Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A

Ahmet ÖZKAYA, (Adıyaman Üniversitesi, Fen-Edebiyat Fakültesi, Kimya Bölümü, Adıyaman, Türkiye)
Zafer ŞAHİN, (Karadeniz Teknik Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Trabzon, Türkiye)
Yunus ŞAHİN, (Adıyaman Üniversitesi, Fen-Edebiyat Fakültesi, Kimya Bölümü, Adıyaman, Türkiye)
Özgür BULMUŞ, (Balıkesir Üniversitesi, Sağlık Bilimleri Fakültesi, Fizik Tedavi ve Rehabilitasyon Bölümü, Balıkesir, Türkiye)
Miraç UÇKUN, (Adıyaman Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, Adıyaman, Türkiye)
Ertan YOLOĞLU (Adıyaman Üniversitesi, Eğitim Fakültesi, Fen Bilimleri Eğitimi Bölümü, Adıyaman, Türkiye)
Adıyaman Üniversitesi Fen Bilimleri Dergisi
3 2
Yıl: 2021 Cilt: 11 Sayı: 1 Sayfa Aralığı: 73 - 86 Metin Dili: İngilizce DOI: 10.37094/adyujsci.782874 İndeks Tarihi: 29-07-2022

Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A

Öz:
We aimed to evaluate the effects of black carrot extract on testicular carboxylesterase (Ces)activity, malondialdehyde (MDA), glutathione S-transferases (GST) and reduced glutathione(GSH) in male rats exposed to bisphenol A (BPA). Adult Wistar albino male rats were dividedinto 4 groups as follows (n = 7/group): control, BPA, black carrot and BPA+Black carrot.Testicular Ces, MDA, GST and reduced GSH were analyzed by using a spectrophotometersystem. Testicular Ces activity was significantly lower only in the BPA group than the controlgroup (p < 0.001). Testicular MDA concentrations were higher only in the BPA group comparedwith the control group (p < 0.001). Reduced GSH level was higher in the black carrot andBPA+Black carrot groups than the control group (p < 0.05). GST activity was lower in the BPA group in comparison to the control group (p < 0.05). In the black carrot and BPA+Black carrotgroups, the GST activities were higher than the control group (p < 0.001). Our results showedBPA suppresses testicular detoxification activity and increases lipid peroxidation in rats. Weobserved that black carrot extract has a beneficial effect on the toxic and oxidative stress-relatedparameters caused by BPA exposure.
Anahtar Kelime: Rat Black carrot Testis Carboxylesterase Oxidative stress Bisphenol A

Bisfenol A’ya Maruz Kalan Sıçanlarda Siyah Havuç Ekstresinin Testis Karboksilesteraz Aktivitesi ve Oksidatif Stres Parametreleri Üzerindeki Etkilerinin Değerlendirilmesi

Öz:
Bu çalışmada Bisfenol A'ya (BPA) maruz kalan erkek sıçanlarda siyah havuç ekstraktının testiküler karboksilesteraz (Ces) aktivitesi, malondialdehit (MDA) düzeyi, indirgenmiş glutatyon (GSH) ve glutatyon S-transferaz (GST) aktiviteleri üzerine etkilerini değerlendirmeyi amaçladık. Yetişkin erkek Wistar albino sıçanlar kontrol, BPA, siyah havuç ve BPA+Siyah havuç olmak üzere 4 gruba ayrıldı (n = 7/grup). Testis dokusunda Ces, MDA, indirgenmiş GSH ve GST düzeyleri mikroplaka okuyucu spektrofotometre sistemi kullanılarak analiz edildi. Testis Ces aktivitesi sadece BPA grubunda kontrol grubuna kıyasla önemli düzeyde daha düşüktü (p < 0.001). Testis MDA konsantrasyonu sadece BPA grubunda kontrol grubuna kıyasla daha yüksekti (p < 0.001). İndirgenmiş GSH düzeyi siyah havuç ve BPA+Siyah havuç gruplarında kontrol grubuna kıyasla daha yüksekti (p < 0.05). GST aktivitesi BPA grubunda kontrol grubuna göre daha düşüktü (p < 0.05). Siyah havuç ve BPA + Siyah havuç gruplarında GST aktiviteleri kontrol grubuna kıyasla daha yüksekti (p <0.001). Sonuçlarımız, BPA’nın sıçan testis dokusunda detoksifikasyon aktivitesini baskıladığını ve lipit peroksidasyonunu arttırdığını gösterdi. Siyah havuç ekstraktının, BPA maruziyetinin neden olduğu toksik ve oksidatif stresle ilişkili parametreler üzerinde yaralı bir etkiye sahip olduğunu gözlemledik.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Özkaya A, Şahin Z, şahin y, Bulmus O, UÇKUN M, Yoloğlu E (2021). Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. , 73 - 86. 10.37094/adyujsci.782874
Chicago Özkaya Ahmet,Şahin Zafer,şahin yunus,Bulmus Ozgur,UÇKUN MIRAÇ,Yoloğlu Ertan Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. (2021): 73 - 86. 10.37094/adyujsci.782874
MLA Özkaya Ahmet,Şahin Zafer,şahin yunus,Bulmus Ozgur,UÇKUN MIRAÇ,Yoloğlu Ertan Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. , 2021, ss.73 - 86. 10.37094/adyujsci.782874
AMA Özkaya A,Şahin Z,şahin y,Bulmus O,UÇKUN M,Yoloğlu E Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. . 2021; 73 - 86. 10.37094/adyujsci.782874
Vancouver Özkaya A,Şahin Z,şahin y,Bulmus O,UÇKUN M,Yoloğlu E Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. . 2021; 73 - 86. 10.37094/adyujsci.782874
IEEE Özkaya A,Şahin Z,şahin y,Bulmus O,UÇKUN M,Yoloğlu E "Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A." , ss.73 - 86, 2021. 10.37094/adyujsci.782874
ISNAD Özkaya, Ahmet vd. "Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A". (2021), 73-86. https://doi.org/10.37094/adyujsci.782874
APA Özkaya A, Şahin Z, şahin y, Bulmus O, UÇKUN M, Yoloğlu E (2021). Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. Adıyaman Üniversitesi Fen Bilimleri Dergisi, 11(1), 73 - 86. 10.37094/adyujsci.782874
Chicago Özkaya Ahmet,Şahin Zafer,şahin yunus,Bulmus Ozgur,UÇKUN MIRAÇ,Yoloğlu Ertan Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. Adıyaman Üniversitesi Fen Bilimleri Dergisi 11, no.1 (2021): 73 - 86. 10.37094/adyujsci.782874
MLA Özkaya Ahmet,Şahin Zafer,şahin yunus,Bulmus Ozgur,UÇKUN MIRAÇ,Yoloğlu Ertan Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. Adıyaman Üniversitesi Fen Bilimleri Dergisi, vol.11, no.1, 2021, ss.73 - 86. 10.37094/adyujsci.782874
AMA Özkaya A,Şahin Z,şahin y,Bulmus O,UÇKUN M,Yoloğlu E Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. Adıyaman Üniversitesi Fen Bilimleri Dergisi. 2021; 11(1): 73 - 86. 10.37094/adyujsci.782874
Vancouver Özkaya A,Şahin Z,şahin y,Bulmus O,UÇKUN M,Yoloğlu E Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. Adıyaman Üniversitesi Fen Bilimleri Dergisi. 2021; 11(1): 73 - 86. 10.37094/adyujsci.782874
IEEE Özkaya A,Şahin Z,şahin y,Bulmus O,UÇKUN M,Yoloğlu E "Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A." Adıyaman Üniversitesi Fen Bilimleri Dergisi, 11, ss.73 - 86, 2021. 10.37094/adyujsci.782874
ISNAD Özkaya, Ahmet vd. "Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A". Adıyaman Üniversitesi Fen Bilimleri Dergisi 11/1 (2021), 73-86. https://doi.org/10.37094/adyujsci.782874
Sayfa Oluşturulma Tarihi
23-04-2024 19:4

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