Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR

ERTAŞ, Hasan Basri; KILIÇ, Ayşe; MUZ, Adile; ÖZEY, Gökben

Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR

Hasan Basri ERTAŞ, (Fırat Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ, Türkiye)

Ayşe KILIÇ, (Fırat Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ, Türkiye)

Gökben ÖZEY, (Fırat Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ, Türkiye)

Adile MUZ (Fırat Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Elazığ, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

4 0

Yıl: 2005 Cilt: 29 Sayı: 2 Sayfa Aralığı: 455 - 459 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR

Öz:

Bu çalışmada, deri, eklem ve iç organlarda çeşitli prulent infeksiyonlara sebep olan Arcanobacterium spp.'nin apseli sığır böbreklerindeki varlığı araştırıldı. Toplam 500 sığır postmortem olarak muayene edildi ve apseli olan 100 örnek toplandı. Arcanobacterium pyogenes 40 (% 40) numuneden izole edildi. Izolatlardan elde edilen DNA'lar A. pyogenes'in plo geninden türetilen spesifik primerler kullanılarak Polimeraz Zincir Reaksiyonunda (PZR) çoğaltıldılar ve tüm izolatların PZR'da pozitif olduğu saptandı. Bu sonuç çalışmada kullanılan PZR yönteminin A. pyogenes'in identifikasyonunda uygulanabileceğini göstermiştir.

Anahtar Kelime: sığır DNA çoğaltımı Arcanobacterium pyogenes böbrekler apse moleküler genetik tanı teknikleri izolasyon bakteriyel hastalıklar polimeraz zincir reaksiyonu

Konular: Ziraat Mühendisliği

Apseli sığır böbreklerinden Arcanobacterium (Actinomyces) pyogenes izolasyonu ve PCR ile identifikasyonu

Öz:

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

Anahtar Kelime: abscesses molecular genetics diagnostic techniques isolation bacterial diseases polymerase chain reaction cattle DNA amplification Arcanobacterium pyogenes kidneys

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ERTAŞ H, KILIÇ A, ÖZEY G, MUZ A (2005). Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. , 455 - 459.
Chicago	ERTAŞ Hasan Basri,KILIÇ Ayşe,ÖZEY Gökben,MUZ Adile Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. (2005): 455 - 459.
MLA	ERTAŞ Hasan Basri,KILIÇ Ayşe,ÖZEY Gökben,MUZ Adile Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. , 2005, ss.455 - 459.
AMA	ERTAŞ H,KILIÇ A,ÖZEY G,MUZ A Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. . 2005; 455 - 459.
Vancouver	ERTAŞ H,KILIÇ A,ÖZEY G,MUZ A Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. . 2005; 455 - 459.
IEEE	ERTAŞ H,KILIÇ A,ÖZEY G,MUZ A "Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR." , ss.455 - 459, 2005.
ISNAD	ERTAŞ, Hasan Basri vd. "Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR". (2005), 455-459.

APA	ERTAŞ H, KILIÇ A, ÖZEY G, MUZ A (2005). Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. Turkish Journal of Veterinary and Animal Sciences, 29(2), 455 - 459.
Chicago	ERTAŞ Hasan Basri,KILIÇ Ayşe,ÖZEY Gökben,MUZ Adile Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. Turkish Journal of Veterinary and Animal Sciences 29, no.2 (2005): 455 - 459.
MLA	ERTAŞ Hasan Basri,KILIÇ Ayşe,ÖZEY Gökben,MUZ Adile Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. Turkish Journal of Veterinary and Animal Sciences, vol.29, no.2, 2005, ss.455 - 459.
AMA	ERTAŞ H,KILIÇ A,ÖZEY G,MUZ A Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. Turkish Journal of Veterinary and Animal Sciences. 2005; 29(2): 455 - 459.
Vancouver	ERTAŞ H,KILIÇ A,ÖZEY G,MUZ A Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR. Turkish Journal of Veterinary and Animal Sciences. 2005; 29(2): 455 - 459.
IEEE	ERTAŞ H,KILIÇ A,ÖZEY G,MUZ A "Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR." Turkish Journal of Veterinary and Animal Sciences, 29, ss.455 - 459, 2005.
ISNAD	ERTAŞ, Hasan Basri vd. "Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR". Turkish Journal of Veterinary and Animal Sciences 29/2 (2005), 455-459.