Regulation of E2F1 activity via PKA-mediated phosphorylations

Mustafa Gökhan ERTOSUN, (Akdeniz Üniversitesi, Tıp Fakültesi, Plastik, Rekonstrüktif ve Estetik Cerrahi Anabilim Dalı, Antalya, Türkiye)
Sayra DİLMAÇ, (Akdeniz Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Antalya, Türkiye)
Fatma Zehra HAPİL, (Akdeniz Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, Antalya, Türkiye)
Gamze TANRIÖVER, (Akdeniz Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Antalya, Türkiye)
Sadi KÖKSOY, (Akdeniz Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Antalya, Türkiye)
Osman Nidai ÖZEŞ (Altay Therapeutics, California, United States of America)
Turkish Journal of Biology
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Yıl: 2020 Cilt: 44 Sayı: 5 Sayfa Aralığı: 215 - 229 Metin Dili: İngilizce DOI: 10.3906/biy-2003-9 İndeks Tarihi: 29-07-2022

Regulation of E2F1 activity via PKA-mediated phosphorylations

Öz:
E2F1 becomes activated during the G1 phase of the cell cycle, and posttranslational modifications modulate its activity.Activation of G-protein coupled receptors (GPCR) by many ligands induces the activation of adenylate cyclases and the production ofcAMP, which activates the PKA enzyme. Activated PKA elicits its biological effect by phosphorylating the target proteins containingserine or threonine amino acids in the RxxS/T motif. Since PKA activation negatively regulates cell proliferation, we thought thatactivated PKA would negatively affect the activity of E2F1. In line with this, when we analyzed the amino acid sequence of E2F1,we found 3 hypothetical consensus PKA phosphorylation sites located at 127-130, 232-235, and 361-364 positions and RYET, RLLS,and RMGS sequences. After showing the binding and phosphorylation of E2F1 by PKA, we converted the codons of Threonine-130,Serine-235, and Serine-364 to Alanine and Glutamic acid codons on the eukaryotic E2F1 expression vector we had previously created.We confirmed the phosphorylation of T130, S235, and S364 by developing monoclonal antibodies against phospho-specific forms ofthese sites and showed that their phosphorylation is cell cycle-dependent. According to our results, PKA-mediated phosphorylation ofE2F1 by PKA inhibits proliferation and glucose uptake and induces caspase-3 activation and senescence.
Anahtar Kelime: forskolin senescence E2F1 proliferation cell cycle PKA

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ERTOSUN M, DILMAÇ S, Hapil F, tanriover g, KOKSOY S, ozes o (2020). Regulation of E2F1 activity via PKA-mediated phosphorylations. , 215 - 229. 10.3906/biy-2003-9
Chicago ERTOSUN Mustafa Gokhan,DILMAÇ SAYRA,Hapil Fatma Zehra,tanriover gamze,KOKSOY SADI,ozes osman nidai Regulation of E2F1 activity via PKA-mediated phosphorylations. (2020): 215 - 229. 10.3906/biy-2003-9
MLA ERTOSUN Mustafa Gokhan,DILMAÇ SAYRA,Hapil Fatma Zehra,tanriover gamze,KOKSOY SADI,ozes osman nidai Regulation of E2F1 activity via PKA-mediated phosphorylations. , 2020, ss.215 - 229. 10.3906/biy-2003-9
AMA ERTOSUN M,DILMAÇ S,Hapil F,tanriover g,KOKSOY S,ozes o Regulation of E2F1 activity via PKA-mediated phosphorylations. . 2020; 215 - 229. 10.3906/biy-2003-9
Vancouver ERTOSUN M,DILMAÇ S,Hapil F,tanriover g,KOKSOY S,ozes o Regulation of E2F1 activity via PKA-mediated phosphorylations. . 2020; 215 - 229. 10.3906/biy-2003-9
IEEE ERTOSUN M,DILMAÇ S,Hapil F,tanriover g,KOKSOY S,ozes o "Regulation of E2F1 activity via PKA-mediated phosphorylations." , ss.215 - 229, 2020. 10.3906/biy-2003-9
ISNAD ERTOSUN, Mustafa Gokhan vd. "Regulation of E2F1 activity via PKA-mediated phosphorylations". (2020), 215-229. https://doi.org/10.3906/biy-2003-9
APA ERTOSUN M, DILMAÇ S, Hapil F, tanriover g, KOKSOY S, ozes o (2020). Regulation of E2F1 activity via PKA-mediated phosphorylations. Turkish Journal of Biology, 44(5), 215 - 229. 10.3906/biy-2003-9
Chicago ERTOSUN Mustafa Gokhan,DILMAÇ SAYRA,Hapil Fatma Zehra,tanriover gamze,KOKSOY SADI,ozes osman nidai Regulation of E2F1 activity via PKA-mediated phosphorylations. Turkish Journal of Biology 44, no.5 (2020): 215 - 229. 10.3906/biy-2003-9
MLA ERTOSUN Mustafa Gokhan,DILMAÇ SAYRA,Hapil Fatma Zehra,tanriover gamze,KOKSOY SADI,ozes osman nidai Regulation of E2F1 activity via PKA-mediated phosphorylations. Turkish Journal of Biology, vol.44, no.5, 2020, ss.215 - 229. 10.3906/biy-2003-9
AMA ERTOSUN M,DILMAÇ S,Hapil F,tanriover g,KOKSOY S,ozes o Regulation of E2F1 activity via PKA-mediated phosphorylations. Turkish Journal of Biology. 2020; 44(5): 215 - 229. 10.3906/biy-2003-9
Vancouver ERTOSUN M,DILMAÇ S,Hapil F,tanriover g,KOKSOY S,ozes o Regulation of E2F1 activity via PKA-mediated phosphorylations. Turkish Journal of Biology. 2020; 44(5): 215 - 229. 10.3906/biy-2003-9
IEEE ERTOSUN M,DILMAÇ S,Hapil F,tanriover g,KOKSOY S,ozes o "Regulation of E2F1 activity via PKA-mediated phosphorylations." Turkish Journal of Biology, 44, ss.215 - 229, 2020. 10.3906/biy-2003-9
ISNAD ERTOSUN, Mustafa Gokhan vd. "Regulation of E2F1 activity via PKA-mediated phosphorylations". Turkish Journal of Biology 44/5 (2020), 215-229. https://doi.org/10.3906/biy-2003-9
Sayfa Oluşturulma Tarihi
23-04-2024 19:9

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