Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

Turan, Kadir; ÇAĞLAYAN, Elif

Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

Elif ÇAĞLAYAN, (Marmara Üniversitesi, Sağlık Bilimleri Enstitüsü, İstanbul, Türkiye)

Kadir TURAN (Marmara Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Anabilim Dalı, İstanbul, Türkiye)

Turkish Journal of Biology

0 0

Yıl: 2021 Cilt: 45 Sayı: 1 Sayfa Aralığı: 88 - 103 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

Öz:

Influenza A viruses frequently change their genetic characteristics, which leads to the emergence of new viruses. Consequently, elucidation of the relationship between influenza A virus and host cells has a great importance to cope with viral infections. In this study, it was aimed to determine expression profiles of interferon response genes in human embryonic kidney 293 (HEK293) cells infected with human (A/WSN-H1N1) and avian influenza A viruses (duck/Pennsylvania/10218/84/H5N2) or transfected with plasmids encoding viral RdRP subunits and, to obtain clues about the genes that may be important for the viral pathogenesis. The HEK293 cells cultured in a 12-well plate were infected with influenza A viruses or transfected with plasmids encoding viral polymerase. Total RNA extraction and cDNA preparation were carried out with commercial kits. Qiagen 96-well-RT2 Profiler PCR Array plates designated for interferons response genes were used for quantitation of the transcripts. The relative quantities of transcripts were normalized with STAT3 gen, and the results were evaluated. Quantitative RT-PCR results showed that there are substantial differences of the interferon response gene transcription in cells infected with viruses or transfected with plasmids. A higher number of interferon-related genes were found to be downregulated in the cells infected with DkPen compared to WSN. On the other hand, significant differences in the expression profiles of interferon response genes were observed in the cells expressing viral PA protein. In particular, avian influenza PA protein was found to cause more aggressive changes on the transcript levels. Human and avian influenza A viruses cause a substantial change in interferon response gene expression in HEK293 cells. However, a higher number of genes were downregulated in the cells infected with avian influenza DkPen compared to WSN. It has been also concluded that the viral PA protein is one of the important viral factors affecting the transcript level of host genes.

Anahtar Kelime: interferon response Influenza A viruses influenza RdRP PCR array

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Amiot L, Vu N, Samson M (2014). Immunomodulatory properties of HLA-G in infectious diseases. Journal of Immunology Research 2014: 298569. doi: 10.1155/2014/298569.
Beare AS, Webster RG (1991). Replication of avian influenza viruses in humans. Archives of Virology 119 (1-2): 37-42. doi: 10.1007/ BF01314321.
De Vlugt C, Sikora D, Pelchat M (2018). Insight into influenza: a virus cap-snatching. Viruses 10 (11): 641. doi: 10.3390/v10110641.
Dias A, Bouvier D, Crepin T, McCarthy A, Hart D et al. (2009). The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit. Nature 458 (7240): 914-918. doi: 10.1038/nature07745.
Fodor E (2013). The RNA polymerase of influenza A virus: mechanisms of viral transcription and replication. Acta Virologica 57 (2): 113-122. doi: 10.4149/av_2013_02_113.
Garcia MA, Gil J, Ventoso I, Guerra S, Domingo E et al. (2006). Impact of protein kinase PKR in cell biology: from antiviral to antiproliferative action. Microbiology and Molecular Biology Reviews 70 (4): 1032-1060. doi: 10.1128/MMBR.00027-06.
Gelmez N (2019). Investigation of the relationship between influenza A virus RNA polymerase and human actin beta protein. MSc, Marmara University, İstanbul, Turkey.
Govindarajan R, Duraiyan J, Kaliyappan K, Palanisamy M (2012). Microarray and its applications. Journal of Pharmacy & BioAllied Sciences 4 (6): 310-312. doi: 10.4103/0975- 7406.100283.
Graef KM, Vreede FT, Lau Y, McCall A, Carr S et al. (2010). The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with the mitochondrial antiviral signaling protein and inhibiting expression of beta interferon. Journal of Virology 84 (17): 8433-8445. doi: 10.1128/JVI.00879-10.
Hatta M, Gao P, Halfmann P, Kawaoka Y (2001). Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science 293 (5536): 1840-1842. doi: 10.1126/science.1062882.
Herold S, Becker C, Ridge KM, Budinger GR (2015). Influenza virus-induced lung injury: pathogenesis and implications for treatment. European Respiratory Journal 45 (5): 1463-1478. doi: 10.1183/09031936.00186214.
Holzinger D, Jorns C, Stertz S, Boisson-Dupuis S, Thimme R et al. (2007). Induction of MxA gene expression by influenza A virus requires type I or type III interferon signaling. Journal of Virology 81 (14): 7776-7785. doi: 10.1128/JVI.00546-06.
Kamiki H, Matsugo H, Kobayashi T, Ishida H, Takenaka-Uema A et al. (2018). A PB1-K577E mutation in H9N2 influenza virus increases polymerase activity and pathogenicity in mice. Viruses 10 (11): 653. doi: 10.3390/v10110653.
Kawaoka Y, Neumann G (2012). Influenza viruses: an introduction. Methods in Molecular Biology 865: 1-9. doi: 10.1007/978-1- 61779-621-0_1.
LeBouder F, Khoufache K, Menier C, Mandouri Y, Keffous M et al. (2009). Immunosuppressive HLA-G molecule is upregulated in alveolar epithelial cells after influenza A virus infection. Human Immunology 70 (12): 1016-1019. doi: 10.1016/j. humimm.2009.07.026.
Mehle A, Doudna JA (2010). A host of factors regulating influenza virus replication. Viruses 2 (2): 566-573. doi: 10.3390/ v2020566.
Melchjorsen J, Kristiansen H, Christiansen R, Rintahaka J, Matikainen S et al. (2009). Differential regulation of the OASL and OAS1 genes in response to viral infections. Journal of Interferon & Cytokine Research 29 (4): 199-207. doi: 10.1089/ jir.2008.0050.
Minamoto S, Ikegame K, Ueno K, Narazaki M, Naka T et al. (1997). Cloning and functional analysis of new members of STAT induced STAT inhibitor (SSI) family: SSI-2 and SSI-3. Biochemical and Biophysical Research Communications 237 (1): 79-83. doi: 10.1006/bbrc.1997.7080.
Moncorgé O, Mura M, Barclay WS (2010). Evidence for avian and human host cell factors that affect the activity of influenza virus polymerase. Journal of Virology 84 (19): 9978-9986. doi: 10.1128/JVI.01134-10.
Nakajima K (2003). The mechanism of antigenic shift and drift of human influenza virus. Nihon Rinsho. Japanese Journal of Clinical Medicine 61 (11): 1897-1903.
Niwa H, Yamamura K, Miyazaki J (1991). Efficient selection for highexpression transfectants with a novel eukaryotic vector. Gene 108 (2): 193-199. doi: 10.1016/0378-1119(91)90434-d.
Park KJ, Song MS, Kim EH, Kwon H, Baek YH et al. (2015). Molecular characterization of mammalian-adapted Koreantype avian H9N2 virus and evaluation of its virulence in mice. Journal of Microbiology 53 (8): 570-577. doi: 10.1007/s12275- 015-5329-4.
Pham PTV, Turan K, Nagata K, Kawaguchi A (2018). Biochemical characterization of avian influenza viral polymerase containing PA or PB2 subunit from human influenza A virus. Microbes Infection 20 (6): 353-359. doi: 10.1016/j.micinf.2018.04.003.
Pothlichet J, Chignard M, Si-Tahar M (2008). Cutting edge: innate immune response triggered by influenza A virus is negatively regulated by SOCS1 and SOCS3 through a RIG-I/IFNAR1- dependent pathway. Journal of Immunology 180 (4): 2034- 2038. doi: 10.4049/jimmunol.180.4.2034.
Qiu Y, Krug RM (1994). The influenza virus NS1 protein is a poly(A)- binding protein that inhibits nuclear export of mRNAs containing poly(A). Journal of Virology 68 (4): 2425-2432.
Qiu Y, Nemeroff M, Krug RM (1995). The influenza virus NS1 protein binds to a specific region in human U6 snRNA and inhibits U6-U2 and U6-U4 snRNA interactions during splicing. RNA 1 (3): 304-316.
Sen GC, Peters GA (2007). Viral stress-inducible genes. Advances in Virus Research 70: 233-263. doi: 10.1016/S0065- 3527(07)70006-4.
Skehel JJ, Wiley DC (2000). Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. Annual Review of Biochemistry 69: 531-69. doi: 10.1146/annurev. biochem.69.1.531.
Stubbs TM, Te Velthuis AJ (2014). The RNA-dependent RNA polymerase of the influenza A virus. Future Virology 9 (9): 863-876. doi: 10.2217/fvl.14.66.
Takahashi T, Maruyama M, Ishida H, Kiso M, Kawaoka Y et al. (2009). Identification of amino acid residues of influenza A virus H3 HA contributing to the recognition of molecular species of sialic acid. FEBS Letters 583 (19): 3171-3174. doi: 10.1016/j.febslet.2009.08.037.
Turan K, Mibayashi M, Sugiyama K, Saito S, Numajiri A et al. (2004). Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome. Nucleic Acids Research 32 (2): 643-652. doi: 10.1093/ nar/gkh192.
Turan K, Nagata K, Kuru A (1996). Antiviral effect of Sanicula europaea L. leaves extract on influenza virus-infected cells. Biochemical and Biophysical Research Communications 225 (1): 22-26. doi: 10.1006/bbrc.1996.1125.
Tyner JW, Uchida O, Kajiwara N, Kim EY, Patel AC et al. (2005). CCL5-CCR5 interaction provides antiapoptotic signals for macrophage survival during viral infection. Nature Medicine 11 (11): 1180-1187. doi: 10.1038/nm1303.
Walsh D, Mathews MB, Mohr I (2013). Tinkering with translation: protein synthesis in virus-infected cells. Cold Spring Harbor Perspectives in Biology 5 (1): a012351. doi: 10.1101/ cshperspect.a012351.
Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y (1992). Evolution and ecology of influenza A viruses. Microbiology Reviews 56 (1): 152-79.
Zhang H, Hale BG, Xu K, Sun B (2013). Viral and host factors required for avian H5N1 influenza A virus replication in mammalian cells. Viruses 5 (6): 1431-1446. doi: 10.3390/v5061431.
Zimmermann P, Manz B, Haller O, Schwemmle M, Kochs G (2011). The viral nucleoprotein determines Mx sensitivity of influenza A viruses. Journal of Virology 85 (16): 8133-8140. doi: 10.1128/ JVI.00712-11.

APA	ÇAĞLAYAN E, Turan K (2021). Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. , 88 - 103.
Chicago	ÇAĞLAYAN Elif,Turan Kadir Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. (2021): 88 - 103.
MLA	ÇAĞLAYAN Elif,Turan Kadir Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. , 2021, ss.88 - 103.
AMA	ÇAĞLAYAN E,Turan K Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. . 2021; 88 - 103.
Vancouver	ÇAĞLAYAN E,Turan K Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. . 2021; 88 - 103.
IEEE	ÇAĞLAYAN E,Turan K "Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase." , ss.88 - 103, 2021.
ISNAD	ÇAĞLAYAN, Elif - Turan, Kadir. "Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase". (2021), 88-103.

APA	ÇAĞLAYAN E, Turan K (2021). Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. Turkish Journal of Biology, 45(1), 88 - 103.
Chicago	ÇAĞLAYAN Elif,Turan Kadir Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. Turkish Journal of Biology 45, no.1 (2021): 88 - 103.
MLA	ÇAĞLAYAN Elif,Turan Kadir Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. Turkish Journal of Biology, vol.45, no.1, 2021, ss.88 - 103.
AMA	ÇAĞLAYAN E,Turan K Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. Turkish Journal of Biology. 2021; 45(1): 88 - 103.
Vancouver	ÇAĞLAYAN E,Turan K Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase. Turkish Journal of Biology. 2021; 45(1): 88 - 103.
IEEE	ÇAĞLAYAN E,Turan K "Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase." Turkish Journal of Biology, 45, ss.88 - 103, 2021.
ISNAD	ÇAĞLAYAN, Elif - Turan, Kadir. "Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase". Turkish Journal of Biology 45/1 (2021), 88-103.