A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients

Kasali, Kamber; akaras, nurhan; HALICI, Mesut; Ozgül  Abuc, Ozlem; Demirci, Tuba

doi:10.14744/ijmb.2020.83007

A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients

Nurhan AKARAS, (Aksaray Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Aksaray, Türkiye)

Tuba DEMİRCİ, (Atatürk Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Özlem Özgül ABUÇ, (Erzincan Binali Yıldırım Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzincan, Türkiye)

MESUT BÜNYAMİ HALICI, (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)

KAMBER KAŞALİ (Atatürk Üniversitesi, Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Erzurum, Türkiye)

International Journal of Medical Biochemistry

2 0

Yıl: 2021 Cilt: 4 Sayı: 1 Sayfa Aralığı: 42 - 49 Metin Dili: İngilizce DOI: 10.14744/ijmb.2020.83007 İndeks Tarihi: 05-06-2021

A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients

Öz:

Objectives: The aim of this study was to investigate the effect of chemical changes in the follicular fluid and histological changes in the cumulus cells of the oocyte microenvironment on the number of oocytes in infertile patients.Methods: A total of 50 female patients aged 18-35 who presented at the Atatürk University Research Hospital Infertility Clinic and for infertility treatment were included. The patients were divided into 3 groups: Patients with fewerthan 5 oocytes were classified as Group 1, patients with 5-20 oocytes comprised Group 2, and Group 3 was made upof patients with >20 oocytes. During the oocyte collection process, follicular fluid was aspirated from the follicles andthe cumulus cells were collected. The follicular fluid was stored at -80°C for use in biochemical analysis of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), superoxide dismutase (SOD), glutathione (GSH).Immunohistochemical staining was performed to examine caspase-3 and mechanistic target of rapamycin (mTOR)immunoreactivity at the stereological level.Results: The MDA level and total oxidant capacity (TOC) in the follicular fluid were higher in Group 1 patients than inthe other 2 groups, while the SOD was lower (p<0.05). In Group 2 patients, the MDA level and TOS were higher thanthose of Group 3, while the SOD level was lower (p<0.05). The total antioxidant capacity (TAC) and GSH levels did notvary significantly according to the number of oocytes (p<0.05). Immunohistochemical staining showed that mTOR andcaspase-3 immunoreactivity were more intense in Group 1 than in the other groups.Conclusion: The increase in mTOR expression may activate the caspase-3 pathway, which could lead to oxidativestress. The mTOR pathway may affect the oocyte count

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	akaras n, Demirci T, Ozgül Abuc O, HALICI M, Kasali K (2021). A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. , 42 - 49. 10.14744/ijmb.2020.83007
Chicago	akaras nurhan,Demirci Tuba,Ozgül Abuc Ozlem,HALICI Mesut,Kasali Kamber A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. (2021): 42 - 49. 10.14744/ijmb.2020.83007
MLA	akaras nurhan,Demirci Tuba,Ozgül Abuc Ozlem,HALICI Mesut,Kasali Kamber A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. , 2021, ss.42 - 49. 10.14744/ijmb.2020.83007
AMA	akaras n,Demirci T,Ozgül Abuc O,HALICI M,Kasali K A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. . 2021; 42 - 49. 10.14744/ijmb.2020.83007
Vancouver	akaras n,Demirci T,Ozgül Abuc O,HALICI M,Kasali K A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. . 2021; 42 - 49. 10.14744/ijmb.2020.83007
IEEE	akaras n,Demirci T,Ozgül Abuc O,HALICI M,Kasali K "A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients." , ss.42 - 49, 2021. 10.14744/ijmb.2020.83007
ISNAD	akaras, nurhan vd. "A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients". (2021), 42-49. https://doi.org/10.14744/ijmb.2020.83007

APA	akaras n, Demirci T, Ozgül Abuc O, HALICI M, Kasali K (2021). A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. International Journal of Medical Biochemistry, 4(1), 42 - 49. 10.14744/ijmb.2020.83007
Chicago	akaras nurhan,Demirci Tuba,Ozgül Abuc Ozlem,HALICI Mesut,Kasali Kamber A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. International Journal of Medical Biochemistry 4, no.1 (2021): 42 - 49. 10.14744/ijmb.2020.83007
MLA	akaras nurhan,Demirci Tuba,Ozgül Abuc Ozlem,HALICI Mesut,Kasali Kamber A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. International Journal of Medical Biochemistry, vol.4, no.1, 2021, ss.42 - 49. 10.14744/ijmb.2020.83007
AMA	akaras n,Demirci T,Ozgül Abuc O,HALICI M,Kasali K A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. International Journal of Medical Biochemistry. 2021; 4(1): 42 - 49. 10.14744/ijmb.2020.83007
Vancouver	akaras n,Demirci T,Ozgül Abuc O,HALICI M,Kasali K A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. International Journal of Medical Biochemistry. 2021; 4(1): 42 - 49. 10.14744/ijmb.2020.83007
IEEE	akaras n,Demirci T,Ozgül Abuc O,HALICI M,Kasali K "A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients." International Journal of Medical Biochemistry, 4, ss.42 - 49, 2021. 10.14744/ijmb.2020.83007
ISNAD	akaras, nurhan vd. "A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients". International Journal of Medical Biochemistry 4/1 (2021), 42-49. https://doi.org/10.14744/ijmb.2020.83007