Objective: To evaluate the vascular structure of the choroid with choroidal vascular index (CVI) in amblyopic eyes. Ma-terial and Methods: Fifty nine patients followed up with the diagno-sis of amblyopia diagnosis and 30 healthy individuals of the similar age were included in the study. Refractive values, best corrected visual acu-ity (BCVA), causes of amblyopia, subfoveal choroidal thickness (SFCT), CVI values and relationship between these parameters were analyzed. ImageJ software was used on enhanced depth spectral do-main optical coherence tomography (EDI-OCT) images of the patients. With this software, the total choroidal area (TCA) was divided into lu-minal area (LA) and stromal area (SA). The ratio of LA to TCA was de-fined as CVI. Results: The study was performed on 89 eyes, including 24 eyes of 24 anisometropic amblyopia patients, 35 eyes of 35 strabis-mic amblyopia patients, and 30 eyes of 30 healthy individuals. The mean SFCT was 378.46±55.66 μm in the strabismic amblyopia group and 343.35±66.27 μm in the anisometropic amblyopia group. When the cur-rent values were compared with the control group, the increased SFCT in the strabismic amblyopia group was found to be statistically signifi-cant (p<0.001). In comparison of anisometropic amblyopia with the con-trol group and strabismic amblyopia group, no statistically significant difference was found (p>0.05). CVI was found as 63.27±1.08% in the control group, 63.78±1.75% in the strabismic amblyopia group, and 63.61±1.53% in the anisometropic amblyopia group. While the increase in CVI in strabismic amblyopia was statistically significant compared to the control group (p=0.046), the difference was not statistically signifi-cant in comparison of anisometropic amblyopia CVI values with the control and strabismic amblyopia group findings (p>0.05). Conclusion: SFCT and CVI values were found to be increased in eyes with strabis-mic amblyopia compared to anisometropic amblyopia and healthy eyes.