TY  - JOUR
TI  - Alcoholic extract of Tarantula cubensis (Theranekron®) induce autophagy on gastric cancer cells
AB  - Aim: To evaluate the effects of theranekron in respect of autophagy on gastric cancer that is the fifth leadingcancer type worldwide.Metods: In the present study, metastatic AGS and non-metastatic MKN-45 human gastric cell lines were usedtogether with HEK-293 non-cancer cells as controls. Cytotoxic effect of theranekron besides appropriatetreatment time was investigated through cell proliferation by using Cell Proliferation assay Kit (MTT) usingdifferent concentrations of the drug. The autophagic effect of the drug was determined using the LC3-GFPtranslocation assay and western blot analysis. All experiments were performed also using the ethanol sinceTarantula cubensis spider was processed and diluted in 60% alcohol to generate as a drug.Results: MTT assay results demonstrated that the half maximal inhibitory concentration of theranekron was~100 μM, its effect was found to be significant at 6 hrs, and theranekron decreased the cell viability in all celllines without specificity in respect to the increasing concentrations. Additionally, a significantly increased GFPaccumulation was detected in the autophagosomes of the cells treated with theranekron compared to nontreated cells, indicating the presence of autophagy.Conclusion: These findings were confirmed by LC3-I to LC3-II conversion with the western blot analysis.The data of ethanol experiments; however, demonstrated that ethanol also induced a cytotoxic effect andautophagic cell death. Our results suggested that theranekron results in cell death and stimulate autophagyprocess, but it is not specific for cancer cells since it represented similar results on non-cancer control cells.Moreover, the effect of theranekron on cell death might mostly occur through alcohol in which it is extracted.
AU  - Yenigun, Vildan Betul
AU  - Acar, Mustafa Said
AU  - TASTEKIN, DIDEM
AU  - UCAR, Vasfiye Betul
AU  - Kaplan, M. Burak
AU  - Acar, Hasan
AU  - azzawri, Ali
DO  - 10.30714/j-ebr.2021267971
PY  - 2021
JO  - Experimental Biomedical Research
VL  - 4
IS  - 2
SN  - 2618-6454
SP  - 89
EP  - 98
DB  - TRDizin
UR  - http://search/yayin/detay/424350
ER  -