Pathomorphological findings of Mortellaro disease in dairy cattle
Yıl: 2021 Cilt: 68 Sayı: 3 Sayfa Aralığı: 245 - 250 Metin Dili: İngilizce DOI: 10.33988/auvfd.798803 İndeks Tarihi: 29-07-2022
Pathomorphological findings of Mortellaro disease in dairy cattle
Öz: Mortellaro disease is an infection caused by mostly spirochete bacteria in dairy cows, sheep, and goats. The aim ofthe present study was to define to Mortellaro disease pathomorphologically and immunohistochemically and investigate whether cellcell adhesion connections are lost in cell proliferation. Tissue samples were collected from underfoots of Holstein (n: 6) and SwissBrown (n: 15) dairy cattle. The sections were stained with hematoxylin-eosin (H&E) and Warthin-Starry (WS) for isolation ofspirochetes histopathologically and with β-catenin and PCNA primary antibodies immunohistochemically. Histopathologically,number of the cells in the stratum spinosum layer increased excessively, and it gave finger-like extensions to the dermis. Black stainedspirochetes were detected among enlarged keratinocytes and inflammatory cells in WS staining. β-catenin was strongly positive in thecell membranes of epithelial cells in all slides. PCNA also gave positive reaction moderately (n: 18) and strongly (n: 3) in nuclei ofepithelial cells. It was observed that β-catenin cell-cell adhesion connections did not impair in the disease, so this is thought to positivelyaffect the prognosis of the disease. The high expression of PCNA showed that the mitotic activity was high and explained the formationof wart-like. As a result, while the disease does not have a poor prognosis, the disease has a negative economic impact, and theeconomical results of this disease might be much greater than the treatment costs. It is still seen in our country and causes in loss ofyield for the economy of country.
Anahtar Kelime: Süt sığırlarında Mortellaro hastalığının patomorfolojik bulguları
Öz: Mortellaro hastalığı süt sığırlarında, koyunlarda ve keçilerde şekillenen, çoğunlukla spiroket bakterilerinin neden olduğu bir enfeksiyondur. Bu çalışmanın amacı, Mortellaro hastalığını patolojik ve immünohistokimyasal olarak tanımlanması ve hücre proliferasyonunda hücre-hücre adezyon bağlantılarının kaybolup kaybolmadığının araştırılmasıdır. Çalışmada Holştayn (n: 6) ve İsviçre Esmeri (n: 15) ırkı süt sığırlarından ayakların altından doku örnekleri toplandı. Kesitler, histopatolojik olarak hematoksileneozin (H&E) ve spiroketlerin izolasyonu için Warthin-Starry (WS) ile; immünohistokimyasal olarak da β-katenin ve PCNA primer antikorları ile boyandı. Histopatolojik olarak stratum spinosum tabakasındaki hücreler aşırı derecede artmıştı ve dermise parmak benzeri uzantılar vermişti. WS boyamasında genişlemiş keratinositler ve yangı hücreleri arasında siyah renkli spiroketler tespit edildi. β-katenin, tüm olgularda epitel hücrelerinin hücre membranlarında kuvvetli pozitifti. PCNA da epitel hücrelerinin çekirdeklerinde orta derecede (n: 18) ve kuvvetli (n: 3) pozitif reaksiyon verdi. β-katenin hücre-hücre adezyon bağlantılarının hastalıkta kaybolmadığı gözlendi, bu nedenle bu durumun hastalığın prognozunu olumlu etkilediği düşünülmektedir. PCNA'nın yüksek ekspresyonu, mitotik aktivitenin yüksek olduğunu gösterdi ve siğil benzeri oluşumları açıkladı. Sonuç olarak, hastalık kötü bir prognoza sahip değildir ancak ekonomik kayıpları oldukça yüksektir ve bu nedenle bu hastalığın ekonomik sonuçları tedavi maliyetlerinden çok daha fazla olabilir. Ülkemizde halen görülmektedir ve ülke ekonomisinde verim kaybına neden olmaktadır
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | TUNÇ A, ÇAĞATAY S, Saglam M, KUTSAL O (2021). Pathomorphological findings of Mortellaro disease in dairy cattle. , 245 - 250. 10.33988/auvfd.798803 |
| Chicago | TUNÇ ARDA SELIN,ÇAĞATAY Soner,Saglam Mehmet,KUTSAL Osman Pathomorphological findings of Mortellaro disease in dairy cattle. (2021): 245 - 250. 10.33988/auvfd.798803 |
| MLA | TUNÇ ARDA SELIN,ÇAĞATAY Soner,Saglam Mehmet,KUTSAL Osman Pathomorphological findings of Mortellaro disease in dairy cattle. , 2021, ss.245 - 250. 10.33988/auvfd.798803 |
| AMA | TUNÇ A,ÇAĞATAY S,Saglam M,KUTSAL O Pathomorphological findings of Mortellaro disease in dairy cattle. . 2021; 245 - 250. 10.33988/auvfd.798803 |
| Vancouver | TUNÇ A,ÇAĞATAY S,Saglam M,KUTSAL O Pathomorphological findings of Mortellaro disease in dairy cattle. . 2021; 245 - 250. 10.33988/auvfd.798803 |
| IEEE | TUNÇ A,ÇAĞATAY S,Saglam M,KUTSAL O "Pathomorphological findings of Mortellaro disease in dairy cattle." , ss.245 - 250, 2021. 10.33988/auvfd.798803 |
| ISNAD | TUNÇ, ARDA SELIN vd. "Pathomorphological findings of Mortellaro disease in dairy cattle". (2021), 245-250. https://doi.org/10.33988/auvfd.798803 |
| APA | TUNÇ A, ÇAĞATAY S, Saglam M, KUTSAL O (2021). Pathomorphological findings of Mortellaro disease in dairy cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(3), 245 - 250. 10.33988/auvfd.798803 |
| Chicago | TUNÇ ARDA SELIN,ÇAĞATAY Soner,Saglam Mehmet,KUTSAL Osman Pathomorphological findings of Mortellaro disease in dairy cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no.3 (2021): 245 - 250. 10.33988/auvfd.798803 |
| MLA | TUNÇ ARDA SELIN,ÇAĞATAY Soner,Saglam Mehmet,KUTSAL Osman Pathomorphological findings of Mortellaro disease in dairy cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.68, no.3, 2021, ss.245 - 250. 10.33988/auvfd.798803 |
| AMA | TUNÇ A,ÇAĞATAY S,Saglam M,KUTSAL O Pathomorphological findings of Mortellaro disease in dairy cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(3): 245 - 250. 10.33988/auvfd.798803 |
| Vancouver | TUNÇ A,ÇAĞATAY S,Saglam M,KUTSAL O Pathomorphological findings of Mortellaro disease in dairy cattle. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(3): 245 - 250. 10.33988/auvfd.798803 |
| IEEE | TUNÇ A,ÇAĞATAY S,Saglam M,KUTSAL O "Pathomorphological findings of Mortellaro disease in dairy cattle." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, ss.245 - 250, 2021. 10.33988/auvfd.798803 |
| ISNAD | TUNÇ, ARDA SELIN vd. "Pathomorphological findings of Mortellaro disease in dairy cattle". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/3 (2021), 245-250. https://doi.org/10.33988/auvfd.798803 |
