TY  - JOUR
TI  - Influences of uterine tubal factors on oocyte maturation and embryo segmentation
AB  - OBJECTIVE: The aim of this study was to investigate the effects of uterine tubal epithelium or its fluid on in vitro oocyte maturation and embryo development of rats. STUDY DESIGN: For this purpose, mature, immature cumulus oocyte complexes (COC) and embryos from wister albino rats were used. Mature and immature COC of control groups were incubated in 10% EBSS (Earle's Balanced Salt Solution) and control embryos were cultured in 15% EBSS. Test groups were cultured with same culture mediums in which uterine tubal fluid or uterine tubal epithelium was added. Survivals of oocytes and embryos were evaluated with a phase-contrast inverted microscope. In vitro maturation of oocyte and embryo segmentation were observed with light, fluorescence and transmission electron microscopes. RESULTS: When uterine tubal epithelium or its fluid were added to the conventional culture medium, the maturation of immature oocytes was increased and the segmentation ratios of embryos was accelerated. CONCLUSIONS: Addition of uterine tubal epithelium or its fluid to the conventional medium as a source of various endogenous mediators bringing out more alikeness in vivo conditions may play an important role in paracrine communications for better maturation of oocytes and viabilities of pre-implantation embryos.
AU  - AÇIKALIN, A. Ergin
AU  - ŞAHİNTÜRK, Varol
AU  - GÜRER, Firdevs
AU  - BAYÇU, Cengiz
PY  - 2004
JO  - GORM:Gynecology Obstetrics & Reproductive Medicine
VL  - 10
IS  - 1
SN  - 1300-4751
SP  - 39
EP  - 43
DB  - TRDizin
UR  - http://search/yayin/detay/44679
ER  -