Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis

kurt, serdal; SALAR, Seçkin; Ozdal Salar, Merve

doi:10.33188/vetheder.509535

Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis

Serdal KURT, (Ankara Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Ankara, Türkiye)

Seçkin SALAR, (Ankara Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Ankara, Türkiye)

Merve ÖZDAL SALAR (Ankara Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Veteriner Hekimler Derneği Dergisi

6 0

Yıl: 2019 Cilt: 90 Sayı: 2 Sayfa Aralığı: 66 - 70 Metin Dili: İngilizce DOI: 10.33188/vetheder.509535 İndeks Tarihi: 08-05-2021

Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis

Öz:

The fertility success of dairy cows is greatly associated with their uterine health status in the postpartum period. However,the functioning of the uterus in cows is frequently put at risk due to contamination of the uterine lumen by pathogenic bacteria. The most common bacterial species causing uterine infections are Escherichia (E) coli, Trueperella (T) pyogenes, Streptococcus spp, Fusobacterium (F) necrophorum, Bacteroides and Prevotella. One of the most prevalent postpartum uterus diseases is defined as metritis. The objective of this study was to evaluate the bacterial insolation and antibacterial susceptibility resulting from vaginal discharges in dairy cows with metritis. The study was carried out on 15 multiparous (between 3 to 6 ages) Holstein dairy cows with metritis. Metritis was characterized by fever (>39.5°C), fetid watery red-brown vaginal discharge and systemic signs of diseases in the first 21 days postpartum. The vaginal swabs for microbiological analysis and the antimicrobial susceptibility test were taken under aseptic conditions from the cows. As a result, four different types of bacteria were isolated including E. coli (35.29%), T. pyogenes (29.41%), Streptococcus spp (17.65%) and Staphylococcus (S) aureus (17.65%). Then, 13 antimicrobial agents were used in the antibiogram test. It was found that the most effective antimicrobial agents were florfenicol, seftiofur, oxytetracycline, linkomycin and penicillin. Also, it was determined that some bacteria species have resistance against antimicrobial agents. In conclusion, it was concluded that the effects of antimicrobial agents and metritis agents changed from cow to cow. So, it was thought that uterine infections causing infertility can be restricted by the isolation of pathogen and the choice of appropriate antimicrobial agents.

Anahtar Kelime:

Metritisli sütçü ineklerde vaginal akıntıdan etken izolasyonu ve antibiyogram

Öz:

Sütçü ineklerde fertilite başarısı çoğunlukla postpartum dönemde uterus sağlığı ile ilişkilidir. Ancak, ineklerde uterusun normal işleyişi sıklıkla patojen bakterilerin uterus lumenini kontamine etmesiyle riske atılır. Uterus enfeksiyonlarınaneden olan en yaygın bakteri türleri Escherichia (E) coli, Trueperella (T) pyogenes, Streptococcus spp, Fusobacterium (F) necrophorum, Bacteroides ve Prevotella dır. En sık karşılaşılan uterus hastalıklarından biri metritis’tir. Bu çalışmanınamacı, metritisli ineklerin vaginal akıntılarından bakteri izolasyonu ve bu bakterilere karşı etkili antibiyotikleri tespitetmekti. Sunulan çalışmada 3 ile 6 yaş arasında 15 adet metritisli multipar Holstein sütçü inek kullanıldı. Metritis,doğumdan sonraki ilk 21 gün içinde ateş (> 39.5 ° C), sulu kırmızı-kahverengi ve kokulu uterus akıntısı ve sistemikhastalık belirtileri ile karakterize edildi. Metritisli ineklerden mikrobiyolojik analiz ve antimikrobiyal hassasiyet testi içinstreil şartlarda vajinal svab alındı. Analizlerde, E. coli (%35.29), T. pyogenes (%29.41), Streptococcus spp (%17.65) veStaphylococcus (S) aureus (%17.65) dahil olmak üzere dört farklı bakteri türü izole edildi. Antiogram testinde 13antimikrobiyal ajan kullanıldı ve en etkili antimikrobiyal ajanların florfenikol, seftiofur, oksitetrasiklin, linkomisin vepenisilin olduğu tespit edildi. Ayrıca bazı bakteri türlerinin antimikrobiyal ajanlara karşı direnç gösterdiği belirlendi.Sonuç olarak, metritis etkenlerinin ve antimikrobiyal ajanların etkilerinin inekten ineğe değiştiği görüldü. Bu nedenle,infertiliteye neden olan uterus enfeksiyonlarının etken izolasyonu ve doğru antibiyotiklerin seçimi ile kısıtlanabileceğidüşünüldü.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	kurt s, SALAR S, Ozdal Salar M (2019). Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. , 66 - 70. 10.33188/vetheder.509535
Chicago	kurt serdal,SALAR Seçkin,Ozdal Salar Merve Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. (2019): 66 - 70. 10.33188/vetheder.509535
MLA	kurt serdal,SALAR Seçkin,Ozdal Salar Merve Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. , 2019, ss.66 - 70. 10.33188/vetheder.509535
AMA	kurt s,SALAR S,Ozdal Salar M Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. . 2019; 66 - 70. 10.33188/vetheder.509535
Vancouver	kurt s,SALAR S,Ozdal Salar M Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. . 2019; 66 - 70. 10.33188/vetheder.509535
IEEE	kurt s,SALAR S,Ozdal Salar M "Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis." , ss.66 - 70, 2019. 10.33188/vetheder.509535
ISNAD	kurt, serdal vd. "Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis". (2019), 66-70. https://doi.org/10.33188/vetheder.509535

APA	kurt s, SALAR S, Ozdal Salar M (2019). Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. Veteriner Hekimler Derneği Dergisi, 90(2), 66 - 70. 10.33188/vetheder.509535
Chicago	kurt serdal,SALAR Seçkin,Ozdal Salar Merve Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. Veteriner Hekimler Derneği Dergisi 90, no.2 (2019): 66 - 70. 10.33188/vetheder.509535
MLA	kurt serdal,SALAR Seçkin,Ozdal Salar Merve Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. Veteriner Hekimler Derneği Dergisi, vol.90, no.2, 2019, ss.66 - 70. 10.33188/vetheder.509535
AMA	kurt s,SALAR S,Ozdal Salar M Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. Veteriner Hekimler Derneği Dergisi. 2019; 90(2): 66 - 70. 10.33188/vetheder.509535
Vancouver	kurt s,SALAR S,Ozdal Salar M Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis. Veteriner Hekimler Derneği Dergisi. 2019; 90(2): 66 - 70. 10.33188/vetheder.509535
IEEE	kurt s,SALAR S,Ozdal Salar M "Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis." Veteriner Hekimler Derneği Dergisi, 90, ss.66 - 70, 2019. 10.33188/vetheder.509535
ISNAD	kurt, serdal vd. "Antibiogram and pathogen isolation from vaginal discharge in dairy cows withmetritis". Veteriner Hekimler Derneği Dergisi 90/2 (2019), 66-70. https://doi.org/10.33188/vetheder.509535