TY  - JOUR
TI  - Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)
AB  - Objective: Members of the Apiaceae family have many biological andpharmacological effects like anticancer, antidiabetic, antioxidant,anti-inflammatory, anti-hypertensive, anticoagulant, antimicrobial,and anticholinesterase. Also, some of them are consumed asvegetables, pickles, and spices. Carbonic anhydrase has a remarkablerole in diseases like osteoporosis, glaucoma, edema, cancer, epilepsy,and obesity. The inhibition effects of methanol extracts and subextracts (n-hexane, dichloromethane, ethyl acetate, n-butanol, andaqueous residue) of Leiotulus dasyanthus and Ferulago pauciradiataroots on carbonic anhydrase I and II isoenzymes were investigatedin this research.Methods: Leiotulus dasyanthus and Ferulago pauciradiata werecollected in 2017 from Erzurum and in 2013 from Nevşehir(Turkey), respectively. The roots of L. dasyanthus and F. pauciradiatawere macerated with methanol, dispersed with methanol: distilledwater (1:9), and fractionated with n-hexane, dichloromethane, ethylacetate, and n-butanol, in turn. The hCA I and II isoenzymes were isolated from erythrocytes of fresh human blood via Sepharose-4BL-Tyrosine-sulfanilamide affinity chromatography. The analysis ofhCA I and II isoenzymes was actualized by the Verpoorte method.Results: The hCA I and II were influentially inhibited by thedichloromethane and ethyl acetate sub-extracts of F. pauciradiataroot with IC50 values of 1.694 μg/mL towards hCA I and 1.677 μg/mL towards hCA II, respectively. Whereas dichloromethane subextract and methanol extract of L. dasyanthus root inhibited hCA Iand II isoenzymes with IC50 values of 1.828 μg/mL towards hCA Iand 1.852 μg/mL towards hCA II, respectively.Conclusion: The hCA I and II were influentially inhibited bythe dichloromethane and ethyl acetate sub-extracts of Ferulagopauciradiata root.
AU  - Gulcin, ilhami
AU  - bingöl, zeynebe
AU  - DELİMUSTAFAOĞLU BOSTANLIK, Fatmagül
AU  - Kılıç, Ceyda Sibel
AU  - DUMAN, HAYRI
AU  - KARAKAYA, Songül
DO  - 10.14235/bas.galenos.2020.4127
PY  - 2021
JO  - Bezmiâlem Science
VL  - 9
IS  - 2
SN  - 2148-2373
SP  - 153
EP  - 157
DB  - TRDizin
UR  - http://search/yayin/detay/422290
ER  -