TY  - JOUR
TI  - Utilization of Cheese Whey for Production of Azurin by Pseudomonas aeruginosa
AB  - Azurin which has attracted much attention as potential anticancer agent in recent years is abacterial secondary metabolite. This copper-containing redox protein secreted by Pseudomonasaeruginosa has capability of preferentially entering into many human cancer cells and inducingapoptosis. In this study, whey which is the considerable by-product of the casein or cheesemanufacture was used as azurin production medium by P. aeruginosa. Also, effects of copper(II) sulphate (CuSO4) and potassium nitrate (KNO3) on the azurin production were determined.At the end of the studies, optimum azurin expression level was reached during the incubationof 18 hours. The best CuSO4 concentration was 2.5 mg/L while the best KNO3 concentrationwas 45 mg/L according to Western blot analysis. This process can be used to obtain high levelsof azurin using P. aeruginosa in whey medium. Also, using whey for azurin production canreduce many processing industrial whey waste management problems.
AU  - Unver, Yagmur
DO  - 10.16984/saufenbilder.853961
PY  - 2021
JO  - Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
VL  - 25
IS  - 2
SN  - 1301-4048
SP  - 601
EP  - 609
DB  - TRDizin
UR  - http://search/yayin/detay/421839
ER  -