The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model

KOLAÇ, Umut Kerem; Entok, Emre; ÜSTÜNER, MEHMET CENGIZ; Tekin Karacaer, Neslihan; AKYÜZ, FAHRETTIN; Alataş, İ. Özkan; tanrikut, cihan

doi:10.20515/otd.749410

The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model

Neslihan TEKİN, (Aksaray Üniversitesi, Fen Edebiyat Fakültesi, Biyoteknoloji ve Moleküler Biyoloji Bölümü, Aksaray, Türkiye)

Umut Kerem Kolac, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Aydın, Türkiye)

Cihan Tanrikut, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

M. Cengiz ÜSTÜNER, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

Emre Entok, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Nükleer Tıp Anabilim Dalı, Eskişehir, Türkiye)

FAHRETTİN AKYÜZ, (Eskişehir Osmangazi Üniversitesi, Tıbbi Biyokimya Anabilim Dalı Eskişehir, Türkiye)

İbrahim Özkan ALATAŞ (Eskişehir Osmangazi Üniversitesi, Tıbbi Biyokimya Anabilim Dalı, Eskişehir, Türkiye)

Osmangazi Tıp Dergisi

9 2

Yıl: 2021 Cilt: 43 Sayı: 2 Sayfa Aralığı: 94 - 105 Metin Dili: İngilizce DOI: 10.20515/otd.749410 İndeks Tarihi: 06-05-2021

The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model

Öz:

Lipopolysaccharide (LPS) is a main constituent of Gram-negative bacterial cell walls and is considered a leading cause of sepsis.S-allyl cysteine (SAC) is a water-soluble organosulfur component present in garlic which has a potent antioxidant and free radicalscavenger activity. The purpose of this study is to examine the antioxidant and anti-inflammatory potential of SAC on endotoxinLPS-induced sepsis. Female Wistar albino rats were divided into 6 groups. LPS (5 mg/kg) was applied to rats in sepsis and treatment groups intraperitoneally. After 24 hours from LPS injection 50 mg/kg and 100 mg/kg SAC was orally administrated totreatment groups. Lung and liver 18F-fluoro-deoxy-D-glucose (18F-FDG) uptake was measured by 18FDG-PET scan. Serumlevels of nuclear factor-kappa B (NF-κβ), tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase-9 (MMP-9), plasmalevels of interleukin-1β (IL-1β), IL-6 and tissue levels of oxidative stress markers catalase (CAT), superoxide dismutase (SOD),malondialdehyde (MDA) and nitric oxide (NO) were determined. As a result of the study, MDA and NO levels of sepsis groupwere significantly higher than treatment groups in lung tissue. SOD activities of sepsis group was determined to significantly lowerin the liver and lung tissues than the groups which were treated with SAC. Likewise, it was concluded that serum MMP-9, TNF-αand NF-κβ levels of sepsis group was significantly higher compared to levels of treatment groups. It was determined that SACadministration reduced 18F-FDG uptake in septic rats. In conclusion, SAC was observed to diminish effects of the acute toxicityand oxidative stress formed with LPS.

Anahtar Kelime:

Lipopolisakkarit ile İndüklenen Sıçan Sepsis Modelinde S-Allil Sisteinin İnflamatuar Kaskat Üzerine Etkileri

Öz:

Lipopolisakkarit (LPS), Gram-negatif bakteriyel hücre duvarlarının ana bileşenidir ve sepsisin önde gelen bir nedeni olarak kabul edilir. S-alil sistein (SAC), sarımsakta bulunan güçlü bir antioksidan ve serbest radikal temizleyici aktiviteye sahip suda çözünür bir organosülfür bileşenidir. Bu çalışmanın amacı, SAC’nin endotoksin LPS kaynaklı sepsis üzerindeki antioksidan ve antiinflamatuar potansiyelini incelemektir. Dişi Wistar albino sıçanları 6 gruba ayrıldı. LPS (5 mg/kg), sepsis ve tedavi gruplarındaki sıçanlara intraperitoenal olarak uygulandı. LPS enjeksiyonundan 24 saat sonra 50 mg/kg ve 100 mg/kg SAC oral olarak tedavi gruplarına uygulandı. Akciğer ve karaciğer 18F-floro-deoksi-D-glukoz (18F-FDG) alımı, 18FDG-PET taraması ile ölçüldü. Serum nükleer faktör-kappa B (NF-κβ), tümör nekroz faktörü-alfa (TNF-α), matriks metalloproteinaz-9 (MMP-9), interlökin-1β (IL-1β) seviyeleri, plazmada IL-6 seviyesi ve dokuda oksidatif stres markerleri katalaz (CAT), süperoksit dismutaz (SOD), malondialdehid (MDA) ve nitrik oksit (NO) seviyeleri belirlendi. Çalışma sonucunda, akciğer dokusunda MDA ve NO düzeyleri sepsis grubunda tedavi gruplarından anlamlı olarak yüksekti. Karaciğer ve akciğer dokularında SOD aktivitelerinin sepsis grubunda SAC ile tedavi edilen gruplara göre anlamlı derecede düşük olduğu belirlendi. Benzer şekilde sepsis grubu serum MMP-9, TNF-α ve NF-κβ düzeylerinin tedavi gruplarının düzeylerine göre anlamlı derecede yüksek olduğu sonucuna varıldı. Septik sıçanlarda SAC uygulamasının 18F-FDG alımını azalttığı belirlenmiştir. Sonuç olarak, SAC’nin LPS ile oluşan akut toksisite ve oksidatif stresin etkilerini azalttığı gözlenmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Tekin Karacaer N, KOLAÇ U, tanrikut c, ÜSTÜNER M, Entok E, AKYÜZ F, Alataş İ (2021). The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. , 94 - 105. 10.20515/otd.749410
Chicago	Tekin Karacaer Neslihan,KOLAÇ Umut Kerem,tanrikut cihan,ÜSTÜNER MEHMET CENGIZ,Entok Emre,AKYÜZ FAHRETTIN,Alataş İ. Özkan The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. (2021): 94 - 105. 10.20515/otd.749410
MLA	Tekin Karacaer Neslihan,KOLAÇ Umut Kerem,tanrikut cihan,ÜSTÜNER MEHMET CENGIZ,Entok Emre,AKYÜZ FAHRETTIN,Alataş İ. Özkan The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. , 2021, ss.94 - 105. 10.20515/otd.749410
AMA	Tekin Karacaer N,KOLAÇ U,tanrikut c,ÜSTÜNER M,Entok E,AKYÜZ F,Alataş İ The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. . 2021; 94 - 105. 10.20515/otd.749410
Vancouver	Tekin Karacaer N,KOLAÇ U,tanrikut c,ÜSTÜNER M,Entok E,AKYÜZ F,Alataş İ The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. . 2021; 94 - 105. 10.20515/otd.749410
IEEE	Tekin Karacaer N,KOLAÇ U,tanrikut c,ÜSTÜNER M,Entok E,AKYÜZ F,Alataş İ "The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model." , ss.94 - 105, 2021. 10.20515/otd.749410
ISNAD	Tekin Karacaer, Neslihan vd. "The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model". (2021), 94-105. https://doi.org/10.20515/otd.749410

APA	Tekin Karacaer N, KOLAÇ U, tanrikut c, ÜSTÜNER M, Entok E, AKYÜZ F, Alataş İ (2021). The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. Osmangazi Tıp Dergisi, 43(2), 94 - 105. 10.20515/otd.749410
Chicago	Tekin Karacaer Neslihan,KOLAÇ Umut Kerem,tanrikut cihan,ÜSTÜNER MEHMET CENGIZ,Entok Emre,AKYÜZ FAHRETTIN,Alataş İ. Özkan The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. Osmangazi Tıp Dergisi 43, no.2 (2021): 94 - 105. 10.20515/otd.749410
MLA	Tekin Karacaer Neslihan,KOLAÇ Umut Kerem,tanrikut cihan,ÜSTÜNER MEHMET CENGIZ,Entok Emre,AKYÜZ FAHRETTIN,Alataş İ. Özkan The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. Osmangazi Tıp Dergisi, vol.43, no.2, 2021, ss.94 - 105. 10.20515/otd.749410
AMA	Tekin Karacaer N,KOLAÇ U,tanrikut c,ÜSTÜNER M,Entok E,AKYÜZ F,Alataş İ The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. Osmangazi Tıp Dergisi. 2021; 43(2): 94 - 105. 10.20515/otd.749410
Vancouver	Tekin Karacaer N,KOLAÇ U,tanrikut c,ÜSTÜNER M,Entok E,AKYÜZ F,Alataş İ The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model. Osmangazi Tıp Dergisi. 2021; 43(2): 94 - 105. 10.20515/otd.749410
IEEE	Tekin Karacaer N,KOLAÇ U,tanrikut c,ÜSTÜNER M,Entok E,AKYÜZ F,Alataş İ "The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model." Osmangazi Tıp Dergisi, 43, ss.94 - 105, 2021. 10.20515/otd.749410
ISNAD	Tekin Karacaer, Neslihan vd. "The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model". Osmangazi Tıp Dergisi 43/2 (2021), 94-105. https://doi.org/10.20515/otd.749410