Aims: This study aimed to examine the development of the perceptions of the psychologicalempowerment and patient safety culture among Turkish nurses by implementing a training-basedempowerment program for unit charge nurses.Methods: A pre-experimental, one-group pretest-posttest test design was used. The sample consistedof (n = 38) unit charge nurses and (n = 174) staff nurses; the program included theoretical components,group work, and individual study assignments. Data was collected using the Demographic Data Sheet,Psychological Empowerment Scale and Hospital Survey on Patient Safety Culture before, immediatelyafter, and four months after the program. Data were analysed by percentages, repeated measuresanalysis of variance, and the Friedman analysis was used for the abnormally distributed data. TheCochran's Q test was used to analyze the number of events reported.Results: There was a statistically significant increase found in the results of the PsychologicalEmpowerment Scale's scores on the competence and meaning subscales, the mean positive responseto teamwork within the unit’s composite of Hospital Survey on Patient Safety Culture (p < 0.05), and eventreporting (p < 0.001).Conclusion: The results of this study suggest that creating a positive patient safety culture requires areview of the structures and processes of an organization. Creating, maintaining a desirable patient safetyculture in health institutions is a long and hard process. The duration of future empowerment programsmay be determined to allow for the long-term monitoring of their effects.