TY  - JOUR
TI  - Arid4b physically interacts with Tfap2c in mouse embryonic stem cells
AB  - Precise regulation of gene expression is required for embryonic stem cell (ESC) differentiation. Transcription factor (TF)networks coordinate the balance of pluripotency and differentiation in response to extracellular and intracellular signals. Chromatinfactors work alongside TFs to achieve timely regulation of gene expression for differentiation process. Our previous studies showed thata member of the Sin3a corepressor complex, Arid4b, is critical for proper mouse ESC differentiation into mesoderm and endoderm. Wefound elevated histone 3 lysine 27 acetylation (H3K27Ac) in a subset of genomic loci in meso/endoderm directed arid4bΔ cells, coincidentwith their derepression. We reasoned that Sin3a complex may be required for the suppression of these genes during differentiation. Toidentify TFs that might cooperate with Arid4b for this function, we found consensus TF binding sequences enriched in H3K27Acelevated regions in arid4bΔ cells. Of these candidate TFs, we validated expression of Bach1, Ddit3, Prrx2, Znf354c and Tfap2c in mESCs.We then demonstrated a physical interaction between Arid4b and Tfap2c in mESCs using endogenous coimmunoprecipitation andproximity ligation assay experiments. Our results point to a role of Arid4b in the Sin3a complex in repression of a subset of Tfap2cregulated genes during meso/endoderm differentiation.
AU  - Keskin, Ezgi Gül
AU  - TERZI ÇIZMECIOGLU, NIHAL
AU  - Huang, Jialiang
DO  - 10.3906/biy-2010-67
PY  - 2021
JO  - Turkish Journal of Biology
VL  - 45
IS  - 2
SN  - 1300-0152
SP  - 162
EP  - 170
DB  - TRDizin
UR  - http://search/yayin/detay/434807
ER  -