Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method
Yıl: 2021 Cilt: 17 Sayı: 1 Sayfa Aralığı: 108 - 118 Metin Dili: İngilizce DOI: 10.22392/actaquatr.762038 İndeks Tarihi: 05-08-2021
Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method
Öz: Mussels (Mytilus galloprovincialis) are aquatic organisms that can accumulate all the factors that pollute the fresh andsaltwater environment. Although the Dardanelles has been exposed to environmental pollution for many years, it is also animportant region for crustaceans. We aimed to investigate the histopathological changes in the liver parenchyma which is animportant gland of the digestive system by feeding these mussels to rats. Twenty-four male Wistar albino were used in thestudy. The first group (control): fed with standard rat feed, the second group (experiment 1): 4/5 mussel + 1/5 standard ratfeed daily; third group (experiment 2): 4/5 mussel + 1/5 standard rat feed every other day, the fourth group (experiment 3):groups were formed with 4/5 mussel + 1/5 standard rat feed every three days. All liver tissue samples taken from theexperimental and control groups were stained with hematoxylin-eosin, and immuohitochemically staining inflammatorymarker TNF-α and NF-κB after routine histopathological follow-up and analyzed with a light microscope image analysissystem. It was observed that mononuclear cells caused inflammation of portal areas, increased sinusoidal dilatation andcongestion and degeneration due to vacuolization in hepatocytes in the liver parenchyma of mussel-fed rats. Besides,immunohistochemical staining, TNF-α, and NF-κB immunoreactivity were observed in the liver cells of especially in thesecond group of rats. As a result, it has been shown that the consumption of mussels obtained and marketed withoutconsidering environmental pollution may trigger important digestive system organs of liver diseases.
Anahtar Kelime: Kirlenmiş Alanlardan Toplanan Akdeniz Midyesi'nin Ratlarda İmmünohistokimyasal Metotla Hepatotoksik Etkisi
Öz: Midye (Mytilus galloprovincialis), tatlı ve tuzlu su ortamını kirleten tüm faktörleri biriktirebilen sucul organizmalardır. Çanakkale Boğazı uzun yıllar boyunca çevre kirliliğine maruz kalsada, kabuklular için de önemli bir bölgedir. Bu midyelerin sindirim sisteminin önemli bir bezi olan karaciğer parankimindeki histopatolojik etkilerini araştırmayı amaçladık. Çalışmada 24 adet erkek Wistar albino türü rat kullanıldı. Birinci grup (kontrol): standart sıçan yemi ile beslendi, ikinci grup (deney 1): hergün 4/5 midye + 1/5 standart sıçan yemi; üçüncü grup (deney 2): 4/5 midye + 1/5 standart sıçan yemi 2 günde bir, dördüncü grup (deney 3): gruplar 4/5 midye + 1/5 standart sıçan yemi ile her üç günde bir oluşturuldu. Deney ve kontrol gruplarından alınan tüm karaciğer doku örnekleri, histopatolojik takipten sonra rutin hematoksilin-eozin ve enflamatuar belirteç olan TNF-α ve NF-κB ile immünohistokimyasal boyama yapıldı ve ışık mikroskobu görüntü analiz sistemi ile analiz edildi. Mononükleer hücrelerin portal alanlarda yangıya sebep olduğu, karaciğer parankimindeki hepatositlerde vakuolizasyonun dejenerasyon sonucu meydana geldiği, santral ven ve sinüzoidal dilatasyon ve konjesyonun olduğu gözlemlendi. Ek olarak, özellikle ikinci sıçan grubunun karaciğer hücrelerinde immünohistokimyasal boyama, TNF-α ve NFκB şiddetli immünoreaktivitesi gözlendi. Sonuç olarak, çevre kirliliği dikkate alınmadan elde edilen ve pazarlanan midye tüketiminin sindirim sisteminin önemli bir organı olan karaciğer hastalıklarını tetikleyebileceği gösterilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | İRKİN L, ÇAKINA S, ÖZDEMİR İ, ÖZTÜRK Ş (2021). Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. , 108 - 118. 10.22392/actaquatr.762038 |
| Chicago | İRKİN LATİFE CEYDA,ÇAKINA SUAT,ÖZDEMİR İlhan,ÖZTÜRK Şamil Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. (2021): 108 - 118. 10.22392/actaquatr.762038 |
| MLA | İRKİN LATİFE CEYDA,ÇAKINA SUAT,ÖZDEMİR İlhan,ÖZTÜRK Şamil Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. , 2021, ss.108 - 118. 10.22392/actaquatr.762038 |
| AMA | İRKİN L,ÇAKINA S,ÖZDEMİR İ,ÖZTÜRK Ş Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. . 2021; 108 - 118. 10.22392/actaquatr.762038 |
| Vancouver | İRKİN L,ÇAKINA S,ÖZDEMİR İ,ÖZTÜRK Ş Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. . 2021; 108 - 118. 10.22392/actaquatr.762038 |
| IEEE | İRKİN L,ÇAKINA S,ÖZDEMİR İ,ÖZTÜRK Ş "Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method." , ss.108 - 118, 2021. 10.22392/actaquatr.762038 |
| ISNAD | İRKİN, LATİFE CEYDA vd. "Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method". (2021), 108-118. https://doi.org/10.22392/actaquatr.762038 |
| APA | İRKİN L, ÇAKINA S, ÖZDEMİR İ, ÖZTÜRK Ş (2021). Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. Acta Aquatica Turcica, 17(1), 108 - 118. 10.22392/actaquatr.762038 |
| Chicago | İRKİN LATİFE CEYDA,ÇAKINA SUAT,ÖZDEMİR İlhan,ÖZTÜRK Şamil Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. Acta Aquatica Turcica 17, no.1 (2021): 108 - 118. 10.22392/actaquatr.762038 |
| MLA | İRKİN LATİFE CEYDA,ÇAKINA SUAT,ÖZDEMİR İlhan,ÖZTÜRK Şamil Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. Acta Aquatica Turcica, vol.17, no.1, 2021, ss.108 - 118. 10.22392/actaquatr.762038 |
| AMA | İRKİN L,ÇAKINA S,ÖZDEMİR İ,ÖZTÜRK Ş Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. Acta Aquatica Turcica. 2021; 17(1): 108 - 118. 10.22392/actaquatr.762038 |
| Vancouver | İRKİN L,ÇAKINA S,ÖZDEMİR İ,ÖZTÜRK Ş Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method. Acta Aquatica Turcica. 2021; 17(1): 108 - 118. 10.22392/actaquatr.762038 |
| IEEE | İRKİN L,ÇAKINA S,ÖZDEMİR İ,ÖZTÜRK Ş "Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method." Acta Aquatica Turcica, 17, ss.108 - 118, 2021. 10.22392/actaquatr.762038 |
| ISNAD | İRKİN, LATİFE CEYDA vd. "Effect of Mediterranean Mussels (Mytilus galloprovincialis) From Polluted Areas on Hepatotoicity in Rats by Immunuhistochemical Method". Acta Aquatica Turcica 17/1 (2021), 108-118. https://doi.org/10.22392/actaquatr.762038 |
