TY  - JOUR
TI  - Four Temperate Bacteriophages from Methicillin-resistant Staphylococcus aureus Show Broad Bactericidal and Biofilm Removal Activities
AB  - The emergence of multi-drug resistance among many bacteria including zoonotic pathogens in the food chain poses a growing public health threatto humans, animals, and the environment worldwide. The inefficiency of current antibiotics to control these pathogens necessitated the developmentof alternative approaches, such as phage therapy, for the prevention and treatment of human and animal infections, food safety, and wastewatertreatment. In this study, four temperate bacteriophages, designated as Trsa205, Trsa207, Trsa220, and Trsa222 were isolated by mitomycin C inductionfrom methicillin-resistant Staphylococcus aureus (MRSA) strains. The phages were characterized based on their electron microscope morphology, burstsize, host range, and biofilm removal potential. Based on their morphology, all four phages with isometric heads and long non-contractile tails belongto Siphoviridae family. The one-step growth curves of phages revealed that Trsa205 and Trsa207 have latent periods of about 20 min that results in aburst size of 30 and 45 virions/host cell, respectively, while Trsa220 and Trsa222 showed 25 min of latent period and produced 20 virus particles/cell.The agar-spot assay was used for phage host range determination, and biofilm removal activities were measured spectrophotometrically after crystalviolet staining. It was found that at least two-thirds of 56 S. aureus strains (66%) could be lysed by phages when used in combination, and 20-38% byone of the phages. The four phages in combination were able to remove the S. aureus biofilms by 65%. Our results indicated that the newly identifiedbacteriophages have the potential to be used in phage therapy against multi-drug resistant S. aureus including MRSA and removal of biofilms.
AU  - Durukan, İnci
AU  - KHORSHIDTALAB, MONA
AU  - Kilic, Ali Osman
AU  - Abdurahman, Mujib Abdulkadir
AU  - TOSUN, Ilknur
DO  - 10.9775/kvfd.2020.24680
PY  - 2021
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 27
IS  - 1
SN  - 1300-6045
SP  - 29
EP  - 36
DB  - TRDizin
UR  - http://search/yayin/detay/412132
ER  -