Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts
Yıl: 2019 Cilt: 43 Sayı: 4 Sayfa Aralığı: 429 - 440 Metin Dili: İngilizce DOI: 10.16970/entoted.539140 İndeks Tarihi: 28-04-2021
Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts
Öz: Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) causes economic losses in apple, pear, plum, willow, oak and other economically important trees. In this study, an Alphabaculovirus was isolated from the larval population of M. neustria from Samsun in the central Black Sea Region of Turkey between 2015 and 2016. Electron microscope analysis of occlusion bodies (OBs) obtained from dead larvae showed that the nucleocapsids of a new isolate (ManeNPV-T4) are multiply enveloped. The Kimura two-parameter analysis and the phylogenetic tree were performed based on concatenated nucleotide and amino acid sequences of the partial lef-8, lef-9 and polh genes from ManeNPV-T4 isolate compared to those of other 51 baculoviruses. Insecticidal activity tests against third instar M. neustria larvae produced 48 to 100% mortalities. The LC50 of ManeNPV-T4 was 0.78 x 103 OBs/ml in M. neustria. Additionally, the isolate caused mortalities lower than 50% in Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) and Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) larvae. Consequently, the new nucleopolyhedrovirus is infectious on M. neustria larvae and other lepidopterans.
Anahtar Kelime: Samsun’da Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae)'dan yeni bir bakülovirüs izolatının ve farklı konukçularda patojenitesinin belirlenmesi
Öz: Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae), Avrupa, Asya ve Kuzey Afrika'yadağılmış dünya çapında bir zararlıdır. Elma, armut, erik, söğüt ve meşede ekonomik kayıplara neden olur. Buçalışmada, Türkiye'nin Orta Karadeniz Bölgesi’nde bulunan Samsun'dan 2015-2016 yıllarında toplanan M. neustria larvalarından bir Alphabakülovirüs izole edilmiştir. Ölü larvalardan elde edilen oklüzyon badilerin (OB) elektronmikroskobu analizi, çoklu nükleokapsidlere sahip yeni bir izolat (ManeNPV-T4) olduğunu gösterdi. Kimura iki parametre analizi ve filogenetik ağaç, ManeNPV-T4 izolatından elde edilen kısmi lef-8, lef-9 ve polh genlerininbirleştirilmiş nükleotit ve amino asit dizilerine dayanarak, diğer 51 bakülovirüsünki ile karşılaştırıldı. Üçüncü evre M.neustria larvalarına uygulanan insektisidal aktivite testleri sonucunda %48 ile %100 arasında ölüm gözlendi.ManeNPV-T4'ün M. neustria için LC50 değeri 0,78 x 103 OB/ml olarak belirlendi. Ek olarak, viral izolat Spodopteraexigua (Hübner, 1808) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae),Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) ve Helicoverpa armigera (Hübner, 1805) (Lepidoptera:Noctuidae) larvaları üzerinde %50’den düşük ölüm oranı oluşturdu. Sonuç olarak, bu yeni nükleopolihedrovirüs M.neustria ve diğer lepidopter larvalarına karşı da enfektiviteye sahiptir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | GENÇER D, YANAR O, YEŞİLYURT A, NALÇACIOĞLU R, DEMİR İ (2019). Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. , 429 - 440. 10.16970/entoted.539140 |
| Chicago | GENÇER Dönüş,YANAR Oğuzhan,YEŞİLYURT Aydın,NALÇACIOĞLU Remziye,DEMİR İsmail Deniz Kağan Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. (2019): 429 - 440. 10.16970/entoted.539140 |
| MLA | GENÇER Dönüş,YANAR Oğuzhan,YEŞİLYURT Aydın,NALÇACIOĞLU Remziye,DEMİR İsmail Deniz Kağan Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. , 2019, ss.429 - 440. 10.16970/entoted.539140 |
| AMA | GENÇER D,YANAR O,YEŞİLYURT A,NALÇACIOĞLU R,DEMİR İ Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. . 2019; 429 - 440. 10.16970/entoted.539140 |
| Vancouver | GENÇER D,YANAR O,YEŞİLYURT A,NALÇACIOĞLU R,DEMİR İ Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. . 2019; 429 - 440. 10.16970/entoted.539140 |
| IEEE | GENÇER D,YANAR O,YEŞİLYURT A,NALÇACIOĞLU R,DEMİR İ "Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts." , ss.429 - 440, 2019. 10.16970/entoted.539140 |
| ISNAD | GENÇER, Dönüş vd. "Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts". (2019), 429-440. https://doi.org/10.16970/entoted.539140 |
| APA | GENÇER D, YANAR O, YEŞİLYURT A, NALÇACIOĞLU R, DEMİR İ (2019). Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. Türkiye Entomoloji Dergisi, 43(4), 429 - 440. 10.16970/entoted.539140 |
| Chicago | GENÇER Dönüş,YANAR Oğuzhan,YEŞİLYURT Aydın,NALÇACIOĞLU Remziye,DEMİR İsmail Deniz Kağan Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. Türkiye Entomoloji Dergisi 43, no.4 (2019): 429 - 440. 10.16970/entoted.539140 |
| MLA | GENÇER Dönüş,YANAR Oğuzhan,YEŞİLYURT Aydın,NALÇACIOĞLU Remziye,DEMİR İsmail Deniz Kağan Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. Türkiye Entomoloji Dergisi, vol.43, no.4, 2019, ss.429 - 440. 10.16970/entoted.539140 |
| AMA | GENÇER D,YANAR O,YEŞİLYURT A,NALÇACIOĞLU R,DEMİR İ Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. Türkiye Entomoloji Dergisi. 2019; 43(4): 429 - 440. 10.16970/entoted.539140 |
| Vancouver | GENÇER D,YANAR O,YEŞİLYURT A,NALÇACIOĞLU R,DEMİR İ Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts. Türkiye Entomoloji Dergisi. 2019; 43(4): 429 - 440. 10.16970/entoted.539140 |
| IEEE | GENÇER D,YANAR O,YEŞİLYURT A,NALÇACIOĞLU R,DEMİR İ "Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts." Türkiye Entomoloji Dergisi, 43, ss.429 - 440, 2019. 10.16970/entoted.539140 |
| ISNAD | GENÇER, Dönüş vd. "Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts". Türkiye Entomoloji Dergisi 43/4 (2019), 429-440. https://doi.org/10.16970/entoted.539140 |
