Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress

Gülçin ÇETİN KILIÇASLAN ÇETİN KILIÇASLAN, (Bilecik Şeyh Edebali Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Bilecik, Türkiye)
Özge KAYGUSUZ, (Bilecik Şeyh Edebali Üniversitesi, Biyoteknoloji Uygulama ve Araştırma Merkezi, Bilecik, Türkiye)
Önder İDİL, (Amasya Üniversitesi, Eğitim Fakültesi, Temel Eğitim Bölümü, Amasya, Türkiye)
Cihan DARCAN (Bilecik Şeyh Edebali Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Bilecik, Türkiye)
Adıyaman Üniversitesi Fen Bilimleri Dergisi
3 0
Yıl: 2021 Cilt: 11 Sayı: 1 Sayfa Aralığı: 1 - 22 Metin Dili: İngilizce DOI: 10.37094/adyujsci.834522 İndeks Tarihi: 29-07-2022

Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress

Öz:
Adenosine 3′, 5′-monophosphate (cAMP) is an important signaling molecule. CRP, thereceptor protein of cAMP, acts as the 'main' regulator for transcription factors. The CRP-cAMPcomplex directly controls at least 500 promoters in Escherichia coli. In this study, the roles ofcyaA and crp genes in E. coli BW25113 strain under metal stress were investigated. The minimalinhibition concentration (MIC) and minimal cidal concentration (MCC) of 5 different metals (Zn,Ni, Co, Cd and Cu) on Escherichia coli BW25113 wild type, cyaA and crp mutant cells weredetermined. In addition, the effect of these metals on the survival of E. coli cyaA/crp mutants wasdetermined by growth and drop plate method. According to E. coli BW25113 wild type, cyaAmutant strain was observed sensitivity in all metals except copper, whereas resistance wasobserved in crp mutant strain only to zinc metal. The roles of the cyaA and crp genes in metalstress were confirmed by completing the genes on the plasmid. As a result, the roles of cyaA andcrp genes in metal resistance were revealed in this study.
Anahtar Kelime: crp Escherichia coli cyaA metal stress

Metal Stresinde Escherichia coli’nin cyaA/crp Genlerinin Rolünün Araştırılması

Öz:
Adenosin 3 ′, 5′-monofosfat (cAMP), önemli bir sinyal molekülüdür. cAMP’nin reseptör proteini olan CRP, transkripsiyon faktörleri için 'ana' düzenleyici olarak görev almaktadır. Escherichia coli'de CRP-cAMP kompleksi, en az 500 promotor'u doğrudan kontrol etmektedir. Bu çalışmada E. coli BW25113 suşunda cyaA ve crp genlerinin metal stresi altındaki rolleri araştırılmıştır. Escherichia coli BW25113 yabanıl tip, cyaA ve crp mutant hücrelerinin 5 farklı metale (Zn, Ni, Co, Cd ve Cu) karşı minimal inhibisyon konsantrasyonu (MİK) ve minimal sidal konsantrasyonu (MSK) belirlenmiştir. Ayrıca bu metal stresinin E. coli cyaA/crp mutantlarının yaşamı üzerine etkisi büyüme ve petri damlatma yöntemi ile belirlenmiştir. Çalışmada E. coli BW25113 yabanıl tipe göre, bakır hariç tüm metallerde cyaA mutant suşunda duyarlılık gözlenirken, sadece çinko metalinde crp mutant suşunda dirençlilik gözlenmiştir. cyaA ve crp genlerinin metal stresindeki rolleri, genlerin plazmit üzerinde tamamlanması yapılarak doğrulanmıştır. Sonuç olarak; bu çalışmada cyaA ve crp genlerinin metal direncindeki rolleri ortaya konulmuştur.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ÇETİN KILIÇASLAN G, KAYGUSUZ İZGÖRDÜ Ö, idil ö, Darcan C (2021). Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. , 1 - 22. 10.37094/adyujsci.834522
Chicago ÇETİN KILIÇASLAN Gülçin,KAYGUSUZ İZGÖRDÜ ÖZGE,idil önder,Darcan Cihan Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. (2021): 1 - 22. 10.37094/adyujsci.834522
MLA ÇETİN KILIÇASLAN Gülçin,KAYGUSUZ İZGÖRDÜ ÖZGE,idil önder,Darcan Cihan Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. , 2021, ss.1 - 22. 10.37094/adyujsci.834522
AMA ÇETİN KILIÇASLAN G,KAYGUSUZ İZGÖRDÜ Ö,idil ö,Darcan C Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. . 2021; 1 - 22. 10.37094/adyujsci.834522
Vancouver ÇETİN KILIÇASLAN G,KAYGUSUZ İZGÖRDÜ Ö,idil ö,Darcan C Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. . 2021; 1 - 22. 10.37094/adyujsci.834522
IEEE ÇETİN KILIÇASLAN G,KAYGUSUZ İZGÖRDÜ Ö,idil ö,Darcan C "Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress." , ss.1 - 22, 2021. 10.37094/adyujsci.834522
ISNAD ÇETİN KILIÇASLAN, Gülçin vd. "Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress". (2021), 1-22. https://doi.org/10.37094/adyujsci.834522
APA ÇETİN KILIÇASLAN G, KAYGUSUZ İZGÖRDÜ Ö, idil ö, Darcan C (2021). Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. Adıyaman Üniversitesi Fen Bilimleri Dergisi, 11(1), 1 - 22. 10.37094/adyujsci.834522
Chicago ÇETİN KILIÇASLAN Gülçin,KAYGUSUZ İZGÖRDÜ ÖZGE,idil önder,Darcan Cihan Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. Adıyaman Üniversitesi Fen Bilimleri Dergisi 11, no.1 (2021): 1 - 22. 10.37094/adyujsci.834522
MLA ÇETİN KILIÇASLAN Gülçin,KAYGUSUZ İZGÖRDÜ ÖZGE,idil önder,Darcan Cihan Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. Adıyaman Üniversitesi Fen Bilimleri Dergisi, vol.11, no.1, 2021, ss.1 - 22. 10.37094/adyujsci.834522
AMA ÇETİN KILIÇASLAN G,KAYGUSUZ İZGÖRDÜ Ö,idil ö,Darcan C Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. Adıyaman Üniversitesi Fen Bilimleri Dergisi. 2021; 11(1): 1 - 22. 10.37094/adyujsci.834522
Vancouver ÇETİN KILIÇASLAN G,KAYGUSUZ İZGÖRDÜ Ö,idil ö,Darcan C Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress. Adıyaman Üniversitesi Fen Bilimleri Dergisi. 2021; 11(1): 1 - 22. 10.37094/adyujsci.834522
IEEE ÇETİN KILIÇASLAN G,KAYGUSUZ İZGÖRDÜ Ö,idil ö,Darcan C "Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress." Adıyaman Üniversitesi Fen Bilimleri Dergisi, 11, ss.1 - 22, 2021. 10.37094/adyujsci.834522
ISNAD ÇETİN KILIÇASLAN, Gülçin vd. "Investigation of the Role of cyaA/crp Genes of Escherichia coli in Metal Stress". Adıyaman Üniversitesi Fen Bilimleri Dergisi 11/1 (2021), 1-22. https://doi.org/10.37094/adyujsci.834522
Sayfa Oluşturulma Tarihi
23-04-2024 19:4

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