Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats

KÜÇÜKLER, Sefa; AKTAS, SINAN; Hanedan, Başak; ÇAĞLAYAN, Cüneyt; KANDEMİR, Fatih Mehmet

Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats

Fatih Mehmet KANDEMİR, (Atatürk Üniversitesi, Veteriner Fakültesi İlaç, Bölümü biyokimya, Erzurum, TÜRKİYE)

Başak HANEDAN, (Atatürk Üniversitesi, Veteriner Fakültesi İlaç, İçişleri Bakanlığı İlaç, Erzurum, TÜRKİYE)

SİNAN AKTAŞ, (Atatürk Üniversitesi, Veteriner Fakültesi İlaç, İçişleri Bakanlığı İlaç, Erzurum, TÜRKİYE)

Sefa KÜÇÜKLER, (Atatürk Üniversitesi, Veteriner Fakültesi İlaç, Bölümü biyokimya, Erzurum, TÜRKİYE)

Cüneyt ÇAĞLAYAN (Bingöl Üniversitesi, Veteriner Fakültesi İlaç, Biyokimya Anabilim Dalı, Bingöl, TÜRKİYE)

Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi

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Yıl: 2020 Cilt: 34 Sayı: 3 Sayfa Aralığı: 147 - 151 Metin Dili: İngilizce İndeks Tarihi: 19-06-2021

Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats

Öz:

Cisplatin treatment in patients with solid tumor is common, but a major factor limiting its use isnephrotoxic effects. Thus, this study was performed to investigate the effects of rutin on oxidativestress, apoptotic protein caspase-3 and anti-apoptotic protein Bcl-3 levels in nephrotoxicity causedby cisplatin in rats. The rats were randomly separated into three groups (7 rats in each group).Group I was treated with physiological saline. Group II was treated with cisplatin intraperitoneallyon the tenth day of the study. Group III was treated with rutin 150 mg/kg orally for 14 days pluscisplatin 10 mg/kg intraperitoneally on the tenth day of the study. The kidneys and blood sampleswere collected for the measurement of urea, creatinine, malondialdehyde (MDA), glutathione(GSH), superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), caspase-3 andB-cell lymphoma protein 3 (Bcl-3) levels. Single dose cisplatin treatment in the rats caused thekidney injury. Rutin alleviated nephrotoxicity by significantly increasing antioxidant activity, anddecreasing MDA, caspase-3 and Bcl-3 levels (P<0.001). The present study suggested that rutincould decrease oxidative stress by enhancing antioxidant activity and nephrotoxicity caused bycisplatin by decreasing apoptotic pathways.

Anahtar Kelime:

Ratlarda Sisplatinden Kaynaklanan Nefrotoksisite Üzerine Rutinin İyileştirici Etkileri

Öz:

Solid tümörü olan hastalarda sisplatin tedavisi yaygındır, fakat kullanımını sınırlandıran önemli bir faktör nefrotoksik etkileridir. Dolayısıyla bu çalışma ratlarda sisplatinden kaynaklanan nefrotoksisitede oksidatif stres, apoptotik protein kaspaz-3 ve antiapoptotik protein Bcl-3 düzeyleri üzerine rutinin etkilerini değerlendirmek amacıyla yapıldı. Ratlar rastgele 3 gruba ayrıldı (her grupta 7 adet rat). Grup I’e serum fizyolojik uygulandı. Grup II’ye çalışmanın 10. gününde periton içi sisplatin uygulandı. Grup III’e 14 gün boyunca 150 mg/kg ağızdan rutin ve çalışmanın 10. gününde 10 mg/kg periton içi sisplatin uygulandı. Üre, kreatinin, malondialdehit (MDA), glutatyon (GSH), süperoksit dismutaz (SOD), katalaz, glutatyon peroksidaz (GSH-Px), kaspaz-3 ve B-hücre lenfoma protein 3 (Bcl-3) düzeylerinin ölçülmesi için böbrekler ve kan örnekleri toplandı. Ratlarda tek doz sisplatin uygulanması böbrek hasarına neden oldu. Rutin, antioksidant aktiviteyi artırarak ve MDA, kaspaz-3 ve Bcl-3 düzeylerini azaltarak nefrotoksisiteyi önemli düzeyde azalttı (P<0.001). Mevcut çalışma rutinin antioksidant aktiviteyi artırarak oksidatif stresi ve apoptotik yolakları azaltarak sisplatinden kaynaklanan nefrotoksisiteyi hafifletebileceğini gösterdi.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KANDEMİR F, Hanedan B, AKTAS S, KÜÇÜKLER S, ÇAĞLAYAN C (2020). Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. , 147 - 151.
Chicago	KANDEMİR Fatih Mehmet,Hanedan Başak,AKTAS SINAN,KÜÇÜKLER Sefa,ÇAĞLAYAN Cüneyt Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. (2020): 147 - 151.
MLA	KANDEMİR Fatih Mehmet,Hanedan Başak,AKTAS SINAN,KÜÇÜKLER Sefa,ÇAĞLAYAN Cüneyt Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. , 2020, ss.147 - 151.
AMA	KANDEMİR F,Hanedan B,AKTAS S,KÜÇÜKLER S,ÇAĞLAYAN C Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. . 2020; 147 - 151.
Vancouver	KANDEMİR F,Hanedan B,AKTAS S,KÜÇÜKLER S,ÇAĞLAYAN C Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. . 2020; 147 - 151.
IEEE	KANDEMİR F,Hanedan B,AKTAS S,KÜÇÜKLER S,ÇAĞLAYAN C "Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats." , ss.147 - 151, 2020.
ISNAD	KANDEMİR, Fatih Mehmet vd. "Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats". (2020), 147-151.

APA	KANDEMİR F, Hanedan B, AKTAS S, KÜÇÜKLER S, ÇAĞLAYAN C (2020). Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 34(3), 147 - 151.
Chicago	KANDEMİR Fatih Mehmet,Hanedan Başak,AKTAS SINAN,KÜÇÜKLER Sefa,ÇAĞLAYAN Cüneyt Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 34, no.3 (2020): 147 - 151.
MLA	KANDEMİR Fatih Mehmet,Hanedan Başak,AKTAS SINAN,KÜÇÜKLER Sefa,ÇAĞLAYAN Cüneyt Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, vol.34, no.3, 2020, ss.147 - 151.
AMA	KANDEMİR F,Hanedan B,AKTAS S,KÜÇÜKLER S,ÇAĞLAYAN C Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2020; 34(3): 147 - 151.
Vancouver	KANDEMİR F,Hanedan B,AKTAS S,KÜÇÜKLER S,ÇAĞLAYAN C Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2020; 34(3): 147 - 151.
IEEE	KANDEMİR F,Hanedan B,AKTAS S,KÜÇÜKLER S,ÇAĞLAYAN C "Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats." Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 34, ss.147 - 151, 2020.
ISNAD	KANDEMİR, Fatih Mehmet vd. "Ameliorating Effects of Rutin on Nephrotoxicity Caused by Cisplatin in Rats". Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 34/3 (2020), 147-151.