Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi

Kaleli, Prof. Dr. Suleyman; DEVECİ ÖZKAN, Asuman; guney eskiler, gamze

Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi

Gamze Güney Eskiler, (Sakarya Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Sakarya, Türkiye)

Asuman Deveci Özkan, (Sakarya Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Sakarya, Türkiye)

Süleyman Kaleli (Sakarya Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Sakarya, Türkiye)

Sakarya Tıp Dergisi

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Yıl: 2018 Cilt: 8 Sayı: 4 Sayfa Aralığı: 766 - 774 Metin Dili: Türkçe İndeks Tarihi: 21-04-2021

Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi

Öz:

Amaç Nobiletin, C21H22O8 kimyasal formülü ve 402.39 kD molekül ağırlığına sahip, turunçgil kabuklarında bulunan bir O-metillenmişfl avanoiddir. Nobiletinin sahip olduğu antikanser, antiviral ve anti-infl amatuar etkilerden dolayı, kanser tedavisinde potansiyel terapötik birajan olarak dikkat çekmektedir. Prostat kanserinde metastaza neden olan kanser hücrelerinin invaziv özelliğinin yüksek olması, hastalığıntedavisini zorlaştırmakta ve hastalarda kötü prognoza neden olan en önemli faktörlerden birisidir. Bu nedenle mevcut çalışmada, nobiletininyüksek metastatik potansiyeli olan insan prostat kanseri (PC-3) ve insan göbek kordonu veni endotel hücrelerinde (HUVEC) sitotoksik veapoptotik etkilerinin belirlenmesi amaçlanmıştır. ( Sakarya Tıp Dergisi 2018, 8(4):766-774 )Gereç veYöntemlerMevcut çalışmada, PC-3 ve HUVEC hücrelerinde nobiletinin sitotoksik etkisi WST-1 analizi ile, apoptotik etkisi ise Annexin V, akridin oranjboyaması ve hücre siklusu analizleri ile kalitatif ve kantitatif olarak belirlenmiştir.Bulgular WST-1 analizi ile elde edilen bulgulara göre, 24 ve 48 saat boyunca farklı konsantrasyonlarda nobiletin uygulanan PC-3 hücrelerindecanlılık oranının anlamlı bir şekilde azaldığı belirlenmiştir. Annexin V ve hücre siklusu analizleri ile elde edilen verilere göre, nobiletininPC-3 hücrelerinde özellikle erken apoptoza ve G0/G1 fazında artışa neden olduğu belirlenmiştir. Ayrıca, HUVEC hücrelerinde 80 µMkonsantrasyona kadar toksik etkisinin olmadığı belirlenmiştir.Sonuç Kontrol hücreleri ile karşılaştırmalı olarak nobiletinin metastatik prostat kanseri hücrelerinde anti-proliferatif etkisinin, erken apoptoz ve G0/G1 fazında hücre miktarında artış ile gerçekleştiği gösterilmiştir. Ancak, nobiletinin metastatik prostat kanserinde neden olduğu apoptotikölümün moleküler mekanizmasının in vitro ve in vivo olarak detaylı araştırılması gerekmektedir.

Anahtar Kelime:

Determination of Cytotoxic and Apoptotic Effects of Nobiletin on Metastatic Prostate Cancer

Öz:

Objective Nobiletin is an O-methylated fl avanoid found in citrus with a C21H22O8 chemical formula and 402.39 kD molecular weight. Nobiletin has a potential therapeutic agent in the treatment of cancer due to the anticancer, antiviral and anti-infl ammatory effects. The high invasive property of cancer cells leading to metastasis, has complicated prostate cancer treatment and this is one of the most important factor causing poor prognosis in the patients with prostate cancer. Therefore, the aim of this study was to investigate the cytotoxic and apoptotic effects of nobiletin in high metastatic potential human prostate cancer cell line (PC-3) and human umbilical vein endothelial cells (HUVEC). ( Sakarya Med J 2018, 8(4):766-774 ). Materials and Methods In the present study, the cytotoxic effect of nobiletin was determined by WST-1 analysis, and the apoptotic effect was determined qualitatively and quantitatively by Annexin V, acridine orange staining and cell cycle analysis in PC-3 and HUVEC cells. Results According to the results obtained by WST-1 analysis, the viability of PC-3 cells signifi cantly reduced after treatment with different concentrations of nobiletin for 24 and 48 hours. According to the results obtained by Annexin V and cell cycle analysis, it was determined that nobiletin induced especially early apoptotic cell population and increased the proportion of PC-3 cell in the G0/G1 phase. Additionally, no toxic effects were observed up to 80 µM concentration of nobiletin in HUVEC cells. Conclusion Nobiletin has been shown to exert anti-proliferative effect through early apoptosis and increased number of cells arrested in G0/G1 phase in metastatic prostate cancer cells compared with control cell. However, the molecular mechanism of apoptotic cell death caused by nobiletin in metastatic prostate cancer should be investigated by in vitro and in vivo.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	guney eskiler g, DEVECİ ÖZKAN A, Kaleli P (2018). Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. , 766 - 774.
Chicago	guney eskiler gamze,DEVECİ ÖZKAN Asuman,Kaleli Prof. Dr. Suleyman Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. (2018): 766 - 774.
MLA	guney eskiler gamze,DEVECİ ÖZKAN Asuman,Kaleli Prof. Dr. Suleyman Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. , 2018, ss.766 - 774.
AMA	guney eskiler g,DEVECİ ÖZKAN A,Kaleli P Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. . 2018; 766 - 774.
Vancouver	guney eskiler g,DEVECİ ÖZKAN A,Kaleli P Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. . 2018; 766 - 774.
IEEE	guney eskiler g,DEVECİ ÖZKAN A,Kaleli P "Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi." , ss.766 - 774, 2018.
ISNAD	guney eskiler, gamze vd. "Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi". (2018), 766-774.

APA	guney eskiler g, DEVECİ ÖZKAN A, Kaleli P (2018). Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. Sakarya Tıp Dergisi, 8(4), 766 - 774.
Chicago	guney eskiler gamze,DEVECİ ÖZKAN Asuman,Kaleli Prof. Dr. Suleyman Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. Sakarya Tıp Dergisi 8, no.4 (2018): 766 - 774.
MLA	guney eskiler gamze,DEVECİ ÖZKAN Asuman,Kaleli Prof. Dr. Suleyman Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. Sakarya Tıp Dergisi, vol.8, no.4, 2018, ss.766 - 774.
AMA	guney eskiler g,DEVECİ ÖZKAN A,Kaleli P Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. Sakarya Tıp Dergisi. 2018; 8(4): 766 - 774.
Vancouver	guney eskiler g,DEVECİ ÖZKAN A,Kaleli P Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi. Sakarya Tıp Dergisi. 2018; 8(4): 766 - 774.
IEEE	guney eskiler g,DEVECİ ÖZKAN A,Kaleli P "Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi." Sakarya Tıp Dergisi, 8, ss.766 - 774, 2018.
ISNAD	guney eskiler, gamze vd. "Metastatik Prostat Kanserinde Nobiletinin Sitotoksik ve Apoptotik Etkisinin Belirlenmesi". Sakarya Tıp Dergisi 8/4 (2018), 766-774.