TY  - JOUR
TI  - Apoptotic Effects of Beta-Carotene, Alpha-Tocopherol and Ascorbic Acid on PC-3 Prostate Cancer Cells
AB  - Prostate cancer (PC) is one of the most commonly diagnosed cancer types being the second major reason of cancerassociateddeath in male particularly over the age of 50. Accumulating scientific evidences suggest the role oxidativestress and reactive oxygen species (ROS) in prostate cancer. A variety of factors including carcinogenic molecules, infectiousdiseases and toxic compounds can induce ROS production which turns into a strong contribution to the disturbedhomeostasis and genetic mutation. Antioxidants can decrease the negative effects of ROS in vitro. Vitamin C (Ascorbicacid, Asc), vitamin A (beta carotenoids and retinoids, β-Crt) and vitamin E (alpha tocopherol, α-Toc) play an important rolein inhibition of oxidative stress and diminishing of free radicals in the body. The aim of this study was to determine theanticancer effect of α-Toc, β-Crt and Asc on PC-3 prostate cancer cells in vitro. This was carried out by cell proliferation, ROSand Lipid Peroxidation assay, caspase-3 and propidium iodide staining experiments. The findings suggest that these agentsbehave as prooxidant by lowering cell viability and increasing the production of ROS and LPO in prostate cancer. Theseoxidants induce apoptosis as supported by caspase-3 (the enzyme playing key role in programmed cell death) staining bydisplaying a marked increase in the expression level of caspase-3 enzyme.
AU  - AYNA, ADNAN
DO  - 10.15671/hjbc.519212
PY  - 2020
JO  - Hacettepe Journal of Biology and Chemistry
VL  - 48
IS  - 3
SN  - 1303-5002
SP  - 211
EP  - 218
DB  - TRDizin
UR  - http://search/yayin/detay/429182
ER  -