TY  - JOUR
TI  - DEMONSTRATION OF MUTATION DEVELOPMENT AND VIRULENCE CHANGE IN REFERENCE CANDIDA STRAINS EXPOSED TO CASPOFUNGIN
AB  - Purpose: Echinocandin resistance is causing problems in the treatment. FKS gene mutations weredetected on the genomes of the resistant Candida strains exposed to echinocandins. The aim of thisstudy was to compare the resistance of reference Candida strains exposed to caspofungin and toinvestigate whether there was a difference in virulence factors between exposed and non-exposed withcaspofungin.Methods: Caspofungin susceptible reference strains, exposed to caspofungin during their incubation incaspofungin containing agar plates at concentrations of 16-0.03 µg/ml. MIC values of caspofungin weredetermined all strains. FKS gene regions of the caspofungin exposed strains were sequenced in order toreveal the difference between the reference strains. Relationship between caspofungin exposure andvirulence properties were investigated in vivo and in vitro methodologies. The in vivo virulence study onthe mini-experimental animal model Galleria mellonella (wax moth) was performed.Results: MIC values of the caspofungin exposed strains were found to be increased as one/two folddilution when compared to the sensitive reference strains. The result of FKS gene sequence no mutationis detected in the reference strains, while a point mutation in caspofungin exposed C. glabrata strainswere detected. No significant difference was found in virulence properties of reference strains andcaspofungin exposed strains.Conclusions: In conclusion, it was understood that the fact that sensitive strains were exposed tocaspofungin even at low concentrations caused an increase in MIC, and that virulence and drugresistance should be evaluated independently.
AU  - Kalkanci, Ayse
AU  - BALTACI, Nurnehir
DO  - 10.30621/jbachs.920675
PY  - 2021
JO  - Journal of Basic and Clinical Health Sciences
VL  - 5
IS  - 2
SN  - 2458-8938
SP  - 115
EP  - 123
DB  - TRDizin
UR  - http://search/yayin/detay/437482
ER  -