Yıl: 2020 Cilt: 4 Sayı: 3 Sayfa Aralığı: 230 - 236 Metin Dili: İngilizce DOI: 10.30621/jbachs.2020.1057 İndeks Tarihi: 02-06-2021

Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy

Öz:
Purpose: Radiocontrast-induced nephropathy is an important clinical problem in high-risk patients. The mechanism of cytotoxic effect of radiocontrast media on kidneys is still not fully clarified. Rac1/β-PAK pathway has been shown to play a role in other nephropathies such as diabetic nephropathy. In this study, it was aimed to determine whether Rac1/β-PAK signalling pathway has any role in the development of contrast-induced nephropathy (CIN). Methods: Adult male Balb/C mice were used. A single dose of iohexol was given intraperitoneally. Thirty-two mice were divided into 5 groups including control (Group1, n=4), pretreatment (Group 2, n=7), low dose (2 g iodine/kg) iohexol (Group 3, n=7), medium dose (2.5 g iodine/kg) iohexol (Group 4, n=7) and high dose (3 g iodine/kg) iohexol (Group 5, n=7). The animals were sacrificed 24 hours after iohexol administration. Nephropathy were evaluated by histological and biochemical analysis methods. Expressions of Rac1 and β-PAK were evaluated by immunohistochemical method. Apoptosis was assessed by TUNEL method. Results: It was found that severity of nephropathy, apoptosis and β-PAK expressions significantly increased as iohexol dosage increased. Although Rac1 expression was higher in the iohexol group in comparison to the control and pretreatment groups, its increase did not show a dose-dependent manner. There was no significant difference among the groups in terms of serum creatinine. Serum cystatin C were increased in all groups compared to the control group, but significant increase was observed only in the pretreatment group. Conclusions: Our findings suggest that the Rac1/β-PAK signal transduction pathway may have a role in the contrast-induced nephropathy model.
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APA Manisalıgil A, Cilaker Micili S, kizildag s, Çelik A, Bağrıyanık A, Gumustekin M (2020). Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. , 230 - 236. 10.30621/jbachs.2020.1057
Chicago Manisalıgil Aysun,Cilaker Micili Serap,kizildag servet,Çelik Aslı,Bağrıyanık Alper,Gumustekin Mukaddes Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. (2020): 230 - 236. 10.30621/jbachs.2020.1057
MLA Manisalıgil Aysun,Cilaker Micili Serap,kizildag servet,Çelik Aslı,Bağrıyanık Alper,Gumustekin Mukaddes Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. , 2020, ss.230 - 236. 10.30621/jbachs.2020.1057
AMA Manisalıgil A,Cilaker Micili S,kizildag s,Çelik A,Bağrıyanık A,Gumustekin M Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. . 2020; 230 - 236. 10.30621/jbachs.2020.1057
Vancouver Manisalıgil A,Cilaker Micili S,kizildag s,Çelik A,Bağrıyanık A,Gumustekin M Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. . 2020; 230 - 236. 10.30621/jbachs.2020.1057
IEEE Manisalıgil A,Cilaker Micili S,kizildag s,Çelik A,Bağrıyanık A,Gumustekin M "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy." , ss.230 - 236, 2020. 10.30621/jbachs.2020.1057
ISNAD Manisalıgil, Aysun vd. "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy". (2020), 230-236. https://doi.org/10.30621/jbachs.2020.1057
APA Manisalıgil A, Cilaker Micili S, kizildag s, Çelik A, Bağrıyanık A, Gumustekin M (2020). Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences, 4(3), 230 - 236. 10.30621/jbachs.2020.1057
Chicago Manisalıgil Aysun,Cilaker Micili Serap,kizildag servet,Çelik Aslı,Bağrıyanık Alper,Gumustekin Mukaddes Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences 4, no.3 (2020): 230 - 236. 10.30621/jbachs.2020.1057
MLA Manisalıgil Aysun,Cilaker Micili Serap,kizildag servet,Çelik Aslı,Bağrıyanık Alper,Gumustekin Mukaddes Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences, vol.4, no.3, 2020, ss.230 - 236. 10.30621/jbachs.2020.1057
AMA Manisalıgil A,Cilaker Micili S,kizildag s,Çelik A,Bağrıyanık A,Gumustekin M Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences. 2020; 4(3): 230 - 236. 10.30621/jbachs.2020.1057
Vancouver Manisalıgil A,Cilaker Micili S,kizildag s,Çelik A,Bağrıyanık A,Gumustekin M Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy. Journal of Basic and Clinical Health Sciences. 2020; 4(3): 230 - 236. 10.30621/jbachs.2020.1057
IEEE Manisalıgil A,Cilaker Micili S,kizildag s,Çelik A,Bağrıyanık A,Gumustekin M "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy." Journal of Basic and Clinical Health Sciences, 4, ss.230 - 236, 2020. 10.30621/jbachs.2020.1057
ISNAD Manisalıgil, Aysun vd. "Assessment of Rac1 and β-PAK Expressions in a Mouse Model for Contrast-Induced Nephropathy". Journal of Basic and Clinical Health Sciences 4/3 (2020), 230-236. https://doi.org/10.30621/jbachs.2020.1057