Boron mine ponds: metagenomic insight to bacterial diversity

NURAL YAMAN, BELMA; Aytar Çelik, Pınar; Korkmaz, Ferhan; Gedikli, Serap; Cabuk, Ahmet; Mutlu, Mehmet Burcin

doi:10.46309/biodicon.2021.902221

Boron mine ponds: metagenomic insight to bacterial diversity

Pınar AYTAR ÇELİK, (Eskişehir Osmangazi Üniversitesi, Çevre Koruma ve Kontrol Programı, Eskişehir, Türkiye)

Mehmet Burçin MUTLU, (Eskişehir Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Ferhan KORKMAZ, (Eskişehir Osmangazi Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Belma NURAL YAMAN, (Eskişehir Osmangazi Üniversitesi, Biyomedikal Mühendisliği Bölümü, Eskişehir, Türkiye)

Serap GEDİKLİ, (Eskişehir Osmangazi Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Ahmet ÇABUK (Eskişehir Osmangazi Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Biological Diversity and Conservation

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Yıl: 2021 Cilt: 14 Sayı: 2 Sayfa Aralığı: 229 - 235 Metin Dili: İngilizce DOI: 10.46309/biodicon.2021.902221 İndeks Tarihi: 29-07-2022

Boron mine ponds: metagenomic insight to bacterial diversity

Öz:

Since it can be a source of new microorganisms with biological potential, it is important to identify the microorganisms found in environments with high boron content in terms of ecological and biotechnological application potentials. In this context, deposit waters in environments where boron mining activities perform are important habitats for boronophilic/boronotolerant microorganisms.In this study, bacterial community in the waste mining ponds of Balıkesir-Bigadiç and Eskişehir-Kırka Boron Mining Operations were investigated by using 16S rDNA gene-targeted Illumina MiSeq sequencing. The greater parts of high-throughput sequences were related to Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia and Actinobacteria phyla. Cyanobacteria and Parcubacteria were other notable groups with low abundance. Genera belonged to Blastopirellula, Luteolibacter, Porhyrobacter and Hydrogenophaga were the most abundant taxa for all the samples. Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter and Pontimonas genera-affiliated reads were also detected at in the October samples. Striking seasonal variations were detected between samples in terms of the type and number of microbial populations.

Anahtar Kelime: high throughput sequencing microbial diversity boron

Bor maden göletleri: bakteriyel çeşitliliğe metagenomik bakış

Öz:

Biyolojik potansiyeli olan yeni mikroorganizmaların kaynağı olabileceği için, bor içeriği yüksek ortamlarda bulunan mikroorganizmaların belirlenmesi ekolojik ve biyoteknolojik uygulama potansiyelleri açısından önemlidir. Bu bağlamda, bor madenciliği faaliyetlerinin gerçekleştirildiği çevrelerdeki birikinti suları boronofilik / boronotolerant mikroorganizmalar için önemli habitatlardır.Bu çalışmada, Balıkesir-Bigadiç ve Eskişehir-Kırka Bor Maden İşletmeleri atık madenciliği havuzlarındaki bakteri topluluğu, 16S rDNA gen hedefli Illumina MiSeq dizilimi kullanılarak incelenmiştir. Yüksek verimli dizilerin büyük kısımları Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia ve Actinobacteria filumları ile ilişkili bulunmuştur. Cyanobacteria ve Parcubacteria, düşük miktara sahip diğer önemli gruplar arasındadır. Blastopirellula, Luteolibacter, Porhyrobacter ve Hydrogenophaga'ya ait cinsler tüm örneklerde en çok bulunan taksonlardır. Ekim örneklerinde Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter ve Pontimonas cinsine bağlı okumalar da tespit edilmiştir. Mikrobiyal popülasyonların türü ve sayısı açısından örnekler arasında çarpıcı mevsimsel farklılıklar tespit edilmiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Aytar Çelik P, Mutlu M, Korkmaz F, NURAL YAMAN B, Gedikli S, Cabuk A (2021). Boron mine ponds: metagenomic insight to bacterial diversity. , 229 - 235. 10.46309/biodicon.2021.902221
Chicago	Aytar Çelik Pınar,Mutlu Mehmet Burcin,Korkmaz Ferhan,NURAL YAMAN BELMA,Gedikli Serap,Cabuk Ahmet Boron mine ponds: metagenomic insight to bacterial diversity. (2021): 229 - 235. 10.46309/biodicon.2021.902221
MLA	Aytar Çelik Pınar,Mutlu Mehmet Burcin,Korkmaz Ferhan,NURAL YAMAN BELMA,Gedikli Serap,Cabuk Ahmet Boron mine ponds: metagenomic insight to bacterial diversity. , 2021, ss.229 - 235. 10.46309/biodicon.2021.902221
AMA	Aytar Çelik P,Mutlu M,Korkmaz F,NURAL YAMAN B,Gedikli S,Cabuk A Boron mine ponds: metagenomic insight to bacterial diversity. . 2021; 229 - 235. 10.46309/biodicon.2021.902221
Vancouver	Aytar Çelik P,Mutlu M,Korkmaz F,NURAL YAMAN B,Gedikli S,Cabuk A Boron mine ponds: metagenomic insight to bacterial diversity. . 2021; 229 - 235. 10.46309/biodicon.2021.902221
IEEE	Aytar Çelik P,Mutlu M,Korkmaz F,NURAL YAMAN B,Gedikli S,Cabuk A "Boron mine ponds: metagenomic insight to bacterial diversity." , ss.229 - 235, 2021. 10.46309/biodicon.2021.902221
ISNAD	Aytar Çelik, Pınar vd. "Boron mine ponds: metagenomic insight to bacterial diversity". (2021), 229-235. https://doi.org/10.46309/biodicon.2021.902221

APA	Aytar Çelik P, Mutlu M, Korkmaz F, NURAL YAMAN B, Gedikli S, Cabuk A (2021). Boron mine ponds: metagenomic insight to bacterial diversity. Biological Diversity and Conservation, 14(2), 229 - 235. 10.46309/biodicon.2021.902221
Chicago	Aytar Çelik Pınar,Mutlu Mehmet Burcin,Korkmaz Ferhan,NURAL YAMAN BELMA,Gedikli Serap,Cabuk Ahmet Boron mine ponds: metagenomic insight to bacterial diversity. Biological Diversity and Conservation 14, no.2 (2021): 229 - 235. 10.46309/biodicon.2021.902221
MLA	Aytar Çelik Pınar,Mutlu Mehmet Burcin,Korkmaz Ferhan,NURAL YAMAN BELMA,Gedikli Serap,Cabuk Ahmet Boron mine ponds: metagenomic insight to bacterial diversity. Biological Diversity and Conservation, vol.14, no.2, 2021, ss.229 - 235. 10.46309/biodicon.2021.902221
AMA	Aytar Çelik P,Mutlu M,Korkmaz F,NURAL YAMAN B,Gedikli S,Cabuk A Boron mine ponds: metagenomic insight to bacterial diversity. Biological Diversity and Conservation. 2021; 14(2): 229 - 235. 10.46309/biodicon.2021.902221
Vancouver	Aytar Çelik P,Mutlu M,Korkmaz F,NURAL YAMAN B,Gedikli S,Cabuk A Boron mine ponds: metagenomic insight to bacterial diversity. Biological Diversity and Conservation. 2021; 14(2): 229 - 235. 10.46309/biodicon.2021.902221
IEEE	Aytar Çelik P,Mutlu M,Korkmaz F,NURAL YAMAN B,Gedikli S,Cabuk A "Boron mine ponds: metagenomic insight to bacterial diversity." Biological Diversity and Conservation, 14, ss.229 - 235, 2021. 10.46309/biodicon.2021.902221
ISNAD	Aytar Çelik, Pınar vd. "Boron mine ponds: metagenomic insight to bacterial diversity". Biological Diversity and Conservation 14/2 (2021), 229-235. https://doi.org/10.46309/biodicon.2021.902221