ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT
Yıl: 2020 Cilt: 22 Sayı: 3 Sayfa Aralığı: 450 - 460 Metin Dili: İngilizce DOI: 10.24938/kutfd.789547 İndeks Tarihi: 14-06-2021
ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT
Öz: Objective: The spread of antibiotic resistance genes among bacteriacauses serious problems in the treatment of infectious diseases.Recently, it has been shown that these resistance genes are also found inintegrons. This study investigated the class 1, 2 integrons and theantibiotic-resistant genes in the infection agent Acinetobacter baumanniiproduced in respiratory tract samples.Material and Methods: The study involved 86 A. baumannii strainsisolated from lower respiratory tract samples collected between March2014 and March 2015 in the Intensive Care Units. Identification andantibiograms of the isolates were made using conventional methods andVitek 2 Compact systems. The presence of integrons in strains wasinvestigated by polymerase chain reaction method using specific primerpairs for class 1and class 2 integrase regions. All samples in whichintegron amplification was carried out were subjected to DNA sequenceanalysis both by cloning and as a PCR product. The resistance geneswere screened by polymerase chain reaction.Results: Isolates were obtained from specimens including trachealaspirates (33.7%), bronchoalveolar lavage (29.1%), sputum (22.1%),bronchial washing fluid/bronchial brushing (4.7%), transbronchialbiopsy (4.7%), pleural fluid (3.4%) and lung aspirates/abscess (2.3%).While resistance to imipenem, meropenem, ampicillin-sulbactam,ceftazidime, and piperacillin-tazobactam was determined in all isolates,resistance rates to ciprofloxacin, levofloxacin, gentamicin, amikacin andtigecycline were 97.7%, 93.0%, 60.5%, 53.5%and 9.3%, respectively.Colistin was the only antibiotic to which all strains were susceptible.The positivity rates of integrase I and II genes were 45.4%and 9.3%,respectively. Class 1 and 2 integron positivity rates in all isolates were31.4%and 8.1%, respectively. blaTEM, blaSHV, blaCTX-M1, blaCTXM2,blaOXA23, blaOXA40, blaOXA58 were 70.9%, 2.3%, 4.7%, 7.0%,95.3%, 5.8%and 3.5%, respectively.Conclusion: Increasing association of multi drug resistant strains andintegron resistant genes in A. baumannii infections will lead to thefailure of antibiotic treatment strategies.
Anahtar Kelime: Yoğun Bakım Ünitesinden Alınan Alt Solunum Yolu Örneklerinde Üretilen Acinetobacter baumannii İzolatlarında Antibiyotik Direnci ve İntegron Gen Kasetleri
Öz: Amaç: Antibiyotik direnç genlerinin bakteriler arasındaki yayılımı, enfeksiyon hastalıklarının tedavisinde ciddi sorunlara yol açmaktadır. Son zamanlarda bu direnç genlerin integronlarda da bulunduğu gösterilmiştir. Bu çalışmada alt solunum yollarından alınan kültürlerde üretilen infeksiyon etkeni olan Acinetobacter baumannii suşlarında sınıf 1, sınıf 2 integron varlığı ve antibiyotik direnç genleri araştırıldı. Gereç ve Yöntemler: Çalışmaya, Mart 2014- Mart 2015 tarihleri arasında alt solunum yolu örneklerinden izole edilen toplam 86 A. baumannii suşu dahil edildi. İzolatların tanımlanması ve antibiyogramları konvansiyonel metodlar ve Vitek 2 Compact sistemleriyle yapıldı. Suşlarda integron varlığı, sınıf 1 (intI1) ve sınıf 2 (intI2) integraz bölgeleri için özgül primer çiftleri kullanılarak PCR yöntemiyle araştırıldı. İntegron amplifikasyonunun gerçekleştirildiği tüm örnekler hem klonlanarak hem de PCR ürünü olarak DNA dizi analizine tabi tutuldu. Direnç genleri polimeraz zincir reaksiyonu ile tarandı. Bulgular: İzolatlar trakeal aspiratlar (%33.7), bronkoalveoler lavaj (%29,1), balgam (%22.1), bronşiyal yıkama sıvısı / bronşiyal fırçalama (%4.7), transbronşiyal biyopsi (%4.7), plevral sıvı (%3.4) ve akciğer aspirasyonları / apse (%2.3) örneklerinden elde edildi. Tüm izolatlarda imipenem, meropenem, seftazidim, piperasillintazobaktam, ampisilin-sulbaktama karşı direnç saptanırken, siprofloksasin, levofloksasin, gentamisin, amikasin ve tigesikline karşı direnç sırasıyla %97.7, %93.0, %60.5, %53.5 ve %9.3 olarak tespit edildi. Kolistin, tüm suşlara karşı tek etkin antibiyotikti. Integraz I ve II gen pozitiflik oranı sırasıyla %45.4 ve %9.3 iken, sınıf 1 ve 2 integron pozitiflik oranı tüm izolatlarda sırasıyla %31.4 ve %8.1 olarak bulundu. blaTEM, blaSHV, blaCTX-M1, blaCTX-M2, blaOXA23, blaOXA40, blaOXA58 sırasıyla %70.9, %2.3, %4.7, %7.0, %95.3, %5.8 ve %3.5 bulundu. Sonuç: A. baumannii infeksiyonlarında çoklu ilaç direnci olan suşların ve integron direnç genlerinin birlikte artışı, antibiyotik tedavi stratejilerinin başarısızlığına yol açacaktır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | kostakoglu u, erturk a, YILDIZ I, ÖZYURT S, COPUR CİCEK A, Karataş M, Sönmez E (2020). ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. , 450 - 460. 10.24938/kutfd.789547 |
| Chicago | kostakoglu ugur,erturk ayse,YILDIZ ILKNUR ESEN,ÖZYURT Songül,COPUR CİCEK AYSEGUL,Karataş Mevlüt,Sönmez Emine ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. (2020): 450 - 460. 10.24938/kutfd.789547 |
| MLA | kostakoglu ugur,erturk ayse,YILDIZ ILKNUR ESEN,ÖZYURT Songül,COPUR CİCEK AYSEGUL,Karataş Mevlüt,Sönmez Emine ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. , 2020, ss.450 - 460. 10.24938/kutfd.789547 |
| AMA | kostakoglu u,erturk a,YILDIZ I,ÖZYURT S,COPUR CİCEK A,Karataş M,Sönmez E ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. . 2020; 450 - 460. 10.24938/kutfd.789547 |
| Vancouver | kostakoglu u,erturk a,YILDIZ I,ÖZYURT S,COPUR CİCEK A,Karataş M,Sönmez E ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. . 2020; 450 - 460. 10.24938/kutfd.789547 |
| IEEE | kostakoglu u,erturk a,YILDIZ I,ÖZYURT S,COPUR CİCEK A,Karataş M,Sönmez E "ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT." , ss.450 - 460, 2020. 10.24938/kutfd.789547 |
| ISNAD | kostakoglu, ugur vd. "ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT". (2020), 450-460. https://doi.org/10.24938/kutfd.789547 |
| APA | kostakoglu u, erturk a, YILDIZ I, ÖZYURT S, COPUR CİCEK A, Karataş M, Sönmez E (2020). ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 22(3), 450 - 460. 10.24938/kutfd.789547 |
| Chicago | kostakoglu ugur,erturk ayse,YILDIZ ILKNUR ESEN,ÖZYURT Songül,COPUR CİCEK AYSEGUL,Karataş Mevlüt,Sönmez Emine ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 22, no.3 (2020): 450 - 460. 10.24938/kutfd.789547 |
| MLA | kostakoglu ugur,erturk ayse,YILDIZ ILKNUR ESEN,ÖZYURT Songül,COPUR CİCEK AYSEGUL,Karataş Mevlüt,Sönmez Emine ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, vol.22, no.3, 2020, ss.450 - 460. 10.24938/kutfd.789547 |
| AMA | kostakoglu u,erturk a,YILDIZ I,ÖZYURT S,COPUR CİCEK A,Karataş M,Sönmez E ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi. 2020; 22(3): 450 - 460. 10.24938/kutfd.789547 |
| Vancouver | kostakoglu u,erturk a,YILDIZ I,ÖZYURT S,COPUR CİCEK A,Karataş M,Sönmez E ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT. Kırıkkale Üniversitesi Tıp Fakültesi Dergisi. 2020; 22(3): 450 - 460. 10.24938/kutfd.789547 |
| IEEE | kostakoglu u,erturk a,YILDIZ I,ÖZYURT S,COPUR CİCEK A,Karataş M,Sönmez E "ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT." Kırıkkale Üniversitesi Tıp Fakültesi Dergisi, 22, ss.450 - 460, 2020. 10.24938/kutfd.789547 |
| ISNAD | kostakoglu, ugur vd. "ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT". Kırıkkale Üniversitesi Tıp Fakültesi Dergisi 22/3 (2020), 450-460. https://doi.org/10.24938/kutfd.789547 |
