Yıl: 2021 Cilt: 9 Sayı: 2 Sayfa Aralığı: 185 - 189 Metin Dili: İngilizce DOI: 10.14235/bas.galenos.2020.4159 İndeks Tarihi: 14-06-2021

Impact of UCP2 -866G/A Variant on Smoking Risk

Öz:
Objective: Mitochondria are multifunctional and dynamicorganelles found in cells. Nicotine is a natural alkaloid found inthe tobacco plant and has been well studied as a component ofcigarette smoke. It has also been reported to affect mitochondrialfunction both in vitro and in vivo. Uncoupling protein 2 (UCP2)reduces generation of ROS by mitochondria. Our purpose in thisstudy was to investigate whether the -866G/A variant of the UCP2gene is associated with smoking status.Methods: A total of 238 individuals consisting of 138 smokers and100 healthy controls were examined. The UCP2-866G/A variantwas genotyped by polymerase chain reaction-restriction fragmentlength polymorphism method.Results: The proportion of individuals carrying the three possiblegenotype was significantly different between the smoker and healthycontrol groups. The UCP2-866G/A variant GG genotype wasassociated significantly with an increased risk of smoking (p=0.001)while AA genotype was associated significantly with a decreased riskof smoking (p=0.001). The UCP2-866G/A variant G allele wasfound to be increased in the smoker group compared to the healthycontrols (p=0.001).Conclusion: Our data suggest that the UCP2-866 G/A variant GGgenotype and G allele might reflect the risk of smoking status in aTurkish population
Anahtar Kelime:

UCP2 -866G/A Varyantının Sigara İçme Durumuna Etkisi

Öz:
Amaç: Mitokondriler hücrelerde bulunan çok fonksiyonlu ve dinamik organellerdir. Nikotin tütün bitkisinde bulunan doğal bir alkaloiddir ve sigara içme durumunun iyi çalışılmış bir bileşenidir. Nikotinin in vitro ve in vivo olarak mitokondriyal fonksiyonu etkilediği rapor edilmiştir. Uncoupling protein 2 (UCP2) mitokondri ile ROS üretimini azaltır. Bu çalışmadaki amacımız UCP2-866G/A varyantının sigara içme durumu ile ilişkili olup olmadığını araştırmaktır. Yöntemler: Yüz otuz sekiz sigara içen ve 100 sağlıklı kontrolden oluşan toplam 238 kişi değerlendirildi. UCP2-866G/A varyantı polimeraz zincir reaksiyonu-sınırlayıcı enzim parça uzunluk çeşitliliği metodu ile genotiplendi. Bulgular: Üç olası genotipi taşıyan kişileri oranı sigara içenler ve sağlıklı kontrol grupları arasında belirgin şekilde farklıydı. UCP2- 866G/A varyantı AA genotipi belirgin şekilde azalmış sigara içme riski ile ilişkiyken (p=0,001), GG genotipi belirgin şekilde artmış sigara içme durumu ile ilişkiliydi (p=0,001). UCP2-866G/A varyant G alelinin sigara içen grupta sağlıklı kontrollere göre artmış olduğu bulundu (p=0,001). Sonuç: Verilerimiz UCP2-866 G/A varyant GG genotip ve G alelin Türk popülasyonundaki sigara içme riskini yansıtabileceğini desteklemektedir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Dimijian GG. Evolving together: the biology of symbiosis, part 2. Proc (Bayl Univ Med Cent) 2000;13:381-90.
  • 2. Sadakierska-Chudy A, Frankowska M, Filip M. Mitoepigenetics and drug addiction. Pharmacol Ther 2014;144:226-33.
  • 3. Malińska D, Więckowski MR, Michalska B, Drabik K, Prill M, Patalas-Krawczyk P, et al. Mitochondria as a possible target for nicotine action. J Bioenerg Biomembr 2019;51:259-76.
  • 4. Jin Y, Chen D, Hu Y, Guo S, Sun H, Lu A, et al. Association between monoamine oxidase gene polymorphisms and smoking behaviour in Chinese males. Int J Neuropsychopharmacol 2006;9:557-64.
  • 5. Cormier A, Morin C, Zini R, Tillement JP, Lagrue G. Nicotine protects rat brain mitochondria against experimental injuries. Neuropharmacology 2003;44:642-52.
  • 6. Van Jaarsveld H, Kuyl JM, Alberts DW. Antioxidant vitamin supplementation of smoke-exposed rats partially protects against myocardial ischaemic/reperfusion injury. Free Radic Res Commun 1992;17:263-9.
  • 7. van Jaarsveld H, Kuyl JM, Alberts DW. Exposure of rats to low concentration of cigarette smoke increases myocardial sensitivity to ischaemia/reperfusion. Basic Res Cardiol 1992;87:393-9.
  • 8. Garlid KD, Jaburek M, Jezek P. Mechanism of uncoupling protein action. Biochem Soc Trans 2001;29:803-6.
  • 9. Souza BM, Assmann TS, Kliemann LM, Gross JL, Canani LH, Crispim D. The role of uncoupling protein 2 (UCP2) on the development of type 2 diabetes mellitus and its chronic complications. Arq Bras Endocrinol Metabol 2011;55:239-48.
  • 10. Richard D, Clavel S, Huang Q, Sanchis D, Ricquier D. Uncoupling protein 2 in the brain: distribution and function. Biochem Soc Trans 2001;29:812-7.
  • 11. Heatherton TF, Kozlowski LT, Frecker RC, Fagerström KO. The Fagerström Test for Nicotine Dependence: a revision of the Fagerström Tolerance Questionnaire. Br J Addict 1991;86:1119-27.
  • 12. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215.
  • 13. Srivastava N, Prakash J, Lakhan R, Agarwal CG, Pant DC, Mittal B. A common polymorphism in the promoter of UCP2 is associated with obesity and hyperinsulenemia in northern Indians. Mol Cell Biochem 2010;337:293-8.
  • 14. Echtay KS. Mitochondrial uncoupling proteins--what is their physiological role? Free Radic Biol Med 2007;43:1351-71.
  • 15. Le Houezec J. Role of nicotine pharmacokinetics in nicotine addiction and nicotine replacement therapy: a review. Int J Tuberc Lung Dis 2003;7:811-9.
  • 16. Vani G, Anbarasi K, Shyamaladevi CS. Bacoside A: Role in Cigarette Smoking Induced Changes in Brain. Evid Based Complement Alternat Med 2015;2015:286137.
  • 17. Zhang XY, Tan YL, Zhou DF, Haile CN, Wu GY, Cao LY, et al. Nicotine dependence, symptoms and oxidative stress in male patients with schizophrenia. Neuropsychopharmacology 2007;32:2020-4.
  • 18. Anbarasi K, Vani G, Devi CS. Protective effect of bacoside A on cigarette smoking-induced brain mitochondrial dysfunction in rats. J Environ Pathol Toxicol Oncol 2005;24:225-34.
  • 19. Moylan S, Jacka FN, Pasco JA, Berk M. How cigarette smoking may increase the risk of anxiety symptoms and anxiety disorders: a critical review of biological pathways. Brain Behav 2013;3:302-26.
  • 20. Normoyle KP, Kim M, Farahvar A, Llano D, Jackson K, Wang H. The emerging neuroprotective role of mitochondrial uncoupling protein-2 in traumatic brain injury. Transl Neurosci 2015;6:179-86.
  • 21. Cornwell BR, Arkin N, Overstreet C, Carver FW, Grillon C. Distinct contributions of human hippocampal theta to spatial cognition and anxiety. Hippocampus 2012;22:1848-59.
  • 22. Gimsa U, Kanitz E, Otten W, Aheng C, Tuchscherer M, Ricquier D, et al. Alterations in anxiety-like behavior following knockout of the uncoupling protein 2 (ucp2) gene in mice. Life Sci 2011;89:677-84.
  • 23. Dietrich MO, Andrews ZB, Horvath TL. Exercise-induced synaptogenesis in the hippocampus is dependent on UCP2-regulated mitochondrial adaptation. J Neurosci 2008;28:10766-71.
  • 24. Andrews ZB, Rivera A, Elsworth JD, Roth RH, Agnati L, Gago B, et al. Uncoupling protein-2 promotes nigrostriatal dopamine neuronal function. Eur J Neurosci 2006;24:32-6.
  • 25. Li C, Sun H, Arrick DM, Mayhan WG. Chronic nicotine exposure exacerbates transient focal cerebral ischemia-induced brain injury. J Appl Physiol (1985) 2016;120:328-33.
  • 26. Krempler F, Esterbauer H, Weitgasser R, Ebenbichler C, Patsch JR, Miller K, et al. A functional polymorphism in the promoter of UCP2 enhances obesity risk but reduces type 2 diabetes risk in obese middle-aged humans. Diabetes 2002;51:3331-5.
  • 27. Wang H, Chu WS, Lu T, Hasstedt SJ, Kern PA, Elbein SC. Uncoupling protein-2 polymorphisms in type 2 diabetes, obesity, and insulin secretion. Am J Physiol Endocrinol Metab 2004;286:1-7.
APA Nursal A, UYSAL M, PEHLİVAN M, Sever Ü, pehlivan s (2021). Impact of UCP2 -866G/A Variant on Smoking Risk. , 185 - 189. 10.14235/bas.galenos.2020.4159
Chicago Nursal Ayse Feyda,UYSAL MEHMET ATILLA,PEHLİVAN MUSTAFA,Sever Ülgen,pehlivan sacide Impact of UCP2 -866G/A Variant on Smoking Risk. (2021): 185 - 189. 10.14235/bas.galenos.2020.4159
MLA Nursal Ayse Feyda,UYSAL MEHMET ATILLA,PEHLİVAN MUSTAFA,Sever Ülgen,pehlivan sacide Impact of UCP2 -866G/A Variant on Smoking Risk. , 2021, ss.185 - 189. 10.14235/bas.galenos.2020.4159
AMA Nursal A,UYSAL M,PEHLİVAN M,Sever Ü,pehlivan s Impact of UCP2 -866G/A Variant on Smoking Risk. . 2021; 185 - 189. 10.14235/bas.galenos.2020.4159
Vancouver Nursal A,UYSAL M,PEHLİVAN M,Sever Ü,pehlivan s Impact of UCP2 -866G/A Variant on Smoking Risk. . 2021; 185 - 189. 10.14235/bas.galenos.2020.4159
IEEE Nursal A,UYSAL M,PEHLİVAN M,Sever Ü,pehlivan s "Impact of UCP2 -866G/A Variant on Smoking Risk." , ss.185 - 189, 2021. 10.14235/bas.galenos.2020.4159
ISNAD Nursal, Ayse Feyda vd. "Impact of UCP2 -866G/A Variant on Smoking Risk". (2021), 185-189. https://doi.org/10.14235/bas.galenos.2020.4159
APA Nursal A, UYSAL M, PEHLİVAN M, Sever Ü, pehlivan s (2021). Impact of UCP2 -866G/A Variant on Smoking Risk. Bezmiâlem Science, 9(2), 185 - 189. 10.14235/bas.galenos.2020.4159
Chicago Nursal Ayse Feyda,UYSAL MEHMET ATILLA,PEHLİVAN MUSTAFA,Sever Ülgen,pehlivan sacide Impact of UCP2 -866G/A Variant on Smoking Risk. Bezmiâlem Science 9, no.2 (2021): 185 - 189. 10.14235/bas.galenos.2020.4159
MLA Nursal Ayse Feyda,UYSAL MEHMET ATILLA,PEHLİVAN MUSTAFA,Sever Ülgen,pehlivan sacide Impact of UCP2 -866G/A Variant on Smoking Risk. Bezmiâlem Science, vol.9, no.2, 2021, ss.185 - 189. 10.14235/bas.galenos.2020.4159
AMA Nursal A,UYSAL M,PEHLİVAN M,Sever Ü,pehlivan s Impact of UCP2 -866G/A Variant on Smoking Risk. Bezmiâlem Science. 2021; 9(2): 185 - 189. 10.14235/bas.galenos.2020.4159
Vancouver Nursal A,UYSAL M,PEHLİVAN M,Sever Ü,pehlivan s Impact of UCP2 -866G/A Variant on Smoking Risk. Bezmiâlem Science. 2021; 9(2): 185 - 189. 10.14235/bas.galenos.2020.4159
IEEE Nursal A,UYSAL M,PEHLİVAN M,Sever Ü,pehlivan s "Impact of UCP2 -866G/A Variant on Smoking Risk." Bezmiâlem Science, 9, ss.185 - 189, 2021. 10.14235/bas.galenos.2020.4159
ISNAD Nursal, Ayse Feyda vd. "Impact of UCP2 -866G/A Variant on Smoking Risk". Bezmiâlem Science 9/2 (2021), 185-189. https://doi.org/10.14235/bas.galenos.2020.4159