Aim: In this study, we aimed to evaluate the levels of brown adipose tissue markers uncoupling protein-1 (UCP-1), irisin, bonemorphogenetic protein-7 (BMP-7) and PR-Domain Zinc Finger Protein-16 (PRDM-16) in Cushing’s disease (CH) with hypercortisolism.Methods: The study was conducted prospectively with 48 CH patients and 40 healthy volunteers between 2018 and 2019. Two ccof peripheral blood taken from the participants was centrifuged and stored at -80 oC degrees. Cushing’s syndrome was excluded byperforming 1 mg dexamethasone suppression test in the control group. Blood samples were analyzed by Enzyme-linked ImmunosorbentAssay method.Results: The patient group included 11 males (22.9%), 37 female (77.1%); 9 male (22.5%) and 31 females (77.5%) in the controlgroup. Body mass index was 31.29±5.76 kg/m2 in the patient group and 33.42±3.11 kg/m2 in the control group. PRDM-16, Irisin, BMP-7, UCP-1 levels were not significantly different between the two groups. While there was a positive correlation between serum cortisoland irisin, a negative correlation was observed between urinary free cortisol and UCP-1.Conclusion: These data suggest that long-term exposure to high doses of glucocorticoids in CH patients causes loss of adipose tissuefunctionality and development of resistance in vivo.