Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method
Yıl: 2021 Cilt: 32 Sayı: 1 Sayfa Aralığı: 33 - 39 Metin Dili: İngilizce DOI: 10.35864/evmd.748570 İndeks Tarihi: 29-07-2022
Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method
Öz: Listeria genus is Gram-positive facultative intracellular bacteria that are found in the soil as saprophytesbut also contain species that have pathogenic effects in their last host. Listeria monocytogenes is the major pathogenin Listeria species that is responsible for the majority of Listeriosis cases in humans and animals. In this study, itwas aimed to investigate L. monocytogenes with histopathological and immunofluorescence (IF) methods in brainand liver tissues taken from sheep and cattle with clinical signs of suspicious Listeriosis. In the study, isolation andidentification of L. monocytogenes with cultural methods were also applied. The material of the study consistedof tissue samples from 16 sheep and 2 cattle. As a result of cultural analysis, L. monocytogenes was identifiedfrom 12 (66.6%) of cases. Within the framework of histopathological and macroscopic findings, 16 (88.8%) of 18cases with suspected Listeriosis were positive with the immunofluorescence method. These findings reveal that theimmunofluorescent staining method is a reliable and sensitive method in the diagnosis of Listeriosis.
Anahtar Kelime: Ruminantların ensefalitik ve septisemik listeriyozisinin immunfloresan metodla araştırılması
Öz: Listeria cinsi toprakta saprofit olarak bulunan ancak aynı zamanda son konaklarında patojenik etkilere sahip türler içeren Gram-pozitif fakültatif intraselüler bakterilerdir. Listeria monocytogenes, insanlarda ve hayvanlarda Listeriosis vakalarının çoğundan sorumlu olan Listeria türlerindeki başlıca patojendir. Bu çalışmada, Listeriyozis şüpheli klinik belirti gösteren koyun ve sığırlardan alınan beyin ve karaciğer dokularında L. monocytogenes’in histopatolojik ve immunfloresan metodlarla araştırılması amaçlanmıştır. Çalışmada L. monocytogenes’in kültürel yöntemlerle izolasyonu ve identifikasyonu da uygulanmıştır. Çalışmanın materyalini, 16 koyun ve iki sığıra ait doku örnekleri oluşturmuştur. Kültürel analiz sonucunda vakaların 12 (%66,6)’sinden L. monocytogenes identifiye edilmiştir. Histopatolojik ve makroskobik bulgular çerçevesinde Listeriyozis şüpheli 18 vakanın 16 (%88,8)’sı immunfloresan metodla pozitif tespit edilmiştir. Bu bulgular, immunfloresan boyama yönteminin Listeriyozisin teşhisinde güvenilir ve hassas bir yöntem olduğunu ortaya koymaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KARAKURT E, BÜYÜK F, DAĞ S, BEYTUT E, ÇELEBİ Ö, KARATAŞ Ö, NUHOĞLU H, MENDİL A, AKÇA D, YILDIZ A (2021). Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. , 33 - 39. 10.35864/evmd.748570 |
| Chicago | KARAKURT Emin,BÜYÜK Fatih,DAĞ Serpil,BEYTUT ENVER,ÇELEBİ Özgür,KARATAŞ Özhan,NUHOĞLU Hilmi,MENDİL Ali Sefa,AKÇA Doğan,YILDIZ Ayfer Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. (2021): 33 - 39. 10.35864/evmd.748570 |
| MLA | KARAKURT Emin,BÜYÜK Fatih,DAĞ Serpil,BEYTUT ENVER,ÇELEBİ Özgür,KARATAŞ Özhan,NUHOĞLU Hilmi,MENDİL Ali Sefa,AKÇA Doğan,YILDIZ Ayfer Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. , 2021, ss.33 - 39. 10.35864/evmd.748570 |
| AMA | KARAKURT E,BÜYÜK F,DAĞ S,BEYTUT E,ÇELEBİ Ö,KARATAŞ Ö,NUHOĞLU H,MENDİL A,AKÇA D,YILDIZ A Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. . 2021; 33 - 39. 10.35864/evmd.748570 |
| Vancouver | KARAKURT E,BÜYÜK F,DAĞ S,BEYTUT E,ÇELEBİ Ö,KARATAŞ Ö,NUHOĞLU H,MENDİL A,AKÇA D,YILDIZ A Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. . 2021; 33 - 39. 10.35864/evmd.748570 |
| IEEE | KARAKURT E,BÜYÜK F,DAĞ S,BEYTUT E,ÇELEBİ Ö,KARATAŞ Ö,NUHOĞLU H,MENDİL A,AKÇA D,YILDIZ A "Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method." , ss.33 - 39, 2021. 10.35864/evmd.748570 |
| ISNAD | KARAKURT, Emin vd. "Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method". (2021), 33-39. https://doi.org/10.35864/evmd.748570 |
| APA | KARAKURT E, BÜYÜK F, DAĞ S, BEYTUT E, ÇELEBİ Ö, KARATAŞ Ö, NUHOĞLU H, MENDİL A, AKÇA D, YILDIZ A (2021). Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. Etlik Veteriner Mikrobiyoloji Dergisi, 32(1), 33 - 39. 10.35864/evmd.748570 |
| Chicago | KARAKURT Emin,BÜYÜK Fatih,DAĞ Serpil,BEYTUT ENVER,ÇELEBİ Özgür,KARATAŞ Özhan,NUHOĞLU Hilmi,MENDİL Ali Sefa,AKÇA Doğan,YILDIZ Ayfer Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. Etlik Veteriner Mikrobiyoloji Dergisi 32, no.1 (2021): 33 - 39. 10.35864/evmd.748570 |
| MLA | KARAKURT Emin,BÜYÜK Fatih,DAĞ Serpil,BEYTUT ENVER,ÇELEBİ Özgür,KARATAŞ Özhan,NUHOĞLU Hilmi,MENDİL Ali Sefa,AKÇA Doğan,YILDIZ Ayfer Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. Etlik Veteriner Mikrobiyoloji Dergisi, vol.32, no.1, 2021, ss.33 - 39. 10.35864/evmd.748570 |
| AMA | KARAKURT E,BÜYÜK F,DAĞ S,BEYTUT E,ÇELEBİ Ö,KARATAŞ Ö,NUHOĞLU H,MENDİL A,AKÇA D,YILDIZ A Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. Etlik Veteriner Mikrobiyoloji Dergisi. 2021; 32(1): 33 - 39. 10.35864/evmd.748570 |
| Vancouver | KARAKURT E,BÜYÜK F,DAĞ S,BEYTUT E,ÇELEBİ Ö,KARATAŞ Ö,NUHOĞLU H,MENDİL A,AKÇA D,YILDIZ A Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method. Etlik Veteriner Mikrobiyoloji Dergisi. 2021; 32(1): 33 - 39. 10.35864/evmd.748570 |
| IEEE | KARAKURT E,BÜYÜK F,DAĞ S,BEYTUT E,ÇELEBİ Ö,KARATAŞ Ö,NUHOĞLU H,MENDİL A,AKÇA D,YILDIZ A "Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method." Etlik Veteriner Mikrobiyoloji Dergisi, 32, ss.33 - 39, 2021. 10.35864/evmd.748570 |
| ISNAD | KARAKURT, Emin vd. "Investigation of ruminant encephalitic and septicemic listeriosis by the immunofluorescence method". Etlik Veteriner Mikrobiyoloji Dergisi 32/1 (2021), 33-39. https://doi.org/10.35864/evmd.748570 |
