TY  - JOUR
TI  - ANTIBIOTIC RESISTANCE AND INTEGRON GENE CASSETTES IN ACINETOBACTER BAUMANNII ISOLATES PRODUCED IN LOWER RESPIRATORY TRACT SAMPLES TAKEN FROM THE INTENSIVE CARE UNIT
AB  - Objective: The spread of antibiotic resistance genes among bacteriacauses serious problems in the treatment of infectious diseases.Recently, it has been shown that these resistance genes are also found inintegrons. This study investigated the class 1, 2 integrons and theantibiotic-resistant genes in the infection agent Acinetobacter baumanniiproduced in respiratory tract samples.Material and Methods: The study involved 86 A. baumannii strainsisolated from lower respiratory tract samples collected between March2014 and March 2015 in the Intensive Care Units. Identification andantibiograms of the isolates were made using conventional methods andVitek 2 Compact systems. The presence of integrons in strains wasinvestigated by polymerase chain reaction method using specific primerpairs for class 1and class 2 integrase regions. All samples in whichintegron amplification was carried out were subjected to DNA sequenceanalysis both by cloning and as a PCR product. The resistance geneswere screened by polymerase chain reaction.Results: Isolates were obtained from specimens including trachealaspirates (33.7%), bronchoalveolar lavage (29.1%), sputum (22.1%),bronchial washing fluid/bronchial brushing (4.7%), transbronchialbiopsy (4.7%), pleural fluid (3.4%) and lung aspirates/abscess (2.3%).While resistance to imipenem, meropenem, ampicillin-sulbactam,ceftazidime, and piperacillin-tazobactam was determined in all isolates,resistance rates to ciprofloxacin, levofloxacin, gentamicin, amikacin andtigecycline were 97.7%, 93.0%, 60.5%, 53.5%and 9.3%, respectively.Colistin was the only antibiotic to which all strains were susceptible.The positivity rates of integrase I and II genes were 45.4%and 9.3%,respectively. Class 1 and 2 integron positivity rates in all isolates were31.4%and 8.1%, respectively. blaTEM, blaSHV, blaCTX-M1, blaCTXM2,blaOXA23, blaOXA40, blaOXA58 were 70.9%, 2.3%, 4.7%, 7.0%,95.3%, 5.8%and 3.5%, respectively.Conclusion: Increasing association of multi drug resistant strains andintegron resistant genes in A. baumannii infections will lead to thefailure of antibiotic treatment strategies.
AU  - erturk, ayse
AU  - Karataş, Mevlüt
AU  - Sönmez, Emine
AU  - YILDIZ, ILKNUR ESEN
AU  - kostakoglu, ugur
AU  - COPUR CİCEK, AYSEGUL
AU  - ÖZYURT, Songül
DO  - 10.24938/kutfd.789547
PY  - 2020
JO  - Kırıkkale Üniversitesi Tıp Fakültesi Dergisi
VL  - 22
IS  - 3
SN  - 2148-9645
SP  - 450
EP  - 460
DB  - TRDizin
UR  - http://search/yayin/detay/421858
ER  -