TY  - JOUR
TI  - LEF1 Induces DHRS2 Gene Expression in Human Acute Leukemia Jurkat T-Cells
AB  - Objective: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressivedisease resulting from the accumulation of genetic changes that affectthe development of T-cells. The precise role of lymphoid enhancerbinding factor 1 (LEF1) in T-ALL has been controversial since bothoverexpression and inactivating LEF1 mutations have been reportedto date. Here, we investigate the potential gene targets of LEF1 in theJurkat human T-cell leukemia cell line.Materials and Methods: We used small interfering RNA (siRNA)technology to knock down LEF1 in Jurkat cells and then comparedthe gene expression levels in the LEF1 knockdown cells with nontargeting siRNA-transfected and non-transfected cells by employingmicroarray analysis.Results: We identified DHRS2, a tumor suppressor gene, as the mostsignificantly downregulated gene in LEF1 knockdown cells, and wefurther confirmed its downregulation by real-time quantitativepolymerase chain reaction (qRT-PCR) in mRNA and at protein level bywestern blotting.Conclusion: Our results revealed that DHRS2 is positively regulated byLEF1 in Jurkat cells, which indicates the capability of LEF1 as a tumorsuppressor and, together with previous reports, suggests that LEF1exhibits a regulatory role in T-ALL via not only its oncogenic targetsbut also tumor suppressor genes.
AU  - Salman Yaylaz, Burcu
AU  - EMRENCE, ZELIHA
AU  - Sariman, Melda
AU  - ABACI, NESLIHAN
AU  - Gulec, Cagri
AU  - Sırma Ekmekci, Sema
AU  - EKMEKCI, CUMHUR GOKHAN
DO  - 10.4274/tjh.galenos.2020.2020.0144
PY  - 2020
JO  - Turkish Journal of Hematology
VL  - 37
IS  - 4
SN  - 1300-7777
SP  - 226
EP  - 233
DB  - TRDizin
UR  - http://search/yayin/detay/429849
ER  -