TY  - JOUR
TI  - The Effect of Doxycycline on Glomerulosclerosis in 5/6 Renal Ablation Nephropathy
AB  - Objectives: The beneficial effect of matrix metalloproteinases (MMP) inhibitors in renal diseases has been reported. Their effect in segmentalsclerosis is unknown. The aim of this study is to investigate the effect of a MMP inhibitor, doxycycline, onglomeruloscleresis (GS) in renal ablation nephropathy and to evaluate the MMP-2, MMP-9, tissue inhibitors of matrix metalloproteinases (TIMP)-1,TIMP-2, and collagen type IV expressions.Materials and Methods: Fourteen of the 32 female Wistar albinos were 5/6 nephrectomised. Doxycyline was given to half of each group (40 mg/kg/day total 28 days). After sacrification, the GS, MMP-2 and MMP-9 expressions, and TIMP-2 expressions were analyzed histopathologically. Proand active MMP-2 and -9 were analyzed by gelatin zymography. TIMP-1 and TIMP-2 were measured with the enzyme-linked immunoassay.Results: Doxycycline administration to the 5/6 nephrectomy group improved GS but did not inhibit glomerular MMP-9 or cortical pro- and active-MMP-2 and pro-MMP9 but increased TIMP-1 and TIMP-2 expression in all groups in cortical tissue. Type IV collagen was decreased in the groupswhere GS were increased. MMP-9 expression and GS were increased in all groups receiving doxycycline.Conclusion: We have demonstrated improved GS in renal ablation model by doxycycline administration but also doxycycline has an unexpectedadverse effect. The effect of doxycycline on the expression of MMP-2 and -9 cannot explain the improvement in GS but increased cortical TIPM-1and -2 may be an important contributing factor for the inhibition of MMPs. Other types of MMPs and TIMPs may be important. Accumulation ofother types of collagen may be prominent in GS of ablation nephropathy.
AU  - BULUT ARIKAN, Funda
AU  - Cavdar, Zahide
AU  - Sarioglu, Sulen
AU  - Yılmaz, Osman
AU  - Kahraman, Devrim
AU  - Can, İlkay Nur
AU  - Kolatan, Hatice Efsun
DO  - 10.4274/atfm.galenos.2020.60362
PY  - 2020
JO  - Ankara Üniversitesi Tıp Fakültesi Mecmuası
VL  - 73
IS  - 3
SN  - 0365-8104
SP  - 290
EP  - 299
DB  - TRDizin
UR  - http://search/yayin/detay/421708
ER  -