UÇAR ÇİFTÇİ, (Marmara Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Maltepe, 34854 İstanbul, Türkiye)

Kübra YAMA, (Marmara Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Maltepe, 34854 İstanbul, Türkiye)

Selina TOPLAYICI, (Elif KURT)

Elif KURT, (Marmara Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Maltepe, 34854 İstanbul, Türkiye)

Yavuz TAGA (Marmara Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Maltepe, 34854 İstanbul, Türkiye)

Marmara Medical Journal

2 0

Yıl: 2018 Cilt: 31 Sayı: 2 Sayfa Aralığı: 61 - 67 Metin Dili: İngilizce DOI: 10.5472/marumj.430790 İndeks Tarihi: 22-03-2021

CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population

Öz:

Objective: Many studies have shown the association of susceptibility to several cancers with gene encoding enzymes’ polymorphisms which engage in xenobiotics’ biotransformation. In this study, the main purpose is to search the relation between cytochrome P450 (CYP) 2D6* 4 polymorphisms and Polycythemia vera (PV) incidence in Turkish population.Materials and Methods: In this research article, 80 Polycthemia vera (PV) cases and 76 control samples have been used for the analysis of CYP 2D6* 4 polymorphism. The research has been performed by the methods of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).Results: As a result, when patients and controls were compared in terms of CYP 2D6* 4 poor metabolizer (PM) and heterozygous extensive metabolizer (HEM) genotype frequency, it has been found that the patients have 1.35% PM and 32.43% HEM frequency, and controls have 2.63% PM and 21.05% HEM frequency. The correlation between the results of CYP 2D6 genotype analysis and the risk of disease in patients and controls was examined. We found that PM, HEM and extensive metabolizer (EM) genotypes were unrelated with the risk of PV (OR 0.51, 95% CI 0.04-5.71, OR 1.80, 95% CI 0.86-3.75, χ2 1.93, P: 0.164, OR 0.61, 95% CI 0.30-1.24, χ2 1.40, P: 0.235).Conclusion: Our results suggested that the CYP 2D6* 4 polymorphism, in the studied population, does not play an important role in PV etiology.

Anahtar Kelime:

Türk popülasyonunda Polisitemia vera hastalarında CYP 2D6*4 polimorfizmi

Öz:

Amaç: Birçok çalışma, ksenobiyotiklerin biyotransformasyonundan sorumlu enzimleri kodlayan gen polimorfizmleri ile çeşitli kanserlere olan duyarlılık arasında bir ilişkili olduğunu göstermiştir. Bu çalışmada temel amaç, Türk popülasyonunda sitokrom P450 (CYP) 2D6*4 polimorfizmleri ile PV insidansı arasındaki ilişkiyi saptamaktır. Gereç ve Yöntem: Bu araştırma makalesinde, CYP 2D6* 4 polimorfizminin analizi için 80 (PV) hastası ve 76 kontrol örneği kullanılmıştır. Deney, polimerize zincir reaksiyonu (PZR)-restriksiyon parça uzunluğu polimorfizmi (RFLP) methodu kullanılarak gerçekleştirilmiştir. Bulgular: Sonuç olarak, hastalar ve kontroller CYP 2D6* 4 yavaş metabolizör (YM) ve heterozigot hızlı metabolizör (HM) genotip sıklığına göre karşılaştırıldıklarında, hastalardaki YM frekansı %1.35, heterozigot HM frekansı %32.43, kontrol grubunda ise YM frekansı %2.63, heterozigot HM frekansı %21.05 olarak tespit edilmiştir. CYP 2D6 genotiplerinin analiz sonuçları ile hasta ve kontrollerdeki hastalık riski arasındaki korelasyon araştırılmıştır. YM, heterozigot HM ve HM genotiplerinin PV riski ile ilişkili olmadığı bulunmuştur (OR 0.51, 95% CI 0.04-5.71, OR 1.80, 95% CI 0.86-3.75, χ2 1.93, P: 0.164, OR 0.61, 95% CI 0.30- 1.24, χ2 1.40, P: 0.235). Sonuç: Elde ettiğimiz sonuçlar, çalışılan popülasyonda CYP 2D6 * 4 polimorfizminin PV etyolojisinde önemli bir rol oynamadığını göstermiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	OKAT Z, ÇİFTÇİ K, YAMA K, TOPLAYICI S, KURT E, TAGA Y (2018). CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. , 61 - 67. 10.5472/marumj.430790
Chicago	OKAT Zehra,ÇİFTÇİ Kezban UÇAR,YAMA Kübra,TOPLAYICI Selina,KURT Elif,TAGA Yavuz CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. (2018): 61 - 67. 10.5472/marumj.430790
MLA	OKAT Zehra,ÇİFTÇİ Kezban UÇAR,YAMA Kübra,TOPLAYICI Selina,KURT Elif,TAGA Yavuz CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. , 2018, ss.61 - 67. 10.5472/marumj.430790
AMA	OKAT Z,ÇİFTÇİ K,YAMA K,TOPLAYICI S,KURT E,TAGA Y CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. . 2018; 61 - 67. 10.5472/marumj.430790
Vancouver	OKAT Z,ÇİFTÇİ K,YAMA K,TOPLAYICI S,KURT E,TAGA Y CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. . 2018; 61 - 67. 10.5472/marumj.430790
IEEE	OKAT Z,ÇİFTÇİ K,YAMA K,TOPLAYICI S,KURT E,TAGA Y "CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population." , ss.61 - 67, 2018. 10.5472/marumj.430790
ISNAD	OKAT, Zehra vd. "CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population". (2018), 61-67. https://doi.org/10.5472/marumj.430790

APA	OKAT Z, ÇİFTÇİ K, YAMA K, TOPLAYICI S, KURT E, TAGA Y (2018). CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. Marmara Medical Journal, 31(2), 61 - 67. 10.5472/marumj.430790
Chicago	OKAT Zehra,ÇİFTÇİ Kezban UÇAR,YAMA Kübra,TOPLAYICI Selina,KURT Elif,TAGA Yavuz CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. Marmara Medical Journal 31, no.2 (2018): 61 - 67. 10.5472/marumj.430790
MLA	OKAT Zehra,ÇİFTÇİ Kezban UÇAR,YAMA Kübra,TOPLAYICI Selina,KURT Elif,TAGA Yavuz CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. Marmara Medical Journal, vol.31, no.2, 2018, ss.61 - 67. 10.5472/marumj.430790
AMA	OKAT Z,ÇİFTÇİ K,YAMA K,TOPLAYICI S,KURT E,TAGA Y CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. Marmara Medical Journal. 2018; 31(2): 61 - 67. 10.5472/marumj.430790
Vancouver	OKAT Z,ÇİFTÇİ K,YAMA K,TOPLAYICI S,KURT E,TAGA Y CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population. Marmara Medical Journal. 2018; 31(2): 61 - 67. 10.5472/marumj.430790
IEEE	OKAT Z,ÇİFTÇİ K,YAMA K,TOPLAYICI S,KURT E,TAGA Y "CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population." Marmara Medical Journal, 31, ss.61 - 67, 2018. 10.5472/marumj.430790
ISNAD	OKAT, Zehra vd. "CYP 2D6* 4 polymorphism in Polycythemia vera patients in Turkish population". Marmara Medical Journal 31/2 (2018), 61-67. https://doi.org/10.5472/marumj.430790