Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?
Yıl: 2021 Cilt: 14 Sayı: 2 Sayfa Aralığı: 354 - 360 Metin Dili: İngilizce DOI: 10.31362/patd.789163 İndeks Tarihi: 29-07-2022
Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?
Öz: Purpose: The aim of this experimental rat study is to investigate whether vancomycin powder directly andtopically applied on the sciatic nerve causes perineural fibrosis and chronic inflammation development.Materials and Methods: Thirty young adult male Wistar-Albino rats were included in the study and dividedinto 3 groups of 10 rats based on the dose to be applied after intraperitoneal anesthesia. After the sciaticnerve exploration, 10 rats (group 1) were administered a single dose of vancomycin powder, 10 rats (group 2)were administered a double dose of vancomycin, and control group was created with 10 animals which wereadministered no medication (group 3). After 3 weeks, rats were sacrificed, tissue samples were taken aroundsciatic nerve and sent for histopathological examination.Results: Grade 1 perineural fibrosis was detected in 1 animal (10%) in group 1. Grade 2 perineural fibrosis wasdetected in 1 animal (10%) and grade 1 perineural fibrosis was detected in 1 of 10 animals (10%) in group 2. InGroup 3, grade 1 perineural fibrosis was detected in 1 experimental animal (10%). Chronic inflammation wasnot detected in any experimental animal. No significant difference was found between the 3 groups in terms ofperineural fibrosis (p=0.753).Conclusion: In this experimental animal study, we found that fibrosis development did not differ significantlyamong the groups. We believe that more objective results can be obtained as a result of experimental studieswith larger participation and more detailed histopathological examinations.
Anahtar Kelime: Periferik sinir üzerinde topikal uygulanan vancomisin toz perinöral fibrozise neden olur mu?
Öz: Amaç: Bu deneysel sıçan çalışmasının amacı direk olarak siyatik sinir üzerine topikal uygulanan vancomisin tozun perinöral fibrozis ve kronik inflamasyon gelişimine neden olup olmadığını araştırmaktır. Material and methods: Otuz adet erkek Wistar-Albino cins sıçan çalışmaya dahil edilerek intraperitoneal anestezi sonrası uygulanacak doz miktarına göre 10’arlı 3 gruba ayrıldı. Siyatik sinir ekplorasyonu ardından tek doz vankomisin toz verilen 10 sıçan (grup 1), çift doz vankomisin uygulanan 10 sıçan (grup 2) , 10 hayvana ise ilaç verilmeyerek kontrol grubu (grup 3) oluşturuldu. 3 hafta sonra ise sıçanlar sakrifiye edilerek siyatik sinir etrafındaki doku örnekleri alındı ve histopatolojik incelemeye gönderildi. Bulgular: Grup 1’de 1 (%10) hayvanda evre 1 perinöral fibrosis tespit edildi. Diğer 9 (%90) deney hayvanında epidural fibrozis görülmedi. Grup 2’deki 10 deney hayvanından 1 tanesinde (%10) evre 2, 1 tanesinde ise (%10) evre 1 perinöral fibrozis saptandı. Sekiz hayvanda (%80) ise perinöral fibrozis bulgusu saptanmadı. Grup 3 de ise 1 (%10) deney hayvanında evre 1 perinöral fibrozis saptandı. Kronik inflamasyon ise hiçbir deney hayvanında tespit edilmedi. İstatistiksel incelemeler sonucunda 3 grup arasında perinöral fibrozis açısından anlamlı farklılık saptanmadı (p=0,753). Sonuç: Bu deneysel hayvan çalışmasında vankomisin toz uygulanan hastalarda fibrozis gelişiminin gruplar arasında anlamlı farklılık göstermediğini saptadık. Daha büyük katılımlı ve histopatolojik incelemelerin daha detaylı yapılacağı deneysel çalışmalar sonucunda daha objektif sonuçlara varılacağı kanaatindeyiz.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Fei Q, Li J, Lin J, et al. Risk factors for surgical site infection after spinal surgery: a meta-analysis. World Neurosurg 2016;95:507-515. http://doi.org/10.1016/j. wneu.2015.05.059
- 2. Meredith DS, Kepler CK, Huang RC, Brause BD, Boachie Adjei O. Postoperative infections of the lumbar spine: presentation and management. Int Orthop 2012;36:439-444. http://doi.org/10.1007/s00264-011- 1427-z
- 3. Pull Ter Gunne AF, Cohen DB. Incidence, prevalence, and analysis of risk factors for surgical site infection following adult spinal surgery. Spine 2009;34:1422- 1428. http://doi.org/10.1097/BRS.0b013e3181a03013
- 4. Bruniera FR, Ferreira FM, Saviolli LRM, et al. The use of vancomycin with its therapeutic and adverse effects: a review. Eur Rev Med Pharmacol Sci 2015;19:694- 700.
- 5. Clippinger B, Ayzenberg M, Narvaez M, et al. Effect of intrasite vancomycin powder on the development of epidural fibrosis. Orthop Rev 2018;10:7777. http://doi. org/10.4081/or.2018.7777
- 6. Ghobrial GM, Cadotte DW, Jr Williams K, Fehlings MG, Harrop JS. Complications from the use of intrawound vancomycin in lumbar spinal surgery: a systematic review. Neurosurg Focus 2015;39:11. http://doi. org/10.3171/2015.7.FOCUS15258
- 7. Texakalidis P, Lu VM, Yolcu Y, et al. Impact of powdered vancomycin on preventing surgical site infections in neurosurgery: a systematic review and meta-analysis. Neurosurgery 2019;84:569-580. http://doi.org/10.1093/ neuros/nyy288
- 8. Nahm FS, Lee PB, Choe GY, Lim YJ, Kim YC. Therapeutic effect of epidural hyaluronic acid in a rat model of foraminal stenosis. J Pain Res 2017;10:241- 248. http://doi.org/10.2147/JPR.S122861
- 9. Chilukuri DM, Shah JC. Local delivery of vancomycin for the prophylaxis of prosthetic device-related infections. Pharm Res 2005;22:563-572. http://doi. org/10.1007/s11095-005-2497-7
- 10. Godil SS, Parker SL, O’Neill KR, Devin CJ, McGirt MJ. Comparative effectiveness and cost-benefit analysis of local application of vancomycin powder in posterior spinal fusion for spine trauma: clinical article. J Neurosurg Spine 2013;19:331-335. http://doi. org/10.3171/2013.6.SPINE121105
- 11. Sweet FA, Roh M, Sliva C. Intrawound application of vancomycin for prophylaxis in instrumented thoracolumbar fusions: efficacy, drug levels, and patient outcomes. Spine 2011;36:2084-2088. http://doi. org/10.1097/BRS.0b013e3181ff2cb1
- 12. Strom RG, Pacione D, Kalhorn SP, Frempong Boadu AK. Lumbar laminectomy and fusion with routine local application of vancomycin powder: decreased infection rate in instrumented and non-instrumented cases. Clin Neurol Neurosurg 2013;115:1766-1769. http://doi. org/10.1016/j.clineuro.2013.04.005
- 13. Salafia CM, Weigl C, Silberman L. The prevalence and distribution of acute placental inflammation in uncomplicated term pregnancies. Obstet Gynecol 1989;73:383-389.
- 14. Schroeder JE, Girardi FP, Sandhu H, Weinstein J, Cammisa FP, Sama A. The use of local vancomycin powder in degenerative spine surgery. Eur Spine J 2016;25:1029-1033. http://doi.org/10.1007/s00586- 015-4119-3
- 15. Heller A, McIff TE, Lai SM, Burton DC. Intrawound vancomycin powder decreases staphylococcal surgical site infections after posterior instrumented spinal arthrodesis. J Spinal Disord Tech 2015;28:584-589. http://doi.org/10.1097/BSD.0000000000000045
- 16. Theologis AA, Demirkiran G, Callahan M, Pekmezci M, Ames C, Deviren V. Local intrawound vancomycin powder decreases the risk of surgical site infections in complex adult deformity reconstruction: a cost analysis. Spine 2014;39:1875-1880. http://doi.org/10.1097/ BRS.0000000000000533
- 17. Edin ML, Miclau T, Lester GE, Lindsey RW, Dahners LE. Effect of cefazolin and vancomycin on osteoblasts in vitro. Clin Orthop Relat Res 1996:245-251.
- 18. Emohare O, Ledonio CG, Hill BW, Davis RA, Polly DW, Kang MM. Cost savings analysis of intrawound vancomycin powder in posterior spinal surgery. Spine J 2014;14:2710-2715. http://doi.org/10.1016/j. spinee.2014.03.011
| APA | Olguner S, celiktas m, Erdogan K, Daglioglu Y, iştemen i, Arslan A, acik v, ÖKTEN A, GEZERCAN Y (2021). Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. , 354 - 360. 10.31362/patd.789163 |
| Chicago | Olguner Semih kıvanç,celiktas mustafa,Erdogan Kivilcim,Daglioglu Yusuf Kenan,iştemen ismail,Arslan Ali,acik vedat,ÖKTEN ALİ İHSAN,GEZERCAN YURDAL Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. (2021): 354 - 360. 10.31362/patd.789163 |
| MLA | Olguner Semih kıvanç,celiktas mustafa,Erdogan Kivilcim,Daglioglu Yusuf Kenan,iştemen ismail,Arslan Ali,acik vedat,ÖKTEN ALİ İHSAN,GEZERCAN YURDAL Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. , 2021, ss.354 - 360. 10.31362/patd.789163 |
| AMA | Olguner S,celiktas m,Erdogan K,Daglioglu Y,iştemen i,Arslan A,acik v,ÖKTEN A,GEZERCAN Y Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. . 2021; 354 - 360. 10.31362/patd.789163 |
| Vancouver | Olguner S,celiktas m,Erdogan K,Daglioglu Y,iştemen i,Arslan A,acik v,ÖKTEN A,GEZERCAN Y Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. . 2021; 354 - 360. 10.31362/patd.789163 |
| IEEE | Olguner S,celiktas m,Erdogan K,Daglioglu Y,iştemen i,Arslan A,acik v,ÖKTEN A,GEZERCAN Y "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?." , ss.354 - 360, 2021. 10.31362/patd.789163 |
| ISNAD | Olguner, Semih kıvanç vd. "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?". (2021), 354-360. https://doi.org/10.31362/patd.789163 |
| APA | Olguner S, celiktas m, Erdogan K, Daglioglu Y, iştemen i, Arslan A, acik v, ÖKTEN A, GEZERCAN Y (2021). Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pamukkale Tıp Dergisi, 14(2), 354 - 360. 10.31362/patd.789163 |
| Chicago | Olguner Semih kıvanç,celiktas mustafa,Erdogan Kivilcim,Daglioglu Yusuf Kenan,iştemen ismail,Arslan Ali,acik vedat,ÖKTEN ALİ İHSAN,GEZERCAN YURDAL Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pamukkale Tıp Dergisi 14, no.2 (2021): 354 - 360. 10.31362/patd.789163 |
| MLA | Olguner Semih kıvanç,celiktas mustafa,Erdogan Kivilcim,Daglioglu Yusuf Kenan,iştemen ismail,Arslan Ali,acik vedat,ÖKTEN ALİ İHSAN,GEZERCAN YURDAL Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pamukkale Tıp Dergisi, vol.14, no.2, 2021, ss.354 - 360. 10.31362/patd.789163 |
| AMA | Olguner S,celiktas m,Erdogan K,Daglioglu Y,iştemen i,Arslan A,acik v,ÖKTEN A,GEZERCAN Y Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pamukkale Tıp Dergisi. 2021; 14(2): 354 - 360. 10.31362/patd.789163 |
| Vancouver | Olguner S,celiktas m,Erdogan K,Daglioglu Y,iştemen i,Arslan A,acik v,ÖKTEN A,GEZERCAN Y Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?. Pamukkale Tıp Dergisi. 2021; 14(2): 354 - 360. 10.31362/patd.789163 |
| IEEE | Olguner S,celiktas m,Erdogan K,Daglioglu Y,iştemen i,Arslan A,acik v,ÖKTEN A,GEZERCAN Y "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?." Pamukkale Tıp Dergisi, 14, ss.354 - 360, 2021. 10.31362/patd.789163 |
| ISNAD | Olguner, Semih kıvanç vd. "Does vancomycin powder topically apply to the peripheral nerve cause perineural fibrosis?". Pamukkale Tıp Dergisi 14/2 (2021), 354-360. https://doi.org/10.31362/patd.789163 |
