Effect of black grape extract on MMP-9 gene expression in breast cancer cells

Ahu SOYACAK, (İstanbul Aydın Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İstanbul, Türkiye)
Gülşah (İstanbul Aydın Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İstanbul, Türkiye)
Biological Diversity and Conservation
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Yıl: 2020 Cilt: 3 Sayı: 3 Sayfa Aralığı: 194 - 199 Metin Dili: İngilizce DOI: 10.46309/biodicon.2020. 824105 İndeks Tarihi: 28-05-2021

Effect of black grape extract on MMP-9 gene expression in breast cancer cells

Öz:
Metastasis is among the most important factors in the decrease in breast cancer survival and treatment failure.It is of great importance to elucidate the molecular pathways of breast cancer metastasis. Recent research shows thatpolyphenolic compounds in plants have anti-invasive and anti-metastatic activities. Molecular mechanism of blackgrape extract including abundant polyphenols on breast cancer and metastasis are not yet fully known. In our study, itwas aimed to determine the effect of black grape extract applied to human breast cancer cell line on cell viability andadhesion and on MMP-9 gene expression, which is thought to have a role in metastasis. In the study, cell viability bytrypan blue method, cell adhesion with 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate (XTT) method and matrix metalloproteinases 9 (MMP-9) gene expression by qRT-PCR weredetermined in MCF-7 breast cancer cell lines. In our study, it was found that black grape extract decreased MMP-9 geneexpression, cell viability and adhesion and this decrease was not statistically significant (p> 0.05). The study resultssupport the hypothesis that natural anti-cancer agents such as polyphenols can be prevent cancer to occur and spread byacting on molecules such as MMP-9. However, further studies are needed to elucidate the molecular mechanisms ofpolyphenols
Anahtar Kelime:

Siyah üzüm ekstresinin meme kanseri hücrelerinde MMP-9 gen ekspresyonu üzerine etkisi

Öz:
Meme kanseri sağ kalım oranındaki düşüş ve tedavisindeki başarısızlığın en önemli etkenleri arasında metastaz görülmektedir. Meme kanseri metastazının moleküler yolaklarının aydınlatılması büyük bir öneme sahiptir. Son yıllarda yapılan araştırmalar, bitkilerde bulunan polifenolik bileşiklerin anti-invaziv ve anti-metastatik yeteneklere sahip olduğunu göstermektedir. Bol miktarda polifenol içeren siyah üzüm ekstresinin meme kanseri ve metastazı üzerindeki etkisi ve moleküler mekanizması henüz tam olarak bilinmemektedir. Çalışmamızda, insan meme kanseri hücre dizisine uygulanan siyah üzüm ekstresinin hücre canlılığı ve adezyonuna etkisi ile metastazda rolü olabileceği düşünülen MMP9 gen ekspresyonu üzerine etkisinin belirlenmesi amaçlanmıştır. MCF-7 meme kanser hücre dizilerinde tripan blue yöntemi ile hücre canlılığı, sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate (XTT) yöntemi ile hücre adezyonu ve qRT-PCR ile matriks metalloproteinaz 9 (MMP-9) gen ekspresyonu belirlenmiştir. Çalışmamızda, siyah üzüm ekstresinin MMP-9 gen ekspresyonunu, hücre canlılığını ve adezyonunu azalttığı, bu azalmanın istatistiksel olarak anlamlı olmadığı bulunmuştur (p>0.05). Sonuç olarak polifenoller gibi doğal kanser önleyici ajanların MMP-9 gibi kanserin oluşmasını ve yayılmasını sağlayan moleküllere etki ederek önleyebileceği fikri desteklenmiştir. Bununla birlikte polifenollerin etkilediği moleküler mekanizmaların aydınlatılabilmesi için ileri çalışmalara ihtiyaç vardır.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA SOYOCAK A, Koc G (2020). Effect of black grape extract on MMP-9 gene expression in breast cancer cells. , 194 - 199. 10.46309/biodicon.2020. 824105
Chicago SOYOCAK Ahu,Koc Gulsah Effect of black grape extract on MMP-9 gene expression in breast cancer cells. (2020): 194 - 199. 10.46309/biodicon.2020. 824105
MLA SOYOCAK Ahu,Koc Gulsah Effect of black grape extract on MMP-9 gene expression in breast cancer cells. , 2020, ss.194 - 199. 10.46309/biodicon.2020. 824105
AMA SOYOCAK A,Koc G Effect of black grape extract on MMP-9 gene expression in breast cancer cells. . 2020; 194 - 199. 10.46309/biodicon.2020. 824105
Vancouver SOYOCAK A,Koc G Effect of black grape extract on MMP-9 gene expression in breast cancer cells. . 2020; 194 - 199. 10.46309/biodicon.2020. 824105
IEEE SOYOCAK A,Koc G "Effect of black grape extract on MMP-9 gene expression in breast cancer cells." , ss.194 - 199, 2020. 10.46309/biodicon.2020. 824105
ISNAD SOYOCAK, Ahu - Koc, Gulsah. "Effect of black grape extract on MMP-9 gene expression in breast cancer cells". (2020), 194-199. https://doi.org/10.46309/biodicon.2020. 824105
APA SOYOCAK A, Koc G (2020). Effect of black grape extract on MMP-9 gene expression in breast cancer cells. Biological Diversity and Conservation, 3(3), 194 - 199. 10.46309/biodicon.2020. 824105
Chicago SOYOCAK Ahu,Koc Gulsah Effect of black grape extract on MMP-9 gene expression in breast cancer cells. Biological Diversity and Conservation 3, no.3 (2020): 194 - 199. 10.46309/biodicon.2020. 824105
MLA SOYOCAK Ahu,Koc Gulsah Effect of black grape extract on MMP-9 gene expression in breast cancer cells. Biological Diversity and Conservation, vol.3, no.3, 2020, ss.194 - 199. 10.46309/biodicon.2020. 824105
AMA SOYOCAK A,Koc G Effect of black grape extract on MMP-9 gene expression in breast cancer cells. Biological Diversity and Conservation. 2020; 3(3): 194 - 199. 10.46309/biodicon.2020. 824105
Vancouver SOYOCAK A,Koc G Effect of black grape extract on MMP-9 gene expression in breast cancer cells. Biological Diversity and Conservation. 2020; 3(3): 194 - 199. 10.46309/biodicon.2020. 824105
IEEE SOYOCAK A,Koc G "Effect of black grape extract on MMP-9 gene expression in breast cancer cells." Biological Diversity and Conservation, 3, ss.194 - 199, 2020. 10.46309/biodicon.2020. 824105
ISNAD SOYOCAK, Ahu - Koc, Gulsah. "Effect of black grape extract on MMP-9 gene expression in breast cancer cells". Biological Diversity and Conservation 3/3 (2020), 194-199. https://doi.org/10.46309/biodicon.2020. 824105
Sayfa Oluşturulma Tarihi
03-05-2024 9:49

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