The Anatolian Jird Meriones tristrami is a widespread rodent species in Anatolian steps. In the study, to determine the relationships between the subpopulations of species and to clarify their distributions, 233 adults samples representing Turkey under the 12 groups, according to their recorded geographical localities, were compared biometrically. For this purpose, measurements of the four external morphological and twenty-seven cranial characters of the groups were analyzed statistically. Despite the high similarity of their biometric characters due to their belonging to the same species, subpopulations were separated from each other with small differences. In the cluster tree (UPGMA), Manisa and Kastamonu subpopulations were noticeably similar with Karaman and Ankara subpopulations. Kilis and Şanlıurfa subpopulations were linked to the Western and Central Anatolian groups, and these two subpopulations were observed to be close to the groups that include Malatya and Kayseri samples. Iğdır subpopulation was among the Central and Western Anatolia and Southeastern Anatolia groups. The existence of these biometric relationships in the groups supports the hypothesis that the spread of this species to the west of Anatolia should have been by following a route from the north rather than the south of Central Anatolia. In addition, it is predicted that biometric differentiation between subspecies is formed by the effect of geographical features of Anatolia.