DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN

Kalkanci, Ayse; BALTACI, Nurnehir

doi:10.30621/jbachs.920675

DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN

Nurnehir BALTACI, (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Ayşe KALKANCI (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Journal of Basic and Clinical Health Sciences

0 0

Yıl: 2021 Cilt: 5 Sayı: 2 Sayfa Aralığı: 115 - 123 Metin Dili: İngilizce DOI: 10.30621/jbachs.920675 İndeks Tarihi: 29-07-2022

DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN

Öz:

Purpose: Echinocandin resistance is causing problems in the treatment. FKS gene mutations weredetected on the genomes of the resistant Candida strains exposed to echinocandins. The aim of thisstudy was to compare the resistance of reference Candida strains exposed to caspofungin and toinvestigate whether there was a difference in virulence factors between exposed and non-exposed withcaspofungin.Methods: Caspofungin susceptible reference strains, exposed to caspofungin during their incubation incaspofungin containing agar plates at concentrations of 16-0.03 µg/ml. MIC values of caspofungin weredetermined all strains. FKS gene regions of the caspofungin exposed strains were sequenced in order toreveal the difference between the reference strains. Relationship between caspofungin exposure andvirulence properties were investigated in vivo and in vitro methodologies. The in vivo virulence study onthe mini-experimental animal model Galleria mellonella (wax moth) was performed.Results: MIC values of the caspofungin exposed strains were found to be increased as one/two folddilution when compared to the sensitive reference strains. The result of FKS gene sequence no mutationis detected in the reference strains, while a point mutation in caspofungin exposed C. glabrata strainswere detected. No significant difference was found in virulence properties of reference strains andcaspofungin exposed strains.Conclusions: In conclusion, it was understood that the fact that sensitive strains were exposed tocaspofungin even at low concentrations caused an increase in MIC, and that virulence and drugresistance should be evaluated independently.

Anahtar Kelime: Caspofungin Galleria mellonella FKS mutation Virulence Candida

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Touil HF., Boucherit K, Boucherit-Otmani Z et al. Optimum Inhibition of Amphotericin-B-Resistant Candida albicans Strain in Single-and MixedSpecies Biofilms by Candida and Non-Candida Terpenoids. Biomolecules. 2020;10:342.
2. Perlin DS. Mechanisms of echinocandin antifungal drug resistance. Ann New York Acad Sci 2015;1354:1-11.
3. Beyer R, Spettel K, Zeller I et al. Antifungal susceptibility of yeast bloodstream isolates collected during a 10-year period in Austria. Mycoses. 2019;62:357-367.
4. Pfaller MA, Diekema DJ, Turnidge JD et al. Twenty Years of the SENTRY Antifungal Surveillance Program: Results for Candida Species From 1997–2016. Open Forum Infect Dis 2019;6 Suppl 1:79-94.
5. Toutounji M, Tokajian S, Khalaf RA. Genotypic and phenotypic characterization of Candida albicans Lebanese hospital isolates resistant and sensitive to caspofungin. Fungal Genet Biol 2019;127:12-22.
6. Bordallo-Cardona MÁ, Escribano P, de la Pedrosa EGG et al. In vitro exposure to increasing micafungin concentrations easily promotes echinocandin resistance in Candida glabrata isolates. Antimicrob Agents Ch. 2017; 61: e01542-16.
7. Chassot F, Venturini TP, Piasentin FB et al. Exploring the in vitro resistance of Candida parapsilosis to echinocandins. Mycopathologia. 2016;181:663-70.
8. Shields RK, Kline EG, Healey KR, et al. Spontaneous mutational frequency and FKS mutation rates vary by echinocandin agent against Candida glabrata. Antimicrob Agents Ch. 2019;63:e01692-18.
9. Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts- Third Edition: M27–A3. NCCLS, Wayne, PA.2008.
10. Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts, Third Edition informational supplement M27–S3. NCCLS, Wayne, PA. 2008.
11. Lackner M, Tscherner M, Schaller M et al. Positions and numbers of FKS mutations in Candida albicans selectively influence in vitro and in vivo susceptibilities to echinocandin treatment. Antimicrob Agents Ch. 2014;58: 3626- 35.
12. Castanheira M, Woosley LN, Diekema DJ et al. Low prevalence of fks1 hot spot 1 mutations in a worldwide collection of Candida strains. Antimicrob Agents Ch. 2010;54:2655-9.
13. Cheung Y-Y, Hui M. Effects of Echinocandins in combination with Nikkomycin Z against invasive Candida albicans bloodstream isolates and the fks mutants. Antimicrob Agents Ch. 2017;61: e00619-17.
14. Dudiuk C, Gamarra S, Leonardeli F et al. Set of classical PCRs for detection of mutations in Candida glabrata FKS genes linked with echinocandin resistance. J Clin Microbiol. 2014; 52:2609-14.
15. de Melo AV, Zuza-Alves D, da Silva-Rocha W et al. Virulence factors of Candida spp. obtained from blood cultures of patients with candidemia attended at tertiary hospitals in Northeast Brazil. J Mycol Med. 2019;292:132-139.
16. Kalkancı A, Fouad A, Erdoğan M et al. Bazı bakteri ve mantarların virülansının araştırılmasında Galleria mellonella’nın in vivo model olarak kullanılması. Mikrobiyol Bul. 2015; 49:366-76.
17. Pappas PG, Kauffman CA, Andes DR et al. Clinical practice guideline for the management of candidiasis: 2016 update by the Infectious Diseases Society of America. Clin Infect Dis. 2015;62:e1-50.
18. Grosset M, Desnos-Ollivier M, Godet C et al. Recurrent episodes of Candidemia due to Candida glabrata, Candida tropicalis and Candida albicans with acquired echinocandin resistance. Med Mycol Case Rep. 2016;14:20-3.
19. Khan Z, Ahmad S, Mokaddas E et al. Development of echinocandin resistance in Candida tropicalis following short-term exposure to caspofungin for empiric therapy. Antimicrob Agents Ch 2018; 62: AAC. 01926-17.
20. Chassot F, Venturini TP, Piasentin FB et al. Activity of antifungal agents alone and in combination against echinocandin-susceptible and-resistant Candida parapsilosis strains. Rev Iberoam Micol. 2019;36:44-7.
21. Pham CD, Iqbal N, Bolden CB et al. The role of FKS mutations in C. glabrata: MIC values, echinocandin resistance and multidrug resistance. Antimicrob Agents Ch. 2014;58: 4690-6.
22. Ellepola AN, Khajah R, Jayatilake S et al. Impact of brief exposure to antifungal agents on the postantifungal effect and hemolysin activity of oral Candida albicans. J Appl Oral Sci. 2015;23:412- 8.
23. Ripeau J-S, Aumont F, Belhumeur P et al. Effect of the echinocandin caspofungin on expression of Candida albicans secretory aspartyl proteinases and phospholipase in vitro. Antimicrob Agents Ch. 2002;46:3096-100.
24. Ellepola ANB, Chandy R, Khan ZU et al. Caspofungin-induced in-vitro post-antifungal effect and its impact on adhesion related traits of oral Candida dubliniensis and Candida albicans isolates. J Microbiol Immunol. 2016;60:160-7.
25. Papp C, Kocsis K, Tóth R et al. EchinocandinInduced Microevolution of Candida parapsilosis Influences Virulence and Abiotic Stress Tolerance. mSphere. 2018;3:e00547-18.
26. Rodrigues CF, Rodrigues ME, Henriques M. Susceptibility of Candida glabrata biofilms to echinocandins: Alterations in the matrix composition. Biofouling. 2018;34:569-7

APA	BALTACI N, Kalkanci A (2021). DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. , 115 - 123. 10.30621/jbachs.920675
Chicago	BALTACI Nurnehir,Kalkanci Ayse DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. (2021): 115 - 123. 10.30621/jbachs.920675
MLA	BALTACI Nurnehir,Kalkanci Ayse DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. , 2021, ss.115 - 123. 10.30621/jbachs.920675
AMA	BALTACI N,Kalkanci A DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. . 2021; 115 - 123. 10.30621/jbachs.920675
Vancouver	BALTACI N,Kalkanci A DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. . 2021; 115 - 123. 10.30621/jbachs.920675
IEEE	BALTACI N,Kalkanci A "DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN." , ss.115 - 123, 2021. 10.30621/jbachs.920675
ISNAD	BALTACI, Nurnehir - Kalkanci, Ayse. "DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN". (2021), 115-123. https://doi.org/10.30621/jbachs.920675

APA	BALTACI N, Kalkanci A (2021). DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. Journal of Basic and Clinical Health Sciences, 5(2), 115 - 123. 10.30621/jbachs.920675
Chicago	BALTACI Nurnehir,Kalkanci Ayse DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. Journal of Basic and Clinical Health Sciences 5, no.2 (2021): 115 - 123. 10.30621/jbachs.920675
MLA	BALTACI Nurnehir,Kalkanci Ayse DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. Journal of Basic and Clinical Health Sciences, vol.5, no.2, 2021, ss.115 - 123. 10.30621/jbachs.920675
AMA	BALTACI N,Kalkanci A DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. Journal of Basic and Clinical Health Sciences. 2021; 5(2): 115 - 123. 10.30621/jbachs.920675
Vancouver	BALTACI N,Kalkanci A DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN. Journal of Basic and Clinical Health Sciences. 2021; 5(2): 115 - 123. 10.30621/jbachs.920675
IEEE	BALTACI N,Kalkanci A "DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN." Journal of Basic and Clinical Health Sciences, 5, ss.115 - 123, 2021. 10.30621/jbachs.920675
ISNAD	BALTACI, Nurnehir - Kalkanci, Ayse. "DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN". Journal of Basic and Clinical Health Sciences 5/2 (2021), 115-123. https://doi.org/10.30621/jbachs.920675