TY  - JOUR
TI  - Helicobacter-stimulated IL-10-producing B cells suppress differentiation of lipopolysaccharide/Helicobacter felis-activated stimulatory dendritic cells
AB  - Regulatory B cells (Bregs) produce antiinflammatory cytokines and inhibits proinflammatory response. Recently,immunosuppressive roles of Bregs in the effector functions of dendritic cells (DCs) were demonstrated. However, cross talk betweenBregs and DCs in Helicobacter infection remains unknown. Here, we showed that direct stimulation of bone marrow-derived DCs (BMDCs) with Helicobacter felis (H. felis) antigen upregulates their CD86 surface expression and causes the production of interleukin-6(IL-6), tumor necrosis factor alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). Furthermore, prestimulation of DCswith supernatants derived from both Helicobacter-stimulated IL-10– B (Hfstim-IL-10– B) or IL-10+ B (Hfstim-IL-10+) cells suppresses thesecretion of TNF-α and IL-6, but does not affect the expression of CD86 and secretion of IL-12 by lipopolysaccharide (LPS) or H. felisactivated BM-DCs. Remarkably, soluble factors secreted by Hfstim-IL-10– B cells, but not by Hfstim-IL-10+ B cells, suppress the secretionof IL-10 by BM-DCs upon subsequent LPS stimulation. In contrast, prestimulation with BM-DCs with supernatants of Hfstim-IL-10+ Bcells before H. felis antigen stimulation induces significantly their IL-10 production. Collectively, our data indicated that prestimulationwith soluble factors secreted by Hfstim-IL-10+ B cells, DCs exhibit a tolerogenic phenotype in response to LPS or Helicobacter antigen bysecreting high levels of IL-10, but decreased levels of IL-6 and TNF-α.
AU  - Sayi Yazgan, Ayca
AU  - Altunoz, Dogus
DO  - 10.3906/biy-2012-11
PY  - 2021
JO  - Turkish Journal of Biology
VL  - 45
IS  - 2
SN  - 1300-0152
SP  - 214
EP  - 224
DB  - TRDizin
UR  - http://search/yayin/detay/434804
ER  -