TY  - JOUR
TI  - Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock
AB  - The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.
AU  - CETINKAYA, BURHAN
AU  - Timurkaan, Necati
AU  - Eröksüz, Hatice
AU  - ONGOR, Hasan
AU  - Coven, Fethiye
AU  - Carlı, Kamil Tayfun
AU  - KARABULUT, BURAK
DO  - 10.33988/auvfd.756970
PY  - 2021
JO  - Ankara Üniversitesi Veteriner Fakültesi Dergisi
VL  - 68
IS  - 2
SN  - 1300-0861
SP  - 167
EP  - 172
DB  - TRDizin
UR  - http://search/yayin/detay/407940
ER  -