Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep
Yıl: 2018 Cilt: 37 Sayı: 2 Sayfa Aralığı: 87 - 91 Metin Dili: İngilizce DOI: 10.30782/uluvfd.413857 İndeks Tarihi: 28-04-2021
Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep
Öz: Ovulation rate and litter size, varies between breeds or individulas who belong to the same breed, are evaluated as fertility traits. Fertility traits are important for economic values in animal breeding. However these traits exhibit only in females and they are determined in the later stages of lifes. Hence the genetic perspective is more effective way to confirm the fertility capacities early period of life time. Fecundity genes such as BMP15 regulate the fertility traits by increasing the ovulation rate due to the occuring mutations. Despite some prolific breed were investigated for $FecX^B$ mutation; the researches carried out to determine that mutation in Chios sheep breed are limited. The objective of the current study was to estimate the the genotype frequencies of $FecX^B$ mutation of BMP15 gene in prolific Chios sheep. We investigated the $FecX^B$ mutation by PCR-RFLP method in Chios sheep (n=77). According to result, the investigated Chios breed was found monomorphic for $FecX^B$ mutation. All individuals were digested by DdeI restriction enzyme and showed wild-type genotype and did not carry $FecX^B$ mutation nin present study. In conclusion it’s thought that the high prolificacy in Chios sheep may be based on another region of BMP15 gene or differ-ent major gene. Thus the effect of other major genes or regions/QTL’s should be investigated in future studies with a large number of animals.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Barakat IAH, Salem LM, Daoud NM, Khalil WKB, Mahrous KF. Genetic polymorphism of candidate genes for fecundity traits in Egyptian sheep breeds. Biomed Res, 28 (2): 851-857, 2017.
- Bodin L, Di Pasquale E, Fabre S, Bontoux M, Monget P, Persani L, Mulsant P. A Novel Mutation in the Bone Mor-phogenetic Protein 15 Gene Causing Defective Protein Se-cretion Is Associated with Both Increased Ovulation Rate and Sterility in Lacaune Sheep. Endocrinology, 148(1): 393–40, 2007.
- Davis GH. Major genes affecting ovulation rate in sheep. Genet Sel Evol, 37(1):11–23, 2005.
- Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persan L, Tosser-Klopp G, Mulsant P, Nowak Z, Drobik W, Martyniuk E, Bodin L. Genome-Wide Association Studies Identify Two Novel BMP15 Mutations Responsible for an Atypical Hyperprolificacy Phenotype in Sheep. PLoS Genetics, 9(4): e1003482, 2013.
- Dinçel D, Ardıçlı S, Soyudal B, Er M, Alpay F, Şamlı H, Balcı F. Analysis of FecB, BMP15 and CAST gene mutations in Sakiz sheep. Kafkas Univ Vet Fak Derg, 21(4): 483-488, 2015.
- Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 19:11- 15, 1987.
- Drouilhet L, Lecerf F, Bodin L, Fabre S, Mulsant P. Fine mapping of the FecL locus influencing prolificacy in Lacaune sheep. Anim Genet, 40: 804–812, 2009.
- Esen Gürsel F, Akış I, Durak H, Mengi A, Öztabak K. Determination of BMP-15, BMPR-1B and GDF-9 Gene Mutations of the Indigenous Sheep Breeds in Turkey. Kafkas Univ Vet Fak Derg, 17(5): 725-729, 2011.
- Fabre S, Pierre A, Mulsant P, Bodin L, Di Papsquale E, Persani L, Monget P, Monniaux, D. Regulation of ovulation rate in mammals: contribution of sheep genetic models. Repro Biol Endoc, 4:20, 2006.
- Hanrahan JP, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R, Galloway SM. Mutations in the Genes for Oocyte-Derived Growth Factors GDF9 and BMP15 Are Associated with Both Increased Ovulation Rate and Sterility in Cambridge and Belclare Sheep (Ovis aries), Biol Repro, 70:900–909, 2004.
- Hanrahan JP. Evidence for single gene effects on ovulation rate in the Cambridge and Belclare breeds, In: Elsen J.M., Bodin L., Thimonier J. (Eds.). Major Genes for Reproduction in Sheep. Inra, Paris, France, pp. 93–102, 1991.
- Hatziminaoglu J, Zervas NP, Boyazoglu J. Prolific dairy sheep breeds in Greece. In : Bougler J.(Eds.), Tisserand J.- L. (Eds.). Les petits ruminants et leurs productions laitières dans la région méditerranéenne. Montpellier:CIHEAM, pp 25-30, 1990.
- Jansson T. Genes involved in ovulation rate and litter size in sheep. Resource: https://stud.epsilon.slu.se/6803/7/ jansson_t_140618.pdf, Date accessed: 28.03.2018.
- Karslı T, Şahin E, Argun Karslı B, Alkan S, Balcıoğlu MS. An investigation of mutations (FecXG, FecXI, FecXH, FecXB) on BMP-15 gene in some local sheep breeds raised in Turkey. Mediterr Agric Sci, 25(1): 29-33, 2012.
- Kasiriyan MM, Hafezeyan H, Sayahzadeh H, Jamshidi R, Asghari SR, Irajeyan GH, Buesagh H. Genetic polymorphism FecB and BMP15 genes and its association with litter size in Sangsari sheep breed of Iran. J Anim Vet Adv, 8(5): 1025-1031, 2009.
- Ligda Ch. Gabriilidis G, Papadopoulos Th, Georgoudis A. Estimation of genetic parameters for production traits of Chios sheep using a multitrait animal model. Livest Sci, 66: 217–221, 2000.
- Marjitha IS, Rajendran R, Sudhakar A, Raja A. Screening for Galway (FecXG ), Inverdale (FecXI ) and Belclare (FecXB) mutations in BMP15 gene in Indian Nilagiri sheep. Indian J Small Rumin, 21(2): 331-334, 2015.
- Mavrogenis AP, The fecundity of Chios sheep. In: Land, R.B., Robinson, D.W. (Eds.), Genetics of Reproduction in Sheep, Butterworths, London, pp 63–67, 1985.
- Mohammadi G. Determination of FecX, FecB and FecGH mutations in Iranian Arabic sheep. Bas.J.Vet.Res, 15(3): 435-445, 2016.
- Monteagudo LV, Ponz R, Tejedor MT, Lavina A, Sierra I. A 17 bp deletion in the Bone Morphogenetic Protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Ara-gonesa sheep breed. Anim Reprod Sci, 110: 139– 146, 2009.
- Mullen MP, Hanrahan JP, Howard DJ, Powell R. Investigation of prolific sheep from UK and Ireland for evidence on origin of the mutations in BMP15 (FecXG, FecXB) and GDF9 (FecGH) in Belclare and Cambridge Sheep. (Migaud M, ed.) PLoS ONE, 8(1):e53172, 2013, doi:10.1371/journal.pone.0053172. Notter DR. Genetic Aspects of Reproduction in Sheep. Dinçel et al. 2018 Repro Dom Anim, 43(2):122–128, 2008.
- Piper LR, Bindon BM. The BooroolaMerino and the performance of medium non-peppin crosses at Armidale. In: Piper LR, Bindon BM, Nethery RD (Eds.). The Booroola Merino, Proceedings of a Workshop, Armidale, CSIRO, pp. 9–19, 1982.
- Pramod KR, Sharma SK, Kumar R, Rajan A. Genetics of ovulation rate in farm animals. Vet World, 6(11), 833-838, 2013.
- Shi H, Bai J, Niu Z, Esha M, Jia B. Study on candidate gene for fecundity traits in Xingjiang Cele black sheep. Afr J Biotechnol, 9(49):8498-8505, 2010.
- Theodorikis A, Ragdos A, Roustemis D, Galanopoulos K, Abas Z, Sinapis E. Assessing technical efficiency of Chios sheep farms with data envelopment analysis. Small Ruminant Research, 107: 85– 9, 2012.
- Wan Somarny WMZ, Roziatul Erin AR, Suhaimi AHMS, Nurulhuda MO, Hifzan RM. A study of major prolificacy genes in Malin and Dorper sheep in Malaysia. J Trop Agric and Fd Sc, 41(2):265-272, 2013.
| APA | DİNÇEL D, ARDIÇLI S, ŞAMLI H, BALCI F (2018). Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. , 87 - 91. 10.30782/uluvfd.413857 |
| Chicago | DİNÇEL Deniz,ARDIÇLI Sena,ŞAMLI Hale,BALCI Faruk Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. (2018): 87 - 91. 10.30782/uluvfd.413857 |
| MLA | DİNÇEL Deniz,ARDIÇLI Sena,ŞAMLI Hale,BALCI Faruk Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. , 2018, ss.87 - 91. 10.30782/uluvfd.413857 |
| AMA | DİNÇEL D,ARDIÇLI S,ŞAMLI H,BALCI F Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. . 2018; 87 - 91. 10.30782/uluvfd.413857 |
| Vancouver | DİNÇEL D,ARDIÇLI S,ŞAMLI H,BALCI F Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. . 2018; 87 - 91. 10.30782/uluvfd.413857 |
| IEEE | DİNÇEL D,ARDIÇLI S,ŞAMLI H,BALCI F "Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep." , ss.87 - 91, 2018. 10.30782/uluvfd.413857 |
| ISNAD | DİNÇEL, Deniz vd. "Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep". (2018), 87-91. https://doi.org/10.30782/uluvfd.413857 |
| APA | DİNÇEL D, ARDIÇLI S, ŞAMLI H, BALCI F (2018). Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 37(2), 87 - 91. 10.30782/uluvfd.413857 |
| Chicago | DİNÇEL Deniz,ARDIÇLI Sena,ŞAMLI Hale,BALCI Faruk Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 37, no.2 (2018): 87 - 91. 10.30782/uluvfd.413857 |
| MLA | DİNÇEL Deniz,ARDIÇLI Sena,ŞAMLI Hale,BALCI Faruk Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol.37, no.2, 2018, ss.87 - 91. 10.30782/uluvfd.413857 |
| AMA | DİNÇEL D,ARDIÇLI S,ŞAMLI H,BALCI F Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2018; 37(2): 87 - 91. 10.30782/uluvfd.413857 |
| Vancouver | DİNÇEL D,ARDIÇLI S,ŞAMLI H,BALCI F Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2018; 37(2): 87 - 91. 10.30782/uluvfd.413857 |
| IEEE | DİNÇEL D,ARDIÇLI S,ŞAMLI H,BALCI F "Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep." Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 37, ss.87 - 91, 2018. 10.30782/uluvfd.413857 |
| ISNAD | DİNÇEL, Deniz vd. "Genotype frequency of $FecX^B$(Belclare) mutation of BMP15 genein Chios (Sakiz) sheep". Uludağ Üniversitesi Veteriner Fakültesi Dergisi 37/2 (2018), 87-91. https://doi.org/10.30782/uluvfd.413857 |
