Molecular identification of Paramphistomidae obtained from ruminants in Van province
Yıl: 2021 Cilt: 68 Sayı: 3 Sayfa Aralığı: 223 - 228 Metin Dili: İngilizce DOI: 10.33988/auvfd.727842 İndeks Tarihi: 29-07-2022
Molecular identification of Paramphistomidae obtained from ruminants in Van province
Öz: This study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecularmethods between March 2018 and September 2018 at Van municipality slaughterhouse. In the research, the rDNA ITS-2 gene regionof adult Paramphistomums was amplified by PCR method. Amplicons 399 bp long were viewed in agarose gel. As a result ofbidirectional sequence analysis made from PCR amplicons, Paramphistomum leydeni and Calicophoron daubneyi species wereidentifyed. Amplicon sequences were compared by BLAST with reference sequences from Genbank. Phylogenetic tree was createdwith the Neighbor-Joining method by using the MEGA 7 program. Genotypes obtained from isolates were compared for exact orclosest similarities. In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, itwas identified for the first time by using molecular methods. Also in this study, P. leydeni was reported for the first time in Turkey.
Anahtar Kelime: Van ilindeki ruminatlardan elde edilen Paramphistomidae’lerin moleküler olarak belirlenmesi
Öz: Bu çalışmada, Mart 2018-Eylül 2018 tarihleri arasında Van belediyesi mezbahasında enfekte ruminantlardan (koyun ve sığır) elde edilen Paramphistomum türlerinin moleküler yöntem kullanılarak belirlenmesi amaçlanmıştır. Araştırmada, erişkin Paramphistomum’ların rDNA ITS-2 gen bölgesi PCR yöntemi ile çoğaltıldı. Agaroz jelde, 399 bp uzunluğunda amplikonlar görüntülendi. PCR amplikonlarından yapılan çift yönlü sekans analizi sonucunda Paramphistomum leydeni ve Calicophoron daubneyi türleri belirlendi. Amplicon dizileri, BLAST ile Genbank'taki referans dizilerle karşılaştırıldı. MEGA 7 programı kullanılarak Neighbor-Joining yöntemi ile filogenetik ağaç oluşturuldu. İzolatlardan elde edilen genotipler tam yada en yakın benzerlikleri karşılaştırıldı. Sonuç olarak, Türkiye'de daha önce morfolojik yöntemlerle tespit edilen C. daubneyi, bu çalışmada moleküler yöntemler kullanılarak ilk kez tespit edildi. Ayrıca bu çalışmada, Türkiye'de ilk kez P. leydeni bildirildi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Anderson GR, Barker SC (1998): Inference of phylogeny and taxonomy within the didymozoidae (Digenea) from the second internal transcribed spacer ITS2 of ribosomal DNA. Syst Parasitol, 41, 87-94.
- 2. Celep A, Açıcı M, Çetindağ M, et al (1990): Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Vet Mikrobiyol Derg, 6, 117-30.
- 3. Coşkun ŞZ (1988): The discovery and spread of Paramphistomum species in ruminants.Turk J Vet Anim Sci, 12, 168-179.
- 4. Goswami LM, Prasad PK, Tandon V, et al (2009): Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis. Parasitol Res, 104, 1485-1490.
- 5. Güralp N (1981): The Helminthology. Ankara University Faculty of Veterinary Medicine. Ankara University Press, Ankara, Turkey.
- 6. Horak IG (1971): Paramphistomiasis of domestic ruminants. Adv Parasitol, 9, 33-72.
- 7. Ichikawa M, Kondoh D, Bawn S, et al (2013): Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar. JVMS, 75, 309-314.
- 8. Itagaki T, Tsumagari N, Tsutsumi K, et al (2003): Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers. J Vet Med Sci, 65, 931-933.
- 9. Jones A (1990): Techniques for hand-sectioning thickbodied Platyhelminths. Syst Parasitol, 15, 211-218.
- 10. Khan UJ (2008): Epidemiology, Economic Importance and Therapy of Paramphistomosis in Cattle and Buffaloes. PhD Thesis Department of zoology, University of Punjap, Lahor, Pakistan.
- 11. Lotfy WM, Brant SV, Ashmawy KI, et al (2010): A molecular approach for identification of paramphistomes from Africa and Asia. Vet Parasitol, 174, 234-240.
- 12. Lotfy WM, Brant SV, DeJong RJ, et al (2008): Evolutionary origins, diversification, and biogeography of liver flukes (Digenea, Fasciolidae). Am J Trop Med Hyg, 79, 248-255.
- 13. Luton K, Walker D, Blair D (1992): Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Piatyhelminthes: Trematoda: Digenea). Mol Biochem Parasit, 56, 323-328.
- 14. Martínez-Ibeas AM, González-Warleta M, MartínezValladares M, et al (2013): Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species. BMC Vet Res, 12, 143.
- 15. Özdal N, Gül A, İlhan F, et al (2010): Prevalence of Paramphistomum infection in cattle and sheep in Van Province, Turkey. Helminthologia, 47, 20-24.
- 16. Reinitz DM, Yoshino TP, Cole RA (2007): A Ribeiroia spp. (Class: Trematoda)-specific PCR-based diagnostic. J. Parasitol, 93, 1234-1238.
- 17. Rinaldi L, Perugini AG, Capuano F, et al (2005): Characterization of the second internal transcribed spacer of ribosomal DNA of Calicophoron daubneyi from various hosts and locations in southern Italy. Vet Parasitol, 131, 247-253.
- 18. Roy B, Tandon V (1993): Morphological and microtopographical train variations among Fasciolopsis buski originating from different geographical areas. Acta Parasitol, 38, 72-77.
- 19. Sanabria R, More G, Romero J (2011): Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae). Vet Parasitol, 177, 182-185.
- 20. Şenlik B (2013): Helminth Diseases Seen in Digestive System in Cattle. In: Ozcel MA (Ed). Parasitic Diseases in Veterinary Medicine. Meta Printing Printing Services. İzmir.
- 21. Sharma S, Lyngdoh D, Roy B, et al (2016): Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Parasitol Res, 5, 4293-4298.
- 22. Sharma S, Lyngdoh D, Roy B, et al (2016): Molecular phylogeny of Cyclophyllidea (Cestoda:Eucestoda): An insilico analysis based on mtCOI gene. Parasitol Res, 115, 3329-3335.
- 23. Sindičić M, Martinkovic F, Striskovic T, et al (2017): Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus. J Helminthol, 91, 637-641.
- 24. Tandon V, Roy B (2002): SEM Pictorial Guide to Trematodes of Live Stock In India. Regency Publication. New Delhi, India.
- 25. Thompson RCA, Zarlenga DS, La Rosa G (2004): Molecular systematics and diagnosis. Vet Parasitol, 125, 69-92.
- 26. Tınar R, Umur Ş, Köroğlu E, et al (2006): The Helmintology. In: Tınar R (Ed). Nobel, Ankara, Turkey.
- 27. Titi A (2013): La paramphistomose gastro-duodenales des ruminants dans le nord-est algerien: investigations sur les bovins et le mollusque hote PhD thesis, Constantine University, Algeria.
- 28. Van Van K, Dalsgaard A, Blair D, et al (2009): Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adults and larvae. Exp Parasitol, 123, 146-151.
| APA | padak y, karakuş a (2021). Molecular identification of Paramphistomidae obtained from ruminants in Van province. , 223 - 228. 10.33988/auvfd.727842 |
| Chicago | padak yusuf,karakuş ayşe Molecular identification of Paramphistomidae obtained from ruminants in Van province. (2021): 223 - 228. 10.33988/auvfd.727842 |
| MLA | padak yusuf,karakuş ayşe Molecular identification of Paramphistomidae obtained from ruminants in Van province. , 2021, ss.223 - 228. 10.33988/auvfd.727842 |
| AMA | padak y,karakuş a Molecular identification of Paramphistomidae obtained from ruminants in Van province. . 2021; 223 - 228. 10.33988/auvfd.727842 |
| Vancouver | padak y,karakuş a Molecular identification of Paramphistomidae obtained from ruminants in Van province. . 2021; 223 - 228. 10.33988/auvfd.727842 |
| IEEE | padak y,karakuş a "Molecular identification of Paramphistomidae obtained from ruminants in Van province." , ss.223 - 228, 2021. 10.33988/auvfd.727842 |
| ISNAD | padak, yusuf - karakuş, ayşe. "Molecular identification of Paramphistomidae obtained from ruminants in Van province". (2021), 223-228. https://doi.org/10.33988/auvfd.727842 |
| APA | padak y, karakuş a (2021). Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(3), 223 - 228. 10.33988/auvfd.727842 |
| Chicago | padak yusuf,karakuş ayşe Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no.3 (2021): 223 - 228. 10.33988/auvfd.727842 |
| MLA | padak yusuf,karakuş ayşe Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.68, no.3, 2021, ss.223 - 228. 10.33988/auvfd.727842 |
| AMA | padak y,karakuş a Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(3): 223 - 228. 10.33988/auvfd.727842 |
| Vancouver | padak y,karakuş a Molecular identification of Paramphistomidae obtained from ruminants in Van province. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(3): 223 - 228. 10.33988/auvfd.727842 |
| IEEE | padak y,karakuş a "Molecular identification of Paramphistomidae obtained from ruminants in Van province." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68, ss.223 - 228, 2021. 10.33988/auvfd.727842 |
| ISNAD | padak, yusuf - karakuş, ayşe. "Molecular identification of Paramphistomidae obtained from ruminants in Van province". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/3 (2021), 223-228. https://doi.org/10.33988/auvfd.727842 |
